Tip Streaming of a Lipid-Stabilized Double Emulsion Generated in a Microfluidic Channel

Torbensen, Kristian; Baroud, Charles N.; Ristori, Sandra; Abou‐Hassan, Ali

doi:10.1021/acs.langmuir.1c00827

Cited by 3 publications

(2 citation statements)

References 41 publications

(96 reference statements)

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“…To fabricate phospholipid-limited domains encapsulating the BZ reaction, DMPC (1,2-dimyristoyl- sn -glycero-3-phosphocholine) was chosen as the main component since, at room temperature, it forms fluid bilayers with high resistance to the harsh oxidizing and acidic environment, typical of the BZ reaction. − Flow-focused geometries in microfluidics allowed us to generate monodisperse micron-sized compartments by tuning the flow and other physicochemical conditions, such as lipid concentration, viscosity of the outer and inner phase, and nature of the organic layer . In this way, it was relatively simple to adjust the frequency of the compartments’ generation, their size, and the distance between different liquid–liquid interfaces …”

Section: Generation Of Compartments By Microfluidicsmentioning

confidence: 99%

“…41−43 Flow-focused geometries in microfluidics allowed us to generate monodisperse micron-sized compartments by tuning the flow and other physicochemical conditions, such as lipid concentration, viscosity of the outer and inner phase, and nature of the organic layer. 44 In this way, it was relatively simple to adjust the frequency of the compartments' generation, their size, and the distance between different liquid−liquid interfaces. 36 Our first challenge was to encapsulate the BZ reaction inside giant DMPC liposomes.…”

Section: Generation Of Compartments By Microfluidicsmentioning

confidence: 99%

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Multiscale Approach for Tuning Communication among Chemical Oscillators Confined in Biomimetic Microcompartments

Rossi,

Ristori,

Abou-Hassan

2024

Acc. Chem. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Inspired by the biological world, new cross-border disciplines and technologies have emerged. Relevant examples include systems chemistry, which offers a bottom-up approach toward chemical complexity, and bio/chemical information and communication technology (bio/chemical ICT), which explores the conditions for propagating signals among individual microreactors separated by selectively permeable membranes. To fabricate specific arrays of microreactors, microfluidics has been demonstrated as an excellent method. In particular, droplet-based microfluidics is a powerful tool for encapsulating biological entities and chemical reagents in artificial microenvironments, mostly water-in-oil microdroplets. In these systems, the interfaces are liquid−liquid, and their physicochemical properties are key factors for tuning the coupling between molecular diffusion. Simple and double emulsions, where aqueous domains are in equilibrium with oil domains through boundary layers of amphiphilic molecules, are organized assemblies with high interfacial-area-to-volume ratios. These membranes can be engineered to obtain different surface charges, single-or multilayer stacking, and a variable degree of defects in molecular packing. Emulsions find application in many fields, including the food industry, pharmaceutics, and cosmetics. Furthermore, micro-and nanoemulsions can be used to model the propagation of chemical species through long distances, which is not only vital for cell signaling but also significant in molecular computing. Here we present in-depth research on the faceted world of solutions confined in restricted environments. In particular, we focused on the multiscale aspects of structure and dynamics from molecular to micro and macro levels. The Belousov−Zhabotinsky chemical reaction, known for its robustness and well-documented oscillatory behavior, was selected to represent a generic signal emitter/receiver confined within microenvironments separated by liquid−liquid interfaces. In this pulse generator, the temporal and spatial progressions are governed by periodic fluctuations in the concentration of chemical species, which act as activatory or inhibitory messengers over long distances. When organized into "colonies" or arrays, these micro-oscillators exhibit emergent dynamical behaviors at the population level. These behaviors can be finely tuned by manipulating the geometrical distribution of the oscillators and the properties of the interfaces at the nanoscale. By carefully selecting the membrane composition, it is possible to drive the system toward either inphase, antiphase, or mixed synchronization regimes among individual oscillators, depending on messenger molecules. This relatively simple lab-scale model replicates some of the communication strategies commonly found in biological systems, particularly those based on the passive diffusion of chemical and electrical signals. It can help shed light on fundamental life processes and inspire new implementations in mo...

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Section: Generation Of Compartments By Microfluidicsmentioning

confidence: 99%

Section: Generation Of Compartments By Microfluidicsmentioning

confidence: 99%

Multiscale Approach for Tuning Communication among Chemical Oscillators Confined in Biomimetic Microcompartments

Rossi,

Ristori,

Abou-Hassan

2024

Acc. Chem. Res.

Self Cite

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Recent Advances on the Generation, Stabilization, and Potential Applications of Double Emulsions Based on the Microfluidic Strategy

Wei,

Yao,

Yue

et al. 2023

Food Eng Rev

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Stability characterization of microfluidics lipid-stabilized double emulsions under physiologically-relevant conditions

Giuliano¹,

Moran²,

Ayache³

et al. 2022

Open Res Europe

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Background: Double emulsions (DEs) are water-in-oil-in-water (or oil-in-water-in-oil) droplets with the potential to deliver combinatory therapies due to their ability to co-localize hydrophilic and hydrophobic molecules in the same carrier. However, DEs are thermodynamically unstable and only kinetically trapped. Extending this transitory state and rendering DEs more stable, would widen the possibilities of real-world applications, yet characterization of their stability in physiologically-relevant conditions is lacking. Methods: In this work, we used microfluidics to produce lipid-stabilized DEs with reproducible monodispersity and high encapsulation efficiency. We investigated DE stability under a range of physicochemical parameters such as temperature, pH and mechanical stimulus. Results: Stability through time was inversely proportional to temperature. DEs were significantly stable up to eight days at 4°C, five days at room temperature and two days at 37°C. When encapsulating a cargo, DE stability decreased significantly. When exposed to a pH change, unloaded DEs were only significantly unstable at the extremes (pH 1 and 13), largely outside physiological ranges. When exposed to flow, unloaded DEs behaved similarly regardless of the mechanical stimulus applied, with approximately 70% remaining after 100 flow cycles of 10s. Conclusions: These results indicate that lipid-stabilized DEs produced via microfluidics could be tailored to endure physiologically-relevant conditions and act as carriers for drug delivery. Special attention should be given to the composition of the solutions, e.g. osmolarity ratio between inner and outer solutions, and the interaction of the molecules, e.g. carrier and cargo, involved in the final formulation.

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Tip Streaming of a Lipid-Stabilized Double Emulsion Generated in a Microfluidic Channel

Cited by 3 publications

References 41 publications

Multiscale Approach for Tuning Communication among Chemical Oscillators Confined in Biomimetic Microcompartments

Multiscale Approach for Tuning Communication among Chemical Oscillators Confined in Biomimetic Microcompartments

Recent Advances on the Generation, Stabilization, and Potential Applications of Double Emulsions Based on the Microfluidic Strategy

Stability characterization of microfluidics lipid-stabilized double emulsions under physiologically-relevant conditions

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