Tissue culture and aromatic profile in Salvia dolomitica Codd

Abstract

In this study, we developed tissue culture protocol of Salvia dolomitica, a particularly fragrant sage and we evaluated the yield and the profile of essential oil (EO) produced from plants grown in vivo compared to in vitro plant material cultured in two light intensity growth conditions. Plant extracts secreted from glandular trichomes are, in Lamiacea, a valuable source of biologically active volatile compounds. Microscopical observations permitted to\ud describe, for the first time, secretory structures from S. dolomitica leaves developed in vitro showing morphological identity to in vivo glands. The yield of EO obtained from light-stressed in vitro plants was higher than which obtained from the non-stressed in vitro plants and threefold higher that\ud in vivo plants. Phytochemical analysis of EOs and volatile compounds showed quali-quantitative differences between\ud S. dolomitica in vitro grown plantlets and those from open field. a-Pinene, b-phellandrene and borneol accumulated\ud predominantly in micropropagated plants while b-caryophyllene, a-guaiene, d-cadinene and valencene in in vivo\ud material. Both EO and SPME analysis had the same trend: the percentage of total sesquiterpenes decreased strongly\ud in vitro, on the contrary the total monoterpenes are abundant. The effect of the high light treatment on aromatic profile\ud demonstrating that this stress can affect the EO composition and this can be exploited by researchers to address the\ud accumulation of valuable amounts of EO

Salvia Spp. Essential Oils against the Arboviruses Vector Aedes albopictus (Diptera: Culicidae): Bioactivity, Composition, and Sensorial Profile—Stage 1

“…The S. sclarea EO evidenced results in partial agreement with those observed by Aćimović and co-workers [34] on clary sage EO from Tajikistan. The S. dolomitica EO was a subject of discord, in fact, Kamatou et al [35] and Bassolino et al [36] found a composition similar to what we found in the current study, while in a more recent study by Ebani [33], eucalyptol was the main compound. Caser underlined a completely different profile where limonene (19.8%), δ-3-Carene (9.1%), and germacrene D (8.6%) dominated together with (E)-β-ocimene (7.39%) and β-caryophyllene (7.9%) [37].…”

Activity of Salvia dolomitica and Salvia somalensis Essential Oils against Bacteria, Molds and Yeasts

“…Taxa of Salvia spp. (sage) are mainly aromatic plants, rich in essential oils (EOs) and widely employed in foods, perfumery and cosmetics [ 1 ]. EOs from Salvia spp.…”

A laboratory high‐throughput glass chamber using dynamic headspace TD‐GC/MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium‐related abiotic stress

“…Finally, other substrates such as soil (inside the bottles) could be used as an alternative model of plant growth, leading to new possibilities for investigation. The most recent literature describes in vitro studies of VOCs using SPME techniques leading to difficult quantitative interpretations or using destructive methods such as incubation of the harvested tissue at a non‐biological temperature followed by headspace analysis . The glass chamber set‐up was based on preliminary tests focusing on the size of the cuvette system, the development‐age pattern of the plantlets (i.e.…”

“…Conversely, in vitro headspace analysis in connection with abiotic stress studies is usually performed under laboratory conditions. A number of studies described in recent literature have used methods involving plant biological sample destruction such as volatile extraction under air‐dried conditions at 40°C for 72 h, or hydrodistillation performed from cut plant materials . We therefore decided to develop a glass collection chamber that would not harm the plant during its in vitro physiological development or during the VOC sampling procedure.…”

“…A number of studies described in recent literature have used methods involving plant biological sample destruction such as volatile extraction under air-dried conditions at 40°C for 72 h, or hydrodistillation performed from cut plant materials. 5,6 We therefore decided to develop a glass collection chamber that would not harm the plant during its in vitro physiological development or during the VOC sampling procedure. Another goal for this innovative in vitro system was to use the plant's clonal regeneration ability, leading to genetically stable propagation.…”