TIMP1 in conditioned media of human adipose stromal cells protects neurons against oxygen-glucose deprivation injury

Du, Shiwei; Mao, Gengsheng; Zhu, Timothy; Luan, Zuo; Du, Yansheng; Gu, Huiying

doi:10.1016/j.neulet.2014.09.045

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…To neutralize the activity of NGF, a polyclonal anti-NGF antibody (Abcam, Cambridge, MA, USA) was introduced and tested for its specificity. No cross-reactivity has been detected after using it against VEGF, GDNF, IGF1 and BDNF.1 μg/μl of the antibody was incubated with ASC-CM at 5 μg/ml overnight at 4 °C 28 37 . NGF antibody neutralized ASC-CM treated mice group (ASC-CM-NGF Ab) received 200 ul ASC-CM-NGF antibody once daily.…”

Section: Methodsmentioning

confidence: 99%

Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mouse model of Amyotrophic Lateral Sclerosis

Fontanilla

Liu

et al. 2015

Sci Rep

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Adipose stromal cells (ASC) secrete various trophic factors that assist in the protection of neurons in a variety of neuronal death models. In this study, we tested the effects of human ASC conditional medium (ASC-CM) in human amyotrophic lateral sclerosis (ALS) transgenic mouse model expressing mutant superoxide dismutase (SOD1G93A). Treating symptomatic SOD1G93A mice with ASC-CM significantly increased post-onset survival time and lifespan. Moreover, SOD1G93A mice given ASC-CM treatment showed high motor neuron counts, less activation of microglia and astrocytes at an early symptomatic stage in the spinal cords under immunohistochemical analysis. SOD1G93A mice treated with ASC-CM for 7 days showed reduced levels of phosphorylated p38 (pp38) in the spinal cord, a mitogen-activated protein kinase that is involved in both inflammation and neuronal death. Additionally, the levels of α-II spectrin in spinal cords were also inhibited in SOD1G93A mice treated with ASC-CM for 3 days. Interestingly, nerve growth factor (NGF), a neurotrophic factor found in ASC-CM, played a significant role in the protection of neurodegeneration inSOD1G93A mouse. These results indicate that ASC-CM has the potential to develop into a novel and effective therapeutic treatment for ALS.

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Section: Methodsmentioning

confidence: 99%

Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mouse model of Amyotrophic Lateral Sclerosis

Fontanilla

Liu

et al. 2015

Sci Rep

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“…Fibronectins promote cell motility, adhesion, and increased wound healing [ 41 ]. TIMP-1 inhibits metalloproteinase irreversibly by binding to its catalytic zinc cofactor [ 42 ]. SERPINE-1 is a type of serine protease inhibitor [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging

Guo

Cui³

et al. 2016

Stem Cells International

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Background. Mesenchymal stem cells are the most commonly used seed cells in biomedical research and tissue engineering. Their secretory proteins have also been proven to play an important role in tissue healing. Methods. We isolated adipose stem cells and placental stem cells and performed analysis examining characteristics. The secretory proteins were extracted from conditioned medium and analyzed by MALDI-TOF/TOF. The antiaging effect of conditioned mediums was evaluated by the results of facial skin application. Results. Adipose stem cells and placental stem cells were found to be very similar in their surface markers and multipotency. The specific proteins secreted from adipose stem cells were more adept at cell adhesion, migration, wound healing, and tissue remodeling, while the proteins secreted by placental stem cells were more adept at angiogenesis, cell proliferation, differentiation, cell survival, immunomodulation, and collagen degradation. While these two types of conditioned medium could improve the facial index, the improvement of Melanin index after injection of the adipose stem cell conditioned medium was much more significant. Conclusion. The results suggest that the secreted proteins are ideal cell-free substances for regeneration medicine, especially in the antiaging field.

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“…When the density of RGC-5 cells was around 70%, cells were washed twice with glucose-free DMEM (Sigma-Aldrich), then placed in the same medium in an anaerobic chamber with 95% N 2 and 5% CO 2 for 8 h at 37°C to induce OGD [ 19 , 20 ]. After 8 h, OGD was terminated by removing the cell culture flasks from the anoxic chamber and replacing the glucose-free DMEM with regular culture medium.…”

Section: Methodsmentioning

confidence: 99%

Receptor interacting protein 3-induced RGC-5 cell necroptosis following oxygen glucose deprivation

et al. 2015

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BackgroundNecroptosis is a type of regulated form of cell death that has been implicated in the pathogenesis of various diseases. Receptor-interacting protein 3 (RIP3), a member of the RIP family of proteins, has been reported as an important necroptotic pathway mediator in regulating a variety of human diseases, such as myocardial ischemia, inflammatory bowel disease, and ischemic brain injury. Our previous study showed that RIP3 was expressed in rat retinal ganglion cells (RGCs), where it was significantly upregulated during the early stage of acute high intraocular pressure. Furthermore, RIP3 expression was co-localized with propidium iodide (PI)-positive staining (necrotic cells). These results suggested that RIP3 up-regulation might be involved in the necrosis of injured RGCs. In this study, we aimed to reveal the possible involvement of RIP3 in oxygen glucose deprivation (OGD)-induced retinal ganglion cell-5 (RGC-5) necroptosis.MethodsRGC-5 cells were cultured in Dulbecco’s-modified essential medium and necroptosis was induced by 8 h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. RIP3 expression was detected by western blot and flow cytometry was used to detect the effect of RIP3 on RGC-5 necroptosis following OGD in rip3 knockdown cells. Malondialdehyde (MDA) lipid peroxidation assay was performed to determine the degree of oxidative stress.ResultsPI staining showed that necrosis was present in the early stage of OGD-induced RGC-5 cell death. The presence of RGC-5 necroptosis after OGD was detected by flow cytometry using necrostatin-1, a necroptosis inhibitor. Western blot demonstrated that RIP3 up-regulation may be involved in RGC-5 necroptosis. Flow cytometry revealed that the number of OGD-induced necrotic RGC-5 cells was reduced after rip3 knockdown. Furthermore, MDA levels in the normal RGC-5 cells were much higher than in the rip3-knockdown cells after OGD.ConclusionsOur findings suggest that RGC-5 cell necroptosis following OGD is mediated by a RIP3-induced increase in oxidative stress.

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TIMP1 in conditioned media of human adipose stromal cells protects neurons against oxygen-glucose deprivation injury

Cited by 9 publications

References 37 publications

Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mouse model of Amyotrophic Lateral Sclerosis

Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mouse model of Amyotrophic Lateral Sclerosis

The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging

Receptor interacting protein 3-induced RGC-5 cell necroptosis following oxygen glucose deprivation

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