Tiny percutaneous needle biopsy: An efficient method for studying cellular and molecular aspects of skeletal muscle in humans

Pietrangelo, Tiziana; D’Amelio, L.; Doria, Christian; Mancinelli, Rosa; Fulle, Stefania; Fanò, Giorgio

doi:10.3892/ijmm.2010.582

Cited by 25 publications

(26 citation statements)

References 22 publications

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“…Human muscle samples were derived from Vastus Lateralis muscle biopsies using the tiny percutaneous needle biopsy (TPNB) (Pietrangelo et al., ). Young and elderly healthy untrained subjects underwent voluntary biopsies.…”

Section: Methodsmentioning

confidence: 99%

Aging affects the in vivo regenerative potential of human mesoangioblasts

et al. 2018

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SummarySarcopenia is the age‐related loss of muscle mass, strength, and function. Although the role of human satellite cells (SCs) as adult skeletal muscle stem cells has been deeply investigated, little is known about the impact of aging on muscle interstitial stem cells. Here, we isolated the non‐SC CD56– fraction from human muscle biopsies of young and elderly subjects. The elderly interstitial cell population contained a higher number of CD15+ and PDGFRα+ cells when compared to young samples. In addition, we found that the CD56–/ALP + cells were well represented as a multipotent stem cell population inside the CD56– fraction. CD56–/ALP +/CD15– cells were clonogenic, and since they were myogenic and expressed NG2, α‐SMA and PDGFRβ can be considered mesoangioblasts (MABs). Interestingly, elderly MABs displayed a dramatic impairment in the myogenic differentiation ability in vitro and when transplanted in dystrophic immunodeficient Sgcb‐null Rag2‐null γc‐null mice. In addition, elderly MABs proliferated less, but yet retained other multilineage capabilities. Overall, our results indicate that aging negatively impacted on the regenerative potential of MABs and this should be carefully considered for potential therapeutic applications of MABs.

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Section: Methodsmentioning

confidence: 99%

Aging affects the in vivo regenerative potential of human mesoangioblasts

et al. 2018

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“…d′Annunzio' University of Chieti–Pescara (protocol numbers: 1233/06 COET, dated 25 July 2006; 1884 COET, dated 15 May 2009; and 1634/08 COET, dated 24 June 2008). 39 The inclusion criteria were as follows: normal ECG and blood pressure; lack of bone and joint disorders, or metabolic (that is, diabetes) and/or cardiovascular diseases. The exclusion criteria were as follows: the presence of metabolic and/or cardiovascular diseases, evidence of hereditary or acquired muscular disorders, diagnosis of respiratory disorders or psychiatric problems.…”

Section: Methodsmentioning

confidence: 99%

Dual role of the caspase enzymes in satellite cells from aged and young subjects

et al. 2013

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Satellite cell (SC) proliferation and differentiation have critical roles in skeletal muscle recovery after injury and adaptation in response to hypertrophic stimuli. Normal ageing hinders SC proliferation and differentiation, and is associated with increased expression of a number of pro-apoptotic factors in skeletal muscle. In light of previous studies that have demonstrated age-related altered expression of genes involved in SC antioxidant and repair activity, this investigation was aimed at evaluating the incidence of apoptotic features in human SCs. Primary cells were obtained from vastus lateralis of nine young (27.3±2.0 years old) and nine old (71.1±1.8 years old) subjects, and cultured in complete medium for analyses at 4, 24, 48, and 72 h. Apoptosis was assessed using AnnexinV/propidium iodide staining, the terminal deoxynucleotidyl transferase dUTP nick-end labelling technique, RT-PCR, DNA microarrays, flow cytometry, and immunofluorescence analysis. There was an increased rate of apoptotic cells in aged subjects at all of the experimental time points, with no direct correlation between AnnexinV-positive cells and caspase-8 activity. On the other hand, CASP2, CASP6, CASP7, and CASP9 and a number of cell death genes were upregulated in the aged SCs. Altogether, our data show age-related enhanced susceptibility of human SCs to apoptosis, which might be responsible for their reduced response to muscle damage.

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“…We further obtained three small biopsy samples from the vastus lateralis (VL) muscle from 32–66-year-old male healthy volunteers (indicated as M32, 32 years old; M33, 41 years old; M34, 66 years old, respectively) using the TPNB procedure as described in our previous study [Pietrangelo et al (1)]. The study was approved by the Ethics Committee of the University of Chieti-Pescara (approval protocol no.…”

Section: Methodsmentioning

confidence: 99%

“…We recently described a procedure for human muscle biopsy that we termed tiny percutaneous needle biopsy (TPNB) and we demonstrated that it is an excellent method for obtaining human skeletal muscle specimens with the least trauma for the patient (1). The classically and commonly used needle biopsy technique is needle aspiration biopsy (NAB), using a Bergstrom needle (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…Similar to NAB, TPNB involves a percutaneous approach (thus no need for an invasive skin incision) and the use of a penetrating needle. However, TPNB differs significantly in the considerably smaller size of the sample and in the more automated and rapid penetration of the needle into the depths of the muscle, resulting in a considerably less traumatic approach and relatively rapid and orderly regeneration of the tissue (1). To support the low invasiveness of TPNB, we previously analyzed nuclear magnetic resonance (NMR) images of muscles following TPNB (unpublished data).…”

Section: Introductionmentioning

confidence: 99%

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A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle

Pietrangelo

Perni

Tano

et al. 2013

International Journal of Molecular Medicine

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Skeletal muscle biopsies require transecting the muscle fibers resulting, in structural damage near the cut ends. Classically, the optimal ultrastructural preservation has been obtained by the use of relatively large biopsies in which the tissue fibers are restrained by ligating to a suitable retaining support prior to excision, and by examining regions at some distance from the cut ends. However, these methods require invasive surgical procedures. In the present study, we present and substantiate an alternative approach that allows for the excellent ultrastructural preservation of needle biopsy samples, even the very small samples obtained through tiny percutaneous needle biopsy (TPNB). TPNB represents an advantage, relative to standard muscle biopsy techniques and to other needle biopsies currently in use, as in addition to not requiring a skin incision, it leaves no scars in the muscle and requires an extremely brief recovery period. It is most appropriate for obtaining repeated samples in horizontal studies, e.g., in order to follow changes with athletic training and/or aging in a single individual and for studies of sarcopenic muscles in elderly patients. Due to the small size of the sample, TPNB may present limited usefulness for classical pathology diagnostics. However, it offers the major advantage of allowing multiple samples within a single session and this may be useful under specific circumstances.

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Tiny percutaneous needle biopsy: An efficient method for studying cellular and molecular aspects of skeletal muscle in humans

Cited by 25 publications

References 22 publications

Aging affects the in vivo regenerative potential of human mesoangioblasts

Aging affects the in vivo regenerative potential of human mesoangioblasts

Dual role of the caspase enzymes in satellite cells from aged and young subjects

A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle

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