Objective: This study explored the relationships between fatigue, wellbeing and parenting in mothers of young children (aged 0-4 years) with sleep and settling difficulties. Demographic, child sleep and maternal factors associated with fatigue were also investigated. Background: Children's sleep problems can have a significant impact on parent's sleep, placing them at risk of fatigue and wellbeing difficulties. Methods: A total of 164 mothers of children aged 0-4 attending an early parenting centre completed a survey on fatigue, wellbeing and parenting. Results: Mothers reported moderate levels of fatigue, which was associated with high levels of depression, anxiety and stress. Fatigue was also significantly associated with low parental self-efficacy, parenting warmth and involvement, and high parenting hostility. Limited health and self-care behaviours, unrealistic expectations about sleep, and high need for social support were significant predictors of fatigue. Conclusion: These findings provide an empirical basis for the development of information and support for the prevention and management of parental fatigue, and have implications for professionals working with parents of children with sleep difficulties.