Tissue Culture and Plant Regeneration in Diploid Perennial Teosinte

Prioli, L. M.; Silva, W.J.; Söndahl, M. R.

doi:10.1016/s0176-1617(84)80033-4

Cited by 5 publications

(8 citation statements)

References 11 publications

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“…In some calli nodular white tissue was present in the callus mass, similar to that previously reported for Zea diploperennis L . (Prioli et al ., 1984) and maize (Green & Phillips, 1975) .…”

Section: Resultsmentioning

confidence: 99%

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Somatic embryogenesis and plant regeneration of Tripsacum dactyloides L.

Furini

Jewell

1991

Euphytica

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This article reports the culture and plant regeneration of Tripsacum dactyloides . Mature embryos of Tripsacum dactyloides dactyloides were used to obtain embryogenic callus cultures . Currently, 180 normal plants have been regenerated from these cultures . Callus was initiated on MS medium supplemented with dicamba (10 µmol or 20 µmol) and sucrose (3% or 6%), and plants were regenerated on hormone free MS medium containing 2% sucrose . No significant differences were found in callus initiation frequency or in embryogenic response of cultures on the four combinations of sucrose and dicamba tested . The embryogenic cultures have been maintained for 9 months (12 subcultures) and have retained regeneration capacity . Plants regenerated from tissue culture of maize-by-Tripsacum hybrids could be useful in maize improvement .

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Section: Resultsmentioning

confidence: 99%

“…In vitro techniques have been successfully applied to Zea diploperennis, the closest wild relative of maize (Prioli et al ., 1984 ;Swedlund & Locy, 1988), but tissue culture response of the distantly related genus Tripsacum has not been reported .…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration of Tripsacum dactyloides L.

Furini

Jewell

1991

Euphytica

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“…They were unable to get regenerated shoots on hormone-free media ( Cure and Mott, 1978 ). One group published two manuscripts in 1984, reporting the production of embryogenic callus from field grown seedling leaf pieces ( Prioli et al, 1984 ; Sondahl et al, 1984 ) or immature embryos ( Sondahl et al, 1984 ) using MS salts containing 2,4-D in Zea mays ssp. diploperennis (hereafter Zea diploperennis ).…”

Section: Introductionmentioning

confidence: 99%

“…diploperennis (hereafter Zea diploperennis ). This group was successful in regenerating many fertile plants from these callus cultures by removal of the 2,4-D from the growth media ( Prioli et al, 1984 ; Sondahl et al, 1984 ). Swedlund and Locy (1988) also reported the establishment of embryogenic callus using both immature embryos and immature leaf tissue of Zea diploperennis on MS/sucrose-based growth media containing 2,4-D.…”

Section: Introductionmentioning

confidence: 99%

Transformation of Teosinte (Zea mays ssp. parviglumis) via Biolistic Bombardment of Seedling-Derived Callus Tissues

et al. 2021

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Modern maize exhibits a significantly different phenotype than its wild progenitor teosinte despite many genetic similarities. Of the many subspecies of Zea mays identified as teosinte, Zea mays ssp. parviglumis is the most closely related to domesticated maize. Understanding teosinte genes and their regulations can provide great insights into the maize domestication process and facilitate breeding for future crop improvement. However, a protocol of genetic transformation, which is essential for gene functional analyses, is not available in teosinte. In this study, we report the establishment of a robust callus induction and regeneration protocol using whorl segments of seedlings germinated from mature seeds of Zea parviglumis. We also report, for the first time, the production of fertile, transgenic teosinte plants using the particle bombardment. Using herbicide resistance genes such as mutant acetolactate synthase (Als) or bialaphos resistance (bar) as selectable markers, we achieved an average transformation frequency of 4.17% (percentage of independent transgenic events in total bombarded explants that produced callus). Expression of visual marker genes of red fluorescent protein tdTomato and β-glucuronidase (gus) could be detected in bombarded callus culture and in T1 and T2 progeny plants. The protocol established in this work provides a major enabling technology for research toward the understanding of this important plant in crop domestication.

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“…Previous studies have examined the production of diploid teosinte via a callus phase. Prioli et al (1984) excised explants from subapical shoot meristems of Z. diploperennis , plated them on Murashige and Skoog (MS) medium with 2,4‐dichlorophenoxyacetic acid (2,4‐D) to induce callus formation, and generated plants from calli. Later, this same group reported high frequencies of plant regeneration with this same system (Sondahl et al, 1984).…”

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confidence: 99%

Micropropagation of Field‐Grown Perennial Teosinte from Node Culture

et al. 2008

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Propagation of perennial diploid teosinte, Zea diploperennis Iltis, Doebley and Guzmán (2n = 2x = 20), and tetraploid teosinte, Z. perennis (Hitchc.) Reeves and Magelsdorf (2n = 4x = 40), is limited by seed availability. For plants grown in the field, a photoperiod response delays flowering until fall, and seeds may not reach maturity. The objective of this study was to develop an in vitro micropropagation protocol for field grown teosinte. Shoot proliferation was induced from nodes split longitudinally and plated on Murashige and Skoog's (MS) medium supplemented with 5 μM 6‐benzyl amino purine and 3% (w/v) sucrose. Both species possess a single axillary bud that generated multiple plants on division. Rooting of shoots was achieved in half‐strength MS medium with and without the addition of 0.4 μM indole‐3‐butyric acid and 2% (w/v) sucrose. Nodes and younger branches of the tetraploid generated significantly more plants than those of the diploid.

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Tissue Culture and Plant Regeneration in Diploid Perennial Teosinte

Cited by 5 publications

References 11 publications

Somatic embryogenesis and plant regeneration of Tripsacum dactyloides L.

Somatic embryogenesis and plant regeneration of Tripsacum dactyloides L.

Transformation of Teosinte (Zea mays ssp. parviglumis) via Biolistic Bombardment of Seedling-Derived Callus Tissues

Micropropagation of Field‐Grown Perennial Teosinte from Node Culture

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