The high demand for meat makes the government have to carry out self-sufficiency in meat. Efforts to increase and improve cattle population can be done by increasing the use of Artificial Insemination (AI) technology, Embryo Tansfer (ET) and optimizing natural mating. This study aimed to determine and observe the effect of estrous synchronization using Ovsyhnc method on estrous response and conception rate in PO cattle's Belgian Blue acceptors. The study was conducted for 4 months, in April until July 2019, in Beef Cattle Research Station, Grati, Pasuruan, East Java. About 17 PO cows were given Ovsynch treatment with 2 (two) GnRh injections and 1 (one) injection of PGF2alfa. The order of the Ovsynch method was on day 0, injected GnRH (Fertagyl) 2.5 cc intramuscular, on day 7 inject PGF2Alfa 5cc intramuscular, on day 9 again inject GnRH (Fertagyl) 2.5 cc intramuscular. AI was done after the cow showed signs of estrous. The results showed that the estrous response shown by Ovsynh method PO cattle was 76.47% and the pregnancy rate obtained was 23,07%. The study concluded that the Ovsynch method showed a moderately estrous response but the percentage of pregnancy results of the artificial insemination of BB was relatively moderate.