TiO2 nanoparticles affect spermatogenesis and adhesion junctions via the ROS-mediated mTOR signalling pathway in Eriocheir sinensis testes

Wang, Lan-Min; Jia, Ke; Li, Zhenfang; Qi, Hongyu; Dingxi, Liu; Liang, Yixiao; Hao, Shuang-Li; Tan, Fu-Qing; Yang, Wan-Xi

doi:10.1016/j.envpol.2023.121952

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…Although there are few studies on the toxicity of 2D-like materials [ 18 ], except for graphene and graphene oxide, which are the most widely studied [ 19 ], the scientific literature abounds on the consequences of the toxicity of nanoparticles (NPs) [ 20 , 21 ]. For example, titanium dioxide (TiO 2 ) NPs have shown delayed reproduction in aquatic organisms [ 22 , 23 ], cerium dioxide (CeO 2 ) NPs have generated genotoxicological damage in the DNA strands of D. magna and C. riparius [ 24 ], and phosphorus has induced both genotoxic and morphological damage in zebrafish [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Ecotoxicological Properties of Pure and Phosphorus-Containing Graphene Oxide Bidimensional Sheets in Daphnia magna

Mendoza-Villa,

Checca-Huaman,

Costa

et al. 2024

Toxics

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In this work, the synthesis and structural, thermal, vibrational, morphological, and electronic characterization of 2D-like pure graphene oxide (GO) and phosphorus-containing graphene oxide (GOP) sheets were investigated. The average thicknesses of GO and GOP were 0.8 μm and 3.1 μm, respectively. The electron energy-loss spectroscopy spectra were used to analyze the differences in the C-K and O-K energy edge bands between GO and GOP. In addition, colloidal stability was studied using dynamic light scattering and zeta potential physicochemical techniques, determining that as the concentration increases, the hydrodynamic diameter and electrostatic stability of GO and GOP increase. The colloidal stability was quite important to ensure the interaction between the suspended solid phase and the biomarker. The 2D-like materials were used to determine their ecotoxicological properties, such as the medium lethal concentration, a crucial parameter for understanding ecotoxicity. Acute ecotoxicity experiments (24 h) were conducted in triplicate to obtain robust statistics, with corresponding mean lethal concentration (LC50) of 11.4 mg L−1 and 9.8 mg L−1 for GO and GOP, respectively. The morphological parameters of GO and GOP were compared with a negative control. However, only the case of GO was analyzed, since the Daphnia magna (D. magna) set exposed to GOP died before completing the time required for morphological analysis. The results indicate that the GOP sample is more toxic than the GO, both during and after exposure. Furthermore, the morphological parameters with the greatest statistically significant changes (p<0.05) were associated with the heart and body, while the eye and tail showed less significant changes.

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Section: Introductionmentioning

confidence: 99%

Ecotoxicological Properties of Pure and Phosphorus-Containing Graphene Oxide Bidimensional Sheets in Daphnia magna

Mendoza-Villa,

Checca-Huaman,

Costa

et al. 2024

Toxics

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“…It was reported that TiO 2 NPs led to endothelial cell apoptosis via modulation of the p38 signaling (Gholinejad et al, 2019). Additionally, TiO 2 NPs induced apoptosis in germ cells of Eriocheir sinensis testes, implicating the involvement of the AKT/mTOR pathway (Wang et al, 2023). Indeed, p38 can regulate AKT activity and plays a crucial role in AKT phosphorylation (Rane et al, 2001).…”

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confidence: 98%

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells

Wang,

Yang,

et al. 2024

J of Applied Toxicology

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Titanium dioxide nanoparticles (TiO2 NPs) can cause apoptosis in TM4 cells; however, the underlying mechanism has not been entirely elucidated. The purpose of this study was to investigate the effects of TiO2 NPs on ROS, Ca2+ level, p38/AKT/mTOR pathway, and apoptosis in TM4 cells and to evaluate the role of Ca2+ in p38/AKT/mTOR pathway and apoptosis. After exposure to different concentrations (0, 50, 100, 150, and 200 μg/mL) of TiO2 NPs for 24 h, cell viability, ROS, Ca2+ level, Ca2+‐ATPase activity, p38/AKT/mTOR pathway‐related proteins, apoptosis rate, and apoptosis‐related proteins (Bax, Bcl‐2, Caspase 3, Caspase 9, and p53) were detected. The ROS scavenger NAC was used to determine the effect of ROS on Ca2+ level. The Ca2+ chelator BAPTA‐AM was used to evaluate the role of Ca2+ in p38/AKT/mTOR pathway and apoptosis. TiO2 NPs significantly inhibited cell viability, increased ROS level, and elevated Ca2+ level while suppressing Ca2+‐ATPase activity. TiO2 NPs regulated the p38/AKT/mTOR pathway via increasing p‐p38 level and decreasing p‐AKT and p‐mTOR levels. TiO2 NPs significantly enhanced the apoptosis. NAC attenuated Ca2+ overload and reduction in Ca2+‐ATPase activity caused by TiO2 NPs. BAPTA‐AM alleviated TiO2 NPs‐induced abnormal expression of p38/AKT/mTOR pathway‐related proteins. BAPTA‐AM assuaged the apoptosis caused by TiO2 NPs. Altogether, this study revealed that TiO2 NPs elevated intracellular Ca2+ level through ROS accumulation. Subsequently, the heightened intracellular Ca2+ level was observed to exert regulation over the p38/AKT/mTOR pathway, ultimately culminating in apoptosis. These results provides a complementary understanding to the mechanism of TiO2 NPs‐induced apoptosis in TM4 cells.

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The ROS Mediates MCUb in Mitochondria-Regulated Apoptosis of TM4 Cells Induced by Titanium Dioxide Nanoparticles

Sun,

Wang,

et al. 2024

Biol Trace Elem Res

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TiO2 nanoparticles affect spermatogenesis and adhesion junctions via the ROS-mediated mTOR signalling pathway in Eriocheir sinensis testes

Cited by 4 publications

References 47 publications

Ecotoxicological Properties of Pure and Phosphorus-Containing Graphene Oxide Bidimensional Sheets in Daphnia magna

Ecotoxicological Properties of Pure and Phosphorus-Containing Graphene Oxide Bidimensional Sheets in Daphnia magna

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells

The ROS Mediates MCUb in Mitochondria-Regulated Apoptosis of TM4 Cells Induced by Titanium Dioxide Nanoparticles

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TiO2 nanoparticles affect spermatogenesis and adhesion junctions via the ROS-mediated mTOR signalling pathway in Eriocheir sinensis testes

Cited by 4 publications

References 47 publications

Ecotoxicological Properties of Pure and Phosphorus-Containing Graphene Oxide Bidimensional Sheets in Daphnia magna

Ecotoxicological Properties of Pure and Phosphorus-Containing Graphene Oxide Bidimensional Sheets in Daphnia magna

Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca2+‐p38/AKT/mTOR pathway in TM4 cells

The ROS Mediates MCUb in Mitochondria-Regulated Apoptosis of TM4 Cells Induced by Titanium Dioxide Nanoparticles

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Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells