TIRF Microscopy‐Based Monitoring of Drug Permeation Across a Lipid Membrane Supported on Mesoporous Silica

Joyce, Paul; Jõemetsa, Silver; Isaksson, Simon; Hossain, Shakhawath; Larsson, Per; Bergström, Christel A S; Höök, Fredrik

doi:10.1002/ange.202011931

Cited by 7 publications

(3 citation statements)

References 29 publications

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“…Table illustrates that researchers employ FRAP to varying degrees along with other tools to better understand their gel suspension system. In Table , we have also included studies concerning the combination of total internal reflection fluorescence (TIRF) and FRAP. , In this method (TIRF), close to a solid surface (within 100 nm), in water or cell medium that may be modified to prevent stimulating fluorophore away from this margin. Images acquired in this manner will have no background noise and almost no out-of-focus fluorescence.…”

Section: Frap As a Verifying Toolmentioning

confidence: 99%

Fluorescence Recovery after Photobleaching in Colloidal Science: Introduction and Application

Moud

2022

ACS Biomater. Sci. Eng.

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FRAP (fluorescence recovery after photo bleaching) is a method for determining diffusion in material science. In industrial applications such as medications, foods, Medtech, hygiene, and textiles, the diffusion process has a substantial influence on the overall qualities of goods. All these complex and heterogeneous systems have diffusion-based processes at the local level. FRAP is a fluorescence-based approach for detecting diffusion; in this method, a high-intensity laser is made for a brief period and then applied to the samples, bleaching the fluorescent chemical inside the region, which is subsequently filled up by natural diffusion. This brief Review will focus on the existing research on employing FRAP to measure colloidal system heterogeneity and explore diffusion into complicated structures. This description of FRAP will be followed by a discussion of how FRAP is intended to be used in colloidal science. When constructing the current Review, the most recent publications were reviewed for this assessment. Because of the large number of FRAP articles in colloidal research, there is currently a dearth of knowledge regarding the growth of FRAP’s significance to colloidal science. Colloids make up only 2% of FRAP papers, according to ISI Web of Knowledge.

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Section: Frap As a Verifying Toolmentioning

confidence: 99%

Fluorescence Recovery after Photobleaching in Colloidal Science: Introduction and Application

Moud

2022

ACS Biomater. Sci. Eng.

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“…Other applications of TIRF in drug delivery research include the study of the interaction of nanocarriers/nanomaterials designed for nanomedicine applications with synthetic biomimetic membranes. For instance, Conn et al investigated the fusion kinetics of lipid nanocarriers with model membranes and cells through TIRF, thus extending the knowledge of the interaction between drug vectors and cellular barriers [60], while Hook et al recently studied the kinetics of drug permeation through a model membrane by combining TIRF and fluorescence recovery after photobleaching (FRAP) [61].…”

Section: Imaging Thin Layers and Thick Samples: Tirf Lsfmmentioning

confidence: 99%

Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems

et al. 2021

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In the past decade(s), fluorescence microscopy and laser scanning confocal microscopy (LSCM) have been widely employed to investigate biological and biomimetic systems for pharmaceutical applications, to determine the localization of drugs in tissues or entire organisms or the extent of their cellular uptake (in vitro). However, the diffraction limit of light, which limits the resolution to hundreds of nanometers, has for long time restricted the extent and quality of information and insight achievable through these techniques. The advent of super-resolution microscopic techniques, recognized with the 2014 Nobel prize in Chemistry, revolutionized the field thanks to the possibility to achieve nanometric resolution, i.e., the typical scale length of chemical and biological phenomena. Since then, fluorescence microscopy-related techniques have acquired renewed interest for the scientific community, both from the perspective of instrument/techniques development and from the perspective of the advanced scientific applications. In this contribution we will review the application of these techniques to the field of drug delivery, discussing how the latest advancements of static and dynamic methodologies have tremendously expanded the experimental opportunities for the characterization of drug delivery systems and for the understanding of their behaviour in biologically relevant environments.

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“…There exist a number of experimental techniques that have been used to study interactions between surfactants and, e.g., cell membranes [16][17][18][19]. However, obtaining a detailed understanding at the molecular level of such interactions is difficult at best.…”

Section: Introductionmentioning

confidence: 99%

Explicit-pH Coarse-Grained Molecular Dynamics Simulations Enable Insights into Restructuring of Intestinal Colloidal Aggregates with Permeation Enhancers

et al. 2021

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Permeation enhancers (PEs) can increase the bioavailability of drugs. The mechanisms of action of these PEs are complex, but, typically, when used for oral administration, they can transiently induce the alteration of trans- and paracellular pathways, including increased solubilization and membrane fluidity, or the opening of the tight junctions. To elucidate these mechanistic details, it is important to understand the aggregation behavior of not only the PEs themselves but also other molecules already present in the intestine. Aggregation processes depend critically on, among other factors, the charge state of ionizable chemical groups, which is affected by the pH of the system. In this study, we used explicit-pH coarse-grained molecular dynamics simulations to investigate the aggregation behavior and pH dependence of two commonly used PEs—caprate and SNAC—together with other components of fasted- and fed-state simulated intestinal fluids. We also present and validate a coarse-grained molecular topology for the bile salt taurocholate suitable for the Martini3 force-field. Our results indicate an increase in the number of free molecules as a function of the system pH and for each combination of FaSSIF/FeSSIF and PEs. In addition, there are differences between caprate and SNAC, which are rationalized based on their different molecular structures and critical micelle concentrations.

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TIRF Microscopy‐Based Monitoring of Drug Permeation Across a Lipid Membrane Supported on Mesoporous Silica

Cited by 7 publications

References 29 publications

Fluorescence Recovery after Photobleaching in Colloidal Science: Introduction and Application

Fluorescence Recovery after Photobleaching in Colloidal Science: Introduction and Application

Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems

Explicit-pH Coarse-Grained Molecular Dynamics Simulations Enable Insights into Restructuring of Intestinal Colloidal Aggregates with Permeation Enhancers

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