Time-Varying Magnetic Fields: Effect on DNA Synthesis

Ar, Liboff; Williams, Taffy J.; Dm, Strong; Wistar, Richard

doi:10.1126/science.6695183

Cited by 341 publications

(110 citation statements)

References 13 publications

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“…Both the transient initial release of Ca 2+ from intracellular stores and the influx through specialized Ca 2+ channels operate the dephosphorylation of the cytoplasmic components (NFAT2 proteins) and give rise to their nuclear localization, which is followed by the activation of osteoclast-specific genes [6]. Pulsed electromagnetic fields (PEMFs) are useful therapy for patients with delayed fracture healing and nonunions [21] [22] [23] and affect different aspects of biomolecular synthesis in cells, including the kinetics of DNA, RNA, and protein production [24] [25]. In previous studies, research has focused of the effects of PEMF on osteoblasts and osteoclasts respectively, but the proliferation and activation of OC in OB-OC co-culture during PEMF stimulation has not been fully elucidated.…”

Section: Discussionmentioning

confidence: 99%

Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co-Cultured with Osteoblasts

Chen¹,

Huang²,

He³

et al. 2017

JBiSE

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The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) in rat osteoblast and osteoclast co-cultured model. Osteoblast-like cells were isolated from calvariae of Newborn Sprague Dawley rats (SD rats), while osteoclast-like cells were obtained from femora and tibiae of five weeks old SD rats. After 1 days of co-culture, the cells were exposed to premarin (E 2 ) and different frequencies of PEMFs (8 Hz and 16 Hz, respectively) for 3 days. The expression of RANK and NFATc1 mRNA was analysed with realtime quantitative polymerase chain reaction. The gene expression of RANK and NFATc1 in the E 2 , PEMF with 8 Hz and 16 Hz group was significantly lower than that in the control group respectively. The gene expression of NFATc1 in the PEMF with 8 Hz group was significantly lower than that in the control group and PEMF with 16 Hz group. The study indicates that PEMF with 8 Hz could regulate the gene expression of RANK and NFATc1 in co-cultured model.

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Section: Discussionmentioning

confidence: 99%

Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co-Cultured with Osteoblasts

Chen¹,

Huang²,

He³

et al. 2017

JBiSE

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“…A study found PEMF affect only the osteoclast phenotype after short exposure times [42]. It has been determined that electric stimulation can enhance osteoblast differentiation [43], increase osteoblast proliferation and consequently increase bone formation [44,45]. The mechanism or PEMF to inhibit bone loss is not well understood.…”

Section: Figurementioning

confidence: 99%

Study on Pulsed Electromagnetic Fields Regulate the mRNA Expression of V-ATPase, CAII, NFAT2 and RANK in an Ovariectomized Rat Model of Osteoporosis Mechanism Analysis

Huang¹,

Chen²,

He³

et al. 2017

J Bioengineer & Biomedical Sci

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We investigated the mRNA expression of V-ATPase CA II, NFAT2, and RANK as well as bone mineral density (BMD) in bilaterally ovariectomized (OVX) rats treated with pulsed electromagnetic fields (PEMF), to explore the mechanism by which PEMF alter bone at the molecular level in vivo. 24 Sprague-Dawley female rats were randomly divided into three different groups: SHAM, OVX, ovariectomy with PEMF stimulation (PEMF+OVX). The PEMF frequency was 3.8 mT, 8 Hz, for 40 min per day. Eight rats in each group were treated for 28 days. At the end of the intervention, the body weight and BMD were measured. The mRNA expression of V-ATPase, CA II, NFAT2 and RANK were analyzed by real-time fluorescent-nested quantitative polymerase chain reaction (RT-PCR). The V-ATPase, CAII, NFAT2 and RANK genes show strong expression in the OVX group as compared with the SHAM group (P<0.001, respectively). The mRNAs expression of V-ATPase, CAII, NFAT2 and RANK in PEMF treatment group were significantly lower than that in OVX group, respectively (p=0.001, p=0.000, p=0.013, P=0.04, respectively). The V-ATPase, CAII and RANK mRNA expression in the PEMF+OVX group were higher than SHAM group (p=0.04, p=0.001, p=0.000, respectively). However, the NFAT2 mRNA expression did not show a statistically significant difference between the SHAM and the PEMF+OVX group (P=0.08). This study indicated that exposure to PEMF restrained bone resorption in order to elevate BMD, and this outcome occurred in part through RANK-signaling by the CN/NFAT pathway to regulate bone metabolism. Alternatively, PEMF may promote the BMD through regulating the expression of NFAT2 genes. It may have certain significance in the treatment target of osteoporosis.

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“…As evidências biológicas disponíveis parecem indicar que sua ação se daria numa etapa mais tardia da carcinogênese, através de um efeito promotor ou co-promotor da doença 28,29,38,42,69,78 .…”

Section: Introductionunclassified

Mortalidade por câncer em trabalhadores de companhia geradora de eletricidade do Estado de São Paulo, Brasil

Mattos

Koifman

1996

Rev. Saúde Pública

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ResumoRealizou-se a análise exploratória de um conjunto de atestados de óbito de eletricitários do Estado de São Paulo (Brasil), através da Razão de Mortalidade Proporcional (PMR) e da Razão de Mortalidade Proporcional por Câncer (PCMR). Foi observado um discreto excesso de mortalidade por todas as localizações de câncer (PMR 1,11; IC 95% 0,91-1,35), estatisticamente não significativo. Encontrou-se elevação da PCMR, estatisticamente significativa, para câncer de laringe (PCMR 2,04; IC 95% 1,05-4,20). Um excesso de óbitos, embora sem significação estatística, foi observado para cavidade oral/faringe, próstata, bexiga, cérebro e doença de Hodgkin. A análise por categorias de exposição estimada a campos magnéticos mostrou PCMRs persistentemente elevadas para cancêr de bexiga (PCMR 4,17; IC 95% 1,35-9,72), cérebro (PCMR 3,77; IC 95% 1,02-9,65) e doença de Hodgkin (PCMR 5,55; IC 95% 1,14-16,21), no grupo com exposição provavelmente alta. Uma comparação com a mortalidade por câncer, de um grupo de trabalhadores petroquímicos, mostrou mortalidade elevada por neoplasma de laringe (PCMR 3,51; IC 95% 1,75-6,28) e bexiga (PCMR 7,53; IC 95% 3,02-15,51). Para tumores de cérebro, observou-se PCMR de 0,74 (IC 95% 0,61

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Time-Varying Magnetic Fields: Effect on DNA Synthesis

Cited by 341 publications

References 13 publications

Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co-Cultured with Osteoblasts

Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co-Cultured with Osteoblasts

Study on Pulsed Electromagnetic Fields Regulate the mRNA Expression of V-ATPase, CAII, NFAT2 and RANK in an Ovariectomized Rat Model of Osteoporosis Mechanism Analysis

Mortalidade por câncer em trabalhadores de companhia geradora de eletricidade do Estado de São Paulo, Brasil

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