TIR1 auxin receptors are implicated in the differential response to 4-Cl-IAA and IAA in developing pea fruit

Jayasinghege, Charitha P. A.; Ozga, Jocelyn A.; Nadeau, Courtney; Kaur, Harleen; Reinecke, Dennis M.

doi:10.1093/jxb/ery456

Cited by 16 publications

(14 citation statements)

References 62 publications

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“…During early fruit development, an auxin gradient is established with auxin levels higher in the developing pea seeds than the surrounding pericarp tissues (Magnus et al, 1997; Figure 1a,b,f,i,l, Table S6). In the absence of developing seeds within the young pea ovary (3 DAA), auxin (IAA) levels were substantially lower in the pericarp tissue compared to pericarp tissues from ovaries with intact developing seeds (Jayasinghege, Ozga, Nadeau, Kaur, & Reinecke, 2019). Auxin‐sensitive DR5 promoter‐driven β‐glucuronidase (GUS) staining patterns localized auxin accumulation/action to the young developing seeds (at the proximal end of the seed adjacent to funiculus attachment), to the funiculus, and to the vasculature of the pericarp wall of pea (Jayasinghege et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…In the absence of developing seeds within the young pea ovary (3 DAA), auxin (IAA) levels were substantially lower in the pericarp tissue compared to pericarp tissues from ovaries with intact developing seeds (Jayasinghege, Ozga, Nadeau, Kaur, & Reinecke, 2019). Auxin‐sensitive DR5 promoter‐driven β‐glucuronidase (GUS) staining patterns localized auxin accumulation/action to the young developing seeds (at the proximal end of the seed adjacent to funiculus attachment), to the funiculus, and to the vasculature of the pericarp wall of pea (Jayasinghege et al, 2019). These results along with those from similar studies in tomato ( Solanum lycopersicum L. ; Pattison & Catalá, 2012) and strawberry ( Fragaria vesca L.; Feng et al, 2019) support the hypothesis that following fertilization, developing seeds produce auxin necessary for promoting further seed and fruit development.…”

Section: Resultsmentioning

confidence: 99%

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Balancing of hormonal biosynthesis and catabolism pathways, a strategy to ameliorate the negative effects of heat stress on reproductive growth

Kaur

Ozga

Reinecke

2020

Plant Cell & Environment

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In pea (Pisum sativum L.), moderate heat stress during early flowering/fruit set increased seed/ovule abortion, and concomitantly produced fruits with reduced ovary (pericarp) length, and fewer seeds at maturity. Plant hormonal networks coordinate seed and pericarp growth and development. To determine if these hormonal networks are modulated in response to heat stress, we analyzed the gene expression patterns and associated these patterns with precursors, and bioactive and inactive metabolites of the auxin, gibberellin (GA), abscisic acid (ABA), and ethylene biosynthesis/catabolism pathways in young developing seeds and pericarps of non-stressed and 4-day heat-stressed fruits. Our data suggest that within the developing seeds heat stress decreased bioactive GA levels reducing GA growth-related processes, and that increased ethylene levels may have promoted this inhibitory response. In contrast, heat stress increased auxin biosynthesis gene expression and auxin levels in the seeds and pericarps, and seed ABA levels, both effects can increase seed sink strength. We hypothesize that seeds with higher auxin-and ABA-induced sink strength and adequate bioactive GA levels will set and continue to grow, while the seeds with lower sink strength (low auxin, ABA, and GA levels) will become more sensitive to heat stress-induced ethylene leading to ovule/seed abortion.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Balancing of hormonal biosynthesis and catabolism pathways, a strategy to ameliorate the negative effects of heat stress on reproductive growth

Kaur

Ozga

Reinecke

2020

Plant Cell & Environment

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“…IBA does not activate the IAA signalling cascade as the molecule possesses a long side chain that makes it unable to adopt a conformation for binding to the TIR1-Aux/IAA co-receptor pocket (Uzunova et al, 2016). The auxin 4-Cl-IAA binds to the TIR1 auxin receptor (Jayasinghege et al, 2019) and is able to activate the signalling cascade with at least partial overlap in gene activation (Johnstone et al, 2005). As 4-Cl-IAA can activate the same signalling cascade as IAA it was expected that the response to the former would result in similar activation of gene transcription as that for IAA.…”

Section: Auxin Perception In Nematode Feeding Sites Through the Tir1-afb Signalling Cascadementioning

confidence: 99%

Sedentary Plant-Parasitic Nematodes Alter Auxin Homeostasis via Multiple Strategies

et al. 2021

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Sedentary endoparasites such as cyst and root-knot nematodes infect many important food crops and are major agro-economical pests worldwide. These plant-parasitic nematodes exploit endogenous molecular and physiological pathways in the roots of their host to establish unique feeding structures. These structures function as highly active transfer cells and metabolic sinks and are essential for the parasites’ growth and reproduction. Plant hormones like indole-3-acetic acid (IAA) are a fundamental component in the formation of these feeding complexes. However, their underlying molecular and biochemical mechanisms are still elusive despite recent advances in the field. This review presents a comprehensive overview of known functions of various auxins in plant-parasitic nematode infection sites, based on a systematic analysis of current literature. We evaluate multiple aspects involved in auxin homeostasis in plants, including anabolism, catabolism, transport, and signalling. From these analyses, a picture emerges that plant-parasitic nematodes have evolved multiple strategies to manipulate auxin homeostasis to establish a successful parasitic relationship with their host. Additionally, there appears to be a potential role for auxins other than IAA in plant-parasitic nematode infections that might be of interest to be further elucidated.

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“…Arabidopsis root growth responds to several auxinic compounds in addition to IAA, such as the synthetic auxins NAA and 2,4-D. Testing the effect of 4-Cl-IAA on Arabidopsis root elongation revealed it to be a more potent inhibitor than IAA [ 26 ]. Additional evidence for a potency disparity was indicated by using the synthetic auxin-signalling reporter DR5::GUS [ 27 ].…”

Section: Pod Elongation: 4-chloroindole-3-acetic Acid As a Possible Seed-to-pod Mobile Signalmentioning

confidence: 99%

Hormonal Influences on Pod–Seed Intercommunication during Pea Fruit Development

Bal

Østergaard

2021

Genes

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Angiosperms (from the Greek “angeion”—vessel, and “sperma”—seed) are defined by the presence of specialised tissue surrounding their developing seeds. This tissue is known as the ovary and once a flower has been fertilised, it gives rise to the fruit. Fruits serve various functions in relation to the seeds they contain: they often form tough physical barriers to prevent mechanical damage, they may form specialised structures that aid in dispersal, and they act as a site of nutrient and signal exchange between the parent plant and its offspring. The close coordination of fruit growth and seed development is essential to successful reproduction. Firstly, fertilisation of the ovules is required in most angiosperm species to initiate fruit growth. Secondly, it is crucial that seed dispersal facilitated by, e.g., fruit opening or ripening occurs only once the seeds have matured. These highly coordinated events suggest that seeds and fruits are in close communication throughout development and represent a classical problem of interorgan signalling and organismic resource allocation. Here, we review the contribution of studies on the edible, unicarpellate legume Pisum sativum to our understanding of seed and fruit growth coregulation, and propose areas of new research in this species which may yield important advances for both pulse agronomy and natural science.

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TIR1 auxin receptors are implicated in the differential response to 4-Cl-IAA and IAA in developing pea fruit

Abstract: The pea auxin receptors PsTIR1a, PsTIR1b, and PsAFB2 are involved in auxin perception, and the TIR1s are implicated in the differential growth response to 4-Cl-IAA and IAA in pea fruit.

Cited by 16 publications

References 62 publications

Balancing of hormonal biosynthesis and catabolism pathways, a strategy to ameliorate the negative effects of heat stress on reproductive growth

Balancing of hormonal biosynthesis and catabolism pathways, a strategy to ameliorate the negative effects of heat stress on reproductive growth

Sedentary Plant-Parasitic Nematodes Alter Auxin Homeostasis via Multiple Strategies

Hormonal Influences on Pod–Seed Intercommunication during Pea Fruit Development

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