Tissue culture and toxicology

Rofe, Philip

doi:10.1016/0015-6264(71)90159-3

Cited by 26 publications

(1 citation statement)

References 36 publications

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“…The metals were subsequently rinsed for 1 min in freon stray (Freon TF).t For purposes of economy, metals [1][2][3][4][5][6] (Table I) were reused and therefore washed, dried, and freon-rinsed between assays. To prevent oxidation, silver (material 2, Table I) was stored in an evacuated dessictator after washing and before testing.…”

Section: Test Materials Preparationmentioning

confidence: 99%

Biocompatibility test procedures for materials evaluation in vitro. I. Comparative test system sensitivity

Johnson¹,

Northup²,

Seagraves³

et al. 1983

J. Biomed. Mater. Res.

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Relative sensitivity of in vitro biocompatibility test systems was explored. Cellular responses of 12 standardized cell lines to 20 materials representing a range of toxicity were measured. Each cell line and material combination was tested in duplicate using four different culture methods, and each culture plate was examined by two graders. Results of the tissue culture assays were compared to those obtained for the same materials in vivo using a 5-day rabbit intramuscular implant assay. Methods involving measurement of cellular growth (colony counts or percent of confluence) in serum-fortified media extracts of test samples were generally more sensitive and discriminating than those in which test materials were placed directly in cell cultures (measurement of zone of growth inhibition). There was good agreement between graders for all test methods. Antibiotics were not used in the test program. Based upon sensitivity, reproducibility, ability to discriminate materials, and grader agreement, 4 of the 12 cell lines and 2 of the 4 test methods appeared most suitable for screening and evaluation of materials. Agreement of results using these four cell lines with intramuscular implantation tests for the 30 materials ranged from 60 to 90%.

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Section: Test Materials Preparationmentioning

confidence: 99%

Biocompatibility test procedures for materials evaluation in vitro. I. Comparative test system sensitivity

Johnson¹,

Northup²,

Seagraves³

et al. 1983

J. Biomed. Mater. Res.

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Application of In Vitro Techniques in Drug Safety Assessment

2010

Drug Safety Evaluation

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Application of In Vitro Techniques in Drug Safety Assessment

2010

Pharmaceutical Sciences Encyclopedia

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There has been progress in the development of in vitro test systems for toxicity evaluations of drugs. The use of in vitro test systems demonstrates the pharmaceutical industry's commitment to the reduction, refinement, and replacement of whole‐animal in vivo tests whenever possible, while balancing this with the need to accurately assess the safety of new products and to protect the public from harm. This article focuses on testing objectives, design, and industrial applications of in vitro test systems in drug safety assessment.

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Tissue culture and toxicology

Cited by 26 publications

References 36 publications

Biocompatibility test procedures for materials evaluation in vitro. I. Comparative test system sensitivity

Biocompatibility test procedures for materials evaluation in vitro. I. Comparative test system sensitivity

Application of In Vitro Techniques in Drug Safety Assessment

Application of In Vitro Techniques in Drug Safety Assessment

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