TIMM13 as a prognostic biomarker and associated with immune infiltration in skin cutaneous melanoma (SKCM)

Abstract

ObjectiveProviding protection against aggregation and guiding hydrophobic precursors through the mitochondria’s intermembrane space, this protein functions as a chaperone-like protein. SLC25A12 is imported by TIMM8 as a result of its interaction with TIMM13. In spite of this, it is still unknown how TIMM13 interacts with skin cutaneous melanoma (SKCM) and tumor-infiltrating lymphocytes (TILs).MethodsAberrant expression of TIMM13 in SKCM and its clinical outcome was evaluated with the help of multiple databases, including the Xiantao tool (https://www.xiantao.love/), HPA, and UALCAN. TISIDB and Tumor Immune Estimation Resources (TIMER) databases were applied to explore the association between TIMM13 and tumor infiltration immune cells. OS nomogram was constructed, and model performance was examined. Finally, TIMM13 protein expression was validated by immunohistochemistry (IHC).ResultsTIMM13 expression was higher in SKCM samples than in peritumor samples. TIMM13 was strongly associated with sample type, subgroup, cancer stage, lymph node stage, and worse survival. Further, upregulation of TIMM13 was significantly associated with immunoregulators, and chemokines, as well as T cells, B cells, monocytes, neutrophils, macrophages, and T-cell regulators. An analysis of bioinformatic data uncovered that TIMM13 expression was strongly associated with PD1 (T-cell exhaustion marker). The nomogram showed good predictive performance based on calibration plot. TIMM13 was highly expressed in melanoma tissue samples than in normal samples.ConclusionIn brief, TIMM13 may be a prognostic biomarker for SKCM. It might modulate the tumor immune microenvironment and lead to a poorer prognosis. In addition, it is necessary to study the targeted therapy of TIMM13.

Identification of Timm13 protein translocase of the mitochondrial inner membrane as a potential mediator of liver fibrosis based on bioinformatics and experimental verification

“…Recently, Kim et al found that Timm13 showed reduced expression in human Alzheimer's disease brain tissues [45]. The latest research demonstrated that the expression of Timm13 in cutaneous melanoma (SKCM) tissues was higher than that in adjacent tissues, and Timm13 expression was closely related to programmed cell death protein 1 (PD1), suggesting that it might regulate the tumor immune microenvironment and affect prognosis [46]. Research on the relationship between translocase of the mitochondrial inner membrane and various diseases has gradually increased and several articles have reported on Timm13 in the past two years.…”

Analysis of the Prognostic and Immunological Role of HSPB1 in Pituitary Adenoma: A Potential Target for Therapy

“…The mRNA expression of HSPB1 in different cancer types was analysed in the TIMER database (4 January 2023) (http://cistrome.org/TIMER/) [12] and Xiantao online tools (4 January 2023) (https://www.xiantao.love) [13]. The protein expression of HSPB1 in different cancer types was analysed in the Human Protein Atlas database (5 January 2023) (https://www.proteinatlas.org/) [14].…”

“…The correlation between HSPB1 expression and survival in pan-cancers was analysed in the Xiantao online tools (6 January 2023) (https://www.xiantao.love) [13].…”