TiO2, SiO2 and ZrO2 Nanoparticles Synergistically Provoke Cellular Oxidative Damage in Freshwater Microalgae

Liu, Yinghan; Wang, Se; Wang, Zhuang; Ye, Nan; Fang, Hao; Wang, Degao

doi:10.3390/nano8020095

Cited by 44 publications

(20 citation statements)

References 39 publications

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“…4a). Similarly, it was described that SiO 2 did not have any impact on membrane integrity of algal cells Scenedesmus obliquus (Liu et al 2018). Although it was reported the disruption of cell membrane in yeasts exposed to Al 2 O 3 NPs (Garcia-Saucedo et al 2011) or Mn 2 O 3 (Otero-Gonzalez et al 2013), both results were observed in the presence of 1 g/L NPs, which corresponded to a concentration ten times higher than the one used in the present study.…”

Section: Discussionsupporting

confidence: 64%

Metal(loid) oxide (Al2O3, Mn3O4, SiO2 and SnO2) nanoparticles cause cytotoxicity in yeast via intracellular generation of reactive oxygen species

Sousa

Soares

2019

Appl Microbiol Biotechnol

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In this work, the physicochemical characterization of five (Al 2 O 3 , In 2 O 3 , Mn 3 O 4 , SiO 2 and SnO 2) nanoparticles (NPs) was carried out. In addition, the evaluation of the possible toxic impacts of these NPs and the respective modes of action were performed using the yeast Saccharomyces cerevisiae. In general, in aqueous suspension, metal(loid) oxide (MOx) NPs displayed an overall negative charge and agglomerated; these NPs were practically insoluble (dissolution < 8%) and did not generate detectable amounts of reactive oxygen species (ROS) under abiotic conditions. Except In 2 O 3 NPs, which did not induce an obvious toxic effect on yeast cells (up to 100 mg/L), the other NPs induced a loss of cell viability in a dose-dependent manner. The comparative analysis of the loss of cell viability induced by the NPs with the ions released by NPs (NPs supernatant) suggested that SiO 2 toxicity was mainly caused by the NPs themselves, Al 2 O 3 and SnO 2 toxic effects could be attributed to both the NPs and the respective released ions and Mn 3 O 4 harmfulness could be mainly due to the released ions. Al 2 O 3 , Mn 3 O 4 , SiO 2 and SnO 2 NPs induced the loss of metabolic activity and the generation of intracellular ROS without permeabilization of plasma membrane. The co-incubation of yeast cells with MOx NPs and a free radical scavenger (ascorbic acid) quenched intracellular ROS and significantly restored cell viability and metabolic activity. These results evidenced that the intracellular generation of ROS constituted the main cause of the cytotoxicity exhibited by yeasts treated with the MOx NPs. This study highlights the importance of a ROS-mediated mechanism in the toxicity induced by MOx NPs.

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Section: Discussionsupporting

confidence: 64%

Metal(loid) oxide (Al2O3, Mn3O4, SiO2 and SnO2) nanoparticles cause cytotoxicity in yeast via intracellular generation of reactive oxygen species

Sousa

Soares

2019

Appl Microbiol Biotechnol

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“…3B). A similar result was observed with the alga Scenedesmus obliquus when exposed to SiO 2 NPs (Liu et al, 2018). In relation to protein oxidation, different mechanisms were described, which includes the oxidation of amino acid residues containing aromatic side chain or sulfhydryl groups (Cecarini et al, 2007).…”

Section: )supporting

confidence: 74%

Chronic exposure of the freshwater alga Pseudokirchneriella subcapitata to five oxide nanoparticles: Hazard assessment and cytotoxicity mechanisms

Sousa

Soares

2019

Aquatic Toxicology

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The increasing use of nanoparticles (NPs) unavoidably enhances their unintended introduction into the aquatic systems, raising concerns about their nanosafety. This work aims to assess the toxicity of five oxide NPs (Al 2 O 3 , Mn 3 O 4 , In 2 O 3 , SiO 2 and SnO 2) using the freshwater alga Pseudokirchneriella subcapitata as a primary producer of ecological relevance. These NPs, in OECD medium, were poorly soluble and unstable (displayed low zeta potential values and presented the tendency to agglomerate). Using the algal growth inhibition assay and taking into account the respective 72 h-EC 50 values, it was possible to categorize the NPs as: toxic (Al 2 O 3 and SnO 2); harmful (Mn 3 O 4 and SiO 2) and non-toxic/non-classified (In 2 O 3). The toxic effects were mainly due to the NPs, except for SnO 2 which toxicity can mainly be attributed to the Sn ions leached from the NPs. A mechanistic study was undertaken using different physiological endpoints (cell membrane integrity, metabolic activity, photosynthetic efficiency and intracellular ROS accumulation). It was observed that Al 2 O 3, Mn 3 O 4 and SiO 2 induced an algistatic effect (growth inhibition without loss of membrane integrity) most likely as a consequence of the cumulative effect of adverse outcomes: i) reduction of the photosynthetic efficiency of the photosystem II (Ф PSII); ii) intracellular ROS accumulation and iii) loss of metabolic activity. SnO 2 NPs also provoked an algistatic effect probably as a consequence of the reduction of Ф PSII since no modification of intracellular ROS levels and metabolic activity were observed. Altogether, the results here presented allowed to categorize the toxicity of the five NPs and shed light on the mechanisms behind NPs toxicity in the green alga P. subcapitata.

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“…Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and peroxidase (POD), among other enzymes, are mechanisms of antioxidant protection in algal cells. In the presence of less toxic NPs, the activity of these antioxidant enzymes increases to eliminate the toxicity of ROS to algal cells [63], and when the toxicity of NPs in water is too strong, the antioxidant defense system of algae cells is destroyed and the enzyme activity is significantly inhibited [45,64].…”

Section: Effect Of Nps On Intracellular Substratesmentioning

confidence: 99%

Effects of Nanoparticles on Algae: Adsorption, Distribution, Ecotoxicity and Fate

et al. 2019

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With the rapid development of nanotechnology and widespread use of nanoproducts, the ecotoxicity of nanoparticles (NPs) and their potential hazards to the environment have aroused great concern. Nanoparticles have increasingly been released into aquatic environments through various means, accumulating in aquatic organisms through food chains and leading to toxic effects on aquatic organisms. Nanoparticles are mainly classified into nano-metal, nano-oxide, carbon nanomaterials and quantum dots according to their components. Different NPs may have different levels of toxicity and effects on various aquatic organisms. In this paper, algae are used as model organisms to review the adsorption and distribution of NPs to algal cells, as well as the ecotoxicity of NPs on algae and fate in a water environment, systematically. Meanwhile, the toxic effects of NPs on algae are discussed with emphasis on three aspect effects on the cell membrane, cell metabolism and the photosynthesis system. Furthermore, suggestions and prospects are provided for future studies in this area.

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TiO2, SiO2 and ZrO2 Nanoparticles Synergistically Provoke Cellular Oxidative Damage in Freshwater Microalgae

Cited by 44 publications

References 39 publications

Metal(loid) oxide (Al2O3, Mn3O4, SiO2 and SnO2) nanoparticles cause cytotoxicity in yeast via intracellular generation of reactive oxygen species

Metal(loid) oxide (Al2O3, Mn3O4, SiO2 and SnO2) nanoparticles cause cytotoxicity in yeast via intracellular generation of reactive oxygen species

Chronic exposure of the freshwater alga Pseudokirchneriella subcapitata to five oxide nanoparticles: Hazard assessment and cytotoxicity mechanisms

Effects of Nanoparticles on Algae: Adsorption, Distribution, Ecotoxicity and Fate

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