Tingginya Prevalensi MRSA pada Isolat Klinik Periode 2010- 2014 di RSUD Dr. Saiful Anwar Malang, Indonesia

Erikawati, Dewi; Santosaningsih, Dewi; Santoso, Sanarto

doi:10.21776/ub.jkb.2016.029.02.9

Cited by 8 publications

(5 citation statements)

References 5 publications

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“…20 A study conducted at Dr. Saiful Anwar Hospital found that predominant isolates of MRSA from pus were 49%, this results similar to this study. 21 This study carried out the polymerase chain reaction on 23 MRSA isolates. It was found that 17 isolates (73.91%) had SCCmec type III and pvl genes.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of SCCmec Types I, II, III, and pvl gene among Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from clinical specimens in Sanglah General Hospital

Agastya¹,

Darwinata²,

Pinatih³

et al. 2022

Intisari Sains Medis

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Background: Methicillin-Resistant Staphylococcus aureus (MRSA) is a big challenge for health services worldwide which causes infections both in healthcare and community. Healthcare-associated MRSA (HA-MRSA) strains are shown to be resistant to beta-lactam antibiotics and several non-beta lactam antibiotics. At the same time, the community-associated MRSA (CA-MRSA) tends to be resistant to beta-lactam antibiotics. MRSA carried staphylococcal cassette chromosome (SCCmec) types I, II, III, IV, and V. SCCmec types I, II, and III were predominantly found in HA-MRSA strain while SCCmec types IV and V predominantly found in CA-MRSA strains. Furthermore, the panton valentine leukocidine (pvl) gene is commonly found in CA-MRSA strains. Therefore, this study aimed to determine the prevalence of SCCmec types I, II, III, and pvl gene in MRSA isolated from clinical specimens in Sanglah General Hospital. Methods: This study was a cross-sectional descriptive study. MRSA was isolated from clinical specimens (sputum, wounds, tissue, blood, etc.) from January 2020 to July 2021 and identified by the Vitek 2 Compact (Biomerieux, France) at the Clinical Microbiology Laboratory of Sanglah Hospital. Prevalence of SCCmec and pvl gene using PCR. Data were analyzed using Microsoft Excel version 2010 for Windows. Results: Most of the specimens (69.56%) were wound. Seventeen (73.91%) out of 23 MRSA isolates were positive for the SCCmec III and pvl gene, while none was positive for the SCCmec I and SCCmec II. About 19 (82.60%) isolates were resistant to two or more non-beta-lactam antibiotics. Conclusions: The isolates of MRSA in this study were predominantly isolated from wound specimens, with the most prevalent genetic element being SCCmec III. In this study, although most MRSA isolates carried SCCmec III that suggested as HA-MRSA, however, most of the strains harbored the pvl gene. This interesting phenomenon needs to be further elucidated.

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Section: Methodsmentioning

confidence: 99%

Prevalence of SCCmec Types I, II, III, and pvl gene among Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from clinical specimens in Sanglah General Hospital

Agastya¹,

Darwinata²,

Pinatih³

et al. 2022

Intisari Sains Medis

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“…Salah satu patogen resisten adalah MRSA (Methicillin-resistant Staphylococcus aureus). MRSA merupakan patogen nosokomial yang dapat menyebabkan penyakit infeksi kulit dan jaringan lunak, seperti impetigo, endokarditis, bahkan sepsis (Erikawati et al, 2016;Grema, 2015). WHO (World Health Organization) melaporkan bahwa MRSA termasuk dalam prioritas tinggi patogen resisten yang harus diatasi dengan menggunakan agen antibakteri baru (WHO, 2017).…”

Section: Pendahuluanunclassified

Perbandingan Kandungan Senyawa Kimia dan Aktivitas Antibakteri terhadap MRSA (Methicillin-resistant Staphylococcus aureus) Beberapa Minyak Atsiri Daun Salam (Syzygium polyanthum)

et al. 2019

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Daun Syzygium polyanthum secara tradisional telah digunakan untuk mengobati penyakit yang disebabkan oleh bakteri. Kandungan kimia dari minyak atsri dari suatu tanaman dipengaruhi oleh daerah asal tumbuhnya. Tujuan penelitian ini untuk mengetahui komponen minyak atsiri daun S. polyanthum dari Bekasi, Lembang, Purwokerto Baturaden dan Curup, serta aktivitas antibakterinya terhadap MRSA Minyak atsiri daun S. polyanthum diperoleh dengan metode destilasi uap. Kandungan komponen senyawa pada minyak atsiri dianalisis menggunakan GC-MS. Sifat antibakteri minyak atisri dievaluasi menggunakan metode difusi cakram pada rentang konsentrasi 12 ̶ 100 ppm. Rendemen minyak atsiri daun S. polyanthum yang diperoleh dari Bekasi, Lembang, Purwokerto Baturaden dan Curup berturut-turut sebesar 0,03%; 0,06%; 0,072%; 0,120% dan 0,067%. Hasil analisis GC-MS komponen minyak atsiri daun S. polyanthum dari berbagai daerah tersebut memperlihatkan senyawa aldehid dan terpenoid sebagai komponen utama. Minyak atsiri daun S. polyanthum dari Bekasi, Purwokerto, Baturaden dan Curup dapat memberikan daya hambat terhadap MRSA sebesar 9,2 ̶ 15,3 mm, sedangkan minyak atsiri dari Lembang tidak memberikan daya hambat. Minyak atsiri daun S. polyanthum daerah Baturaden memiliki aktivitas menghambat pertumbuhan bakteri MRSA lebih baik dibandingkan daerah lainnya yaitu 15,3 mm pada konsentrasi 100 ppm dengan kategori intermediet. Penelitian mengenai perbandingan komponen kimia dan sifat antibakteri MRSA minyak atsiri daun S. polyanthum dari berbagai daerah ini baru pertama kali dilaporkan.

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“…One of the causes of infectious diseases is bacteria. S. aureus is capable of causing various infections with different levels of severity in soft tissue, bone tissue, respiratory organs, and endovascular tissue (Erikawati et al, 2016). Meanwhile, E. coli bacteria commonly cause diarrhea, urinary tract infections, pneumonia, wound infections, especially in the abdomen, and meningitis (Suryati et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Namnam Fruit Juice From South Kalimantan Against Staphylococcus Aureus and Escherichia Coli

Nurbidayah¹,

Nafila²,

Amalia³

2023

J Vocat Heal Stud

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Background: Namnam (Cynometra cauliflora L.) has been reported to contain bioactive compounds that are beneficial as being antifungal, antibacterial, antiviral, and antioxidant. Namnam fruit (Cynometra cauliflora L.) is a herbal plant that has antibacterial properties because it contains chemicals in the form of tannins, saponins, and flavonoids. Purpose: To determine the content of phytochemical compounds contained in them and their activity against Staphylococcus aureus and Eshcerichia coli. Method: The method used in this study was agar diffusion using blank disks and the measurement of the inhibition of the test bacteria. Result: The results showed that Namnam fruit juice (Cynometra cauliflora L.) was proven to contain alkaloids, phenol, flavonoid, and saponin phytochemicals. The results of the inhibition zone measurement for S. aureus bacteria at a 100% concentration showed 6 mm and for E. coli bacteria at a 100% concentration showed 7.33 mm, which means that the inhibition zone of the two bacteria was quite intense. It meant that the higher the concentration, the greater the inhibition zone and the stronger the antibacterial activity produced. Namnam fruit juice activity in inhibiting the growth of E. coli bacteria was more substantial when compared to S. aureus bacteria. Conclusion: The result of this study concluded that Namnam fruit juice had the potential to be an antibacterial agent.

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Tingginya Prevalensi MRSA pada Isolat Klinik Periode 2010- 2014 di RSUD Dr. Saiful Anwar Malang, Indonesia

Cited by 8 publications

References 5 publications

Prevalence of SCCmec Types I, II, III, and pvl gene among Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from clinical specimens in Sanglah General Hospital

Prevalence of SCCmec Types I, II, III, and pvl gene among Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from clinical specimens in Sanglah General Hospital

Perbandingan Kandungan Senyawa Kimia dan Aktivitas Antibakteri terhadap MRSA (Methicillin-resistant Staphylococcus aureus) Beberapa Minyak Atsiri Daun Salam (Syzygium polyanthum)

Antibacterial Activity of Namnam Fruit Juice From South Kalimantan Against Staphylococcus Aureus and Escherichia Coli

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