Timing of blastocyst development is the sole predictor of positive pregnancy outcome in advanced maternal age patients following single euploid blastocyst transfer

Abstract

estradiol levels at trigger (p-trend¼0.04) despite no differences in the total dose of gonadotropins. Specifically, women in the highest quartile of temperature (76.0-81.4 F) had an average estradiol level of 3761 pg/mL (95% CI 3403, 4119) compared to 3341 pg/mL (95% CI 3034, 3647) among women in the lowest quartile (38.6-49.6 F). There was no impact of temperature on oocyte counts. Lower temperatures and higher humidity prior to oocyte retrieval were associated with a slightly higher percentage of usable embryos after oocyte warming and fertilization (p-trend¼0.03 and 0.04). Greater mean precipitation prior to oocyte retrieval was associated with a slightly higher percentage of mature oocytes retrieved (p-trend¼0.06) but was not associated with any of the IVF outcomes.. CONCLUSIONS: While warmer temperatures prior to oocyte retrieval were associated with higher estradiol levels at trigger, the resulting oocytes resulted in a lower percentage of useable embryos once thawed and fertilized among recipients. Vitrified oocyte donation banks represent an excellent model to determine the impact of environmental exposure such as climate variables on IVF outcomes given that exposures experienced by the donor and recipient are uncorrelated in time and space.SUPPORT: Supported in part by R00ES026648 from the NIEHS.