Tissue CA125 and HE4 Gene Expression Levels Offer Superior Accuracy in Discriminating Benign from Malignant Pelvic Masses

Fawzy, Amal; Mohamed, Mohamed R.; Ali, Mohamed A.; El-Magied, Mohamed H Abd; Helal, Amany

doi:10.7314/apjcp.2016.17.1.323

Cited by 12 publications

(7 citation statements)

References 55 publications

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“…In line with the present data, preoperative serum TIMP-1 concentration showed insufficient diagnostic power (AUC = 0.730) in differentiating between low malignant potential and malignant ovarian tumors [36]. In a few previous publications the AUC values for differentiating ovarian cancer were significantly higher for the combination of various biomarkers [36, 49], which is in line with our findings [13, 28, 43]. Differences in study results might be due to differences in the histological types or disease stages of ovarian cancer and in the number of patients enrolled in each study.…”

Section: Discussionsupporting

confidence: 73%

Plasma levels of MMP-7 and TIMP-1 in laboratory diagnostics and differentiation of selected histological types of epithelial ovarian cancers

et al. 2017

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BackgroundMMP-7 and TIMP-1 may play a role in the pathogenesis of cancer disease. In this study we investigated plasma levels of selected metalloproteinase and its tissue inhibitor in comparison to plasma levels of the commonly accepted tumor markers (CA 125 and HE4) in selected histological types of epithelial ovarian cancer patients as compared to control groups: patients with a benign ovarian tumor and healthy subjects. Plasma levels of MMP-7 and TIMP-1 were determined using ELISA, CA 125 and HE4 – by CMIA methods.ResultsPlasma levels of all biomarkers studied were significantly higher in ovarian cancer patients as compared to both control groups. MMP-7 demonstrated comparable to HE4 or CA125 values of diagnostic sensitivity (SE: 61%; 68%; 58%, respectively), specificity (SP: 95%; 95%; 98%, respectively), positive (PPV: 93%; 96%; 98%, respectively) and negative predictive values (NPV: 61%; 66%; 60%, respectively) in the groups tested. The combined use of the aforementioned biomarkers resulted in a further increase in diagnostic criteria and AUC, especially in the early stages of the disease.ConclusionsThese findings suggest the usefulness of combining MMP-7 with CA 125 and HE4 in the diagnosis of epithelial ovarian cancer as a new tumor marker panel.Electronic supplementary materialThe online version of this article (doi:10.1186/s13048-017-0338-z) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 73%

Plasma levels of MMP-7 and TIMP-1 in laboratory diagnostics and differentiation of selected histological types of epithelial ovarian cancers

et al. 2017

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“…The study also found that the plasma exosomal CA125 and HE4 mRNA levels in the OC group were higher than those in the benign group, but there was no statistical difference from the control group. It was inconsistent with the findings by Fawzy et al that the expression levels of CA125 and HE4 in OC tissues were higher than those of benign tumor tissues and normal ovarian tissues [ 29 ]. In addition, the expression level of plasma exosomal TCF21 in the OC and benign groups was lower than the control group, but there was no difference between the OC and benign groups.…”

Section: Discussioncontrasting

confidence: 74%

Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging

et al. 2022

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Background Ovarian cancer (OC) is one of the serious threats to the health of women worldwide, and accurate biomarkers are urgently demanded for early diagnosis of OC. We have previously confirmed that miR-205 promotes the invasion and metastasis of OC cells by inhibiting the expression of the tumor suppressor gene TCF21. In this study, we used liquid biopsy technology to detect the expression levels of the four genes, miR-205, CA125, HE4 and TCF21, in the exosomes of plasma of OC patients. Combined with analysis of clinicopathological parameters of OC patients, we aimed to provide efficient and non-invasive laboratory biomarkers for early diagnosis of OC. Methods 36 OC patients who were diagnosed in local hospitals from September 2020 to July 2021 were selected as OC group, 31 cases of surgically diagnosed with ovarian benign lesions were selected as benign group, and 32 healthy people who underwent physical examination during the same period were selected as a control group. We employed transmission electron microscope (TEM), Western blotting (WB), and nanoparticle tracking analysis (NTA) to identify biomarkers in the exosomes extracted from plasma of the three groups. The RNA levels of miR-205, CA125, HE4 and TCF21 genes in plasma exosomes were detected by real-time quantitative PCR (qRT-PCR) method. We used clinical pathological parameters and the Receiver Operating Characteristic (ROC) curves to evaluate the diagnostic efficacy for the genes detected in plasma exosomes. Results We found that the expression level of miR-205 in plasma exosomes of the OC group was significantly higher than that of the benign and control groups (P < 0.05), and the level of miR-205 was elevated during the III-IV periods of OC and lymph node metastasis. Conclusion The level of miR-205 in plasma exosomes is a valuable tumor biomarker to improve OC diagnosis.

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“…5 Human epididymis protein 4 (HE4) is a whey acidic four-disulfide core protein formed by 1 single peptide containing 2 whey acidic four-disulfide core domains. Discovered in 1991 by Kirchhoff et al,6 HE4 has been extensively studied for the diagnosis of ovarian cancer 7,8 and just in the latest years has been considered as a promising biomarker for EC. In 2008, Moore et al 9 demonstrated that preoperative HE4 serum levels discriminate EC patients from healthy postmenopausal women, suggesting its promising value as diagnostic serological marker, to be used alone or in combination with cancer antigen 125 (CA125).…”

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confidence: 99%

Preoperative Serum Human Epididymis Protein 4 Levels in Early Stage Endometrial Cancer: A Prospective Study

Fanfani

Restaino

Cicogna

et al. 2017

Int J Gynecol Cancer

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Tissue CA125 and HE4 Gene Expression Levels Offer Superior Accuracy in Discriminating Benign from Malignant Pelvic Masses

Cited by 12 publications

References 55 publications

Plasma levels of MMP-7 and TIMP-1 in laboratory diagnostics and differentiation of selected histological types of epithelial ovarian cancers

Plasma levels of MMP-7 and TIMP-1 in laboratory diagnostics and differentiation of selected histological types of epithelial ovarian cancers

Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging

Preoperative Serum Human Epididymis Protein 4 Levels in Early Stage Endometrial Cancer: A Prospective Study

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