Timesaving microwave assisted synthesis of insulin amyloid fibrils with enhanced nanofiber aspect ratio

Carvalho, Tiago; Pinto, Ricardo J.B.; Martins, Manuel A.; Silvestre, Armando J. D.; Freire, Carmen S.R.

doi:10.1016/j.ijbiomac.2016.07.008

Cited by 7 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The morphology and yield can be adjusted by varying the incubation time, temperature, pH, and ionic strength ( Moayedzadeh et al, 2015 ). Furthermore, microwave irradiation ( Carvalho et al, 2016 ) can shorten the time required to obtain the protein nanofibrils from days to a couple of hours. The use of alternative solvents with benign character as fibrillation agents (e.g., ionic liquids ( Silva et al, 2018a ) and deep eutectic solvents ( Silva et al, 2018c )) is also able to reduce the fibrillation time.…”

Section: Exploiting Protein Fibrils For the Production Of Novel Mater...mentioning

confidence: 99%

Cellulose and protein nanofibrils: Singular biobased nanostructures for the design of sustainable advanced materials

Silva

Silvestre

Vilela

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Polysaccharides and proteins are extensively used for the design of advanced sustainable materials. Owing to the high aspect ratio and specific surface area, ease of modification, high mechanical strength and thermal stability, renewability, and biodegradability, biopolymeric nanofibrils are gaining growing popularity amongst the catalog of nanostructures exploited in a panoply of fields. These include the nanocomposites, paper and packaging, environmental remediation, electronics, energy, and biomedical applications. In this review, recent trends on the use of cellulose and protein nanofibrils as versatile substrates for the design of high-performance nanomaterials are assessed. A concise description of the preparation methodologies and characteristics of cellulosic nanofibrils, namely nanofibrillated cellulose (NFC), bacterial nanocellulose (BNC), and protein nanofibrils is presented. Furthermore, the use of these nanofibrils in the production of sustainable materials, such as membranes, films, and patches, amongst others, as well as their major domains of application, are briefly described, with focus on the works carried out at the BioPol4Fun Research Group (Innovation in BioPolymer based Functional Materials and Bioactive Compounds) from the Portuguese associate laboratory CICECO–Aveiro Institute of Materials (University of Aveiro). The potential for partnership between both types of nanofibrils in advanced material development is also reviewed. Finally, the critical challenges and opportunities for these biobased nanostructures for the development of functional materials are addressed.

show abstract

Section: Exploiting Protein Fibrils For the Production Of Novel Mater...mentioning

confidence: 99%

Cellulose and protein nanofibrils: Singular biobased nanostructures for the design of sustainable advanced materials

Silva

Silvestre

Vilela

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Exploring underutilized biopolymeric nanostructures, such as protein amyloid NFs, might reveal a promising strategy to answer this problem . Protein NFs are nanostructures that are formed from the stacking of β-sheet-misfolded proteins or peptides, which can occur naturally or be produced in vitro. , These stacks result in highly organized quaternary structures stabilized by hydrogen bonds, inducing a rigid internal order to the fibrils . Typically, these structures present a morphology of unbranched filaments helically twisted along their axis .…”

Section: Introductionmentioning

confidence: 99%

Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium

Carvalho

Bártolo

Pedro

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Biopolymeric injectable hydrogels are promising biomaterials for myocardial regeneration applications. Besides being biocompatible, they adjust themselves, perfectly fitting the surrounding tissue. However, due to their nature, biopolymeric hydrogels usually lack desirable functionalities, such as antioxidant activity and electrical conductivity, and in some cases, mechanical performance. Protein nanofibrils (NFs), such as lysozyme nanofibrils (LNFs), are proteic nanostructures with excellent mechanical performance and antioxidant activity, which can work as nanotemplates to produce metallic nanoparticles. Here, gold nanoparticles (AuNPs) were synthesized in situ in the presence of LNFs, and the obtained hybrid AuNPs@LNFs were incorporated into gelatin-hyaluronic acid (HA) hydrogels for myocardial regeneration applications. The resulting nanocomposite hydrogels showed improved rheological properties, mechanical resilience, antioxidant activity, and electrical conductivity, especially for the hydrogels containing AuNPs@LNFs. The swelling and bioresorbability ratios of these hydrogels are favorably adjusted at lower pH levels, which correspond to the ones in inflamed tissues. These improvements were observed while maintaining important properties, namely, injectability, biocompatibility, and the ability to release a model drug. Additionally, the presence of AuNPs allowed the hydrogels to be monitorable through computer tomography. This work demonstrates that LNFs and AuNPs@LNFs are excellent functional nanostructures to formulate injectable biopolymeric nanocomposite hydrogels for myocardial regeneration applications.

show abstract

Self-assembly of N-terminal Alzheimer’s β-amyloid and its inhibition

Prasanna

Jing

2021

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Timesaving microwave assisted synthesis of insulin amyloid fibrils with enhanced nanofiber aspect ratio

Cited by 7 publications

References 30 publications

Cellulose and protein nanofibrils: Singular biobased nanostructures for the design of sustainable advanced materials

Cellulose and protein nanofibrils: Singular biobased nanostructures for the design of sustainable advanced materials

Injectable Nanocomposite Hydrogels of Gelatin-Hyaluronic Acid Reinforced with Hybrid Lysozyme Nanofibrils-Gold Nanoparticles for the Regeneration of Damaged Myocardium

Self-assembly of N-terminal Alzheimer’s β-amyloid and its inhibition

Contact Info

Product

Resources

About