Time trend of exposure to the phthalate plasticizer substitute DINCH in Germany from 1999 to 2017: Biomonitoring data on young adults from the Environmental Specimen Bank (ESB)

Kasper-Sonnenberg, Monika; Koch, Holger M.; Apel, Petra; Rüther, Maria; Pälmke, Claudia; Brüning, Thomas; Kolossa-Gehring, Marike

doi:10.1016/j.ijheh.2019.07.011

Cited by 66 publications

(32 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with these findings, we observed a negative time trend between 2014 and 2017 in maternal urinary concentration of several phthalate metabolites (DEHP, BBzP and DiBP), while maternal urinary concentrations of DINCH metabolites increased during this period. Positive time trends have been reported for DINCH metabolites in Sweden ( Gyllenhammar et al, 2017 ), Germany ( Kasper-Sonnenberg et al, 2019 ) and the USA ( Silva et al, 2013 ) and are consistent with the increase in use of this plasticizer reported in Europe ( European Chemicals Agency, 2013 ; HBM4EU, 2018 ). This, along with the higher urinary median concentrations observed at twelve months for DINCH metabolites in our cohort, highlights the need of detailed investigations to warrant its safety.…”

Section: Discussionsupporting

confidence: 80%

“…In addition, newer phthalates such as bis(2-propylheptyl) phthalate (DPHP) and phthalate's substitutes (1,2-Cyclohexane dicarboxylic acid diisononyl ester (DINCH)) have recently been marketed. DINCH, mainly used to produce plastics for toys, medical devices and food contact materials was introduced in the European market in 2002 ( Kasper-Sonnenberg et al, 2019 ). Positive temporal trends, suggesting an increase in exposure over time, have been reported for the DINCH metabolites in Sweden ( Gyllenhammar et al, 2017 ) and Germany ( Kasper-Sonnenberg et al, 2019 ), however, to date, no data regarding DINCH exposure in the French general population is available.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pre- and early post-natal exposure to phthalates and DINCH in a new type of mother-child cohort relying on within-subject pools of repeated urine samples

Philippat

Rolland

Lyon-Caen

et al. 2021

Environmental Pollution

View full text Add to dashboard Cite

For non-persistent chemicals such as phthalates, a single spot urine sample only reflects exposure in the past few hours. Collecting repeated urine samples for each participant over windows of sensitivity is expected to improve exposure characterization but has rarely been done. We aimed to rely on within-subject pools of repeated urine samples to assess phthalate exposure during pregnancy and infancy. Women of the French SEPAGES mother-child cohort were asked to collect three urine samples per day over seven consecutive days, twice during their pregnancy (approximatively second (T2) and third (T3) trimesters). For their infants they also collected one sample per day during a week at two (M2) and twelve months (M12). Samples were pooled (within-subject, within-period) prior to phthalate and DINCH metabolite concentrations assessment. Number of pooled samples assayed was 477, 456, 152 and 100 for T2, T3, M2 and M12, respectively. All metabolites were detected in more than 95% of the pooled samples except for the two DINCH metabolites (oh- and oxo-MINCH), MMCHP and oh-MPHP at M2 for which detection frequencies ranged between 64% and 88%. Maternal concentrations of MiBP, MBzP, DEHP metabolites and oxo-MiNP decreased between 2014 and 2017, whereas concentrations of oh-MiNP and the two DINCH metabolites increased (Mann-Kendall p-values < 0.05). While improved compared to studies that relied on spot samples, Intraclass Correlation Coefficients for the pregnancy were below 0.40 for most metabolites. Spearman correlation coefficients between pooled samples collected in infancy were lower than those observed during pregnancy, and were all below 0.30. Exposure to emerging phthalate substitutes such as DINCH and DPHP seems widespread among pregnant women and infants. Collecting repeated urine samples in pregnant women and infants is feasible. The relatively low correlation across trimesters and between maternal and infant samples highlights the need to collect biospecimens in the assumed sensitive time window.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Pre- and early post-natal exposure to phthalates and DINCH in a new type of mother-child cohort relying on within-subject pools of repeated urine samples

Philippat

Rolland

Lyon-Caen

et al. 2021

Environmental Pollution

View full text Add to dashboard Cite

show abstract

“…However, the concentrations of MINCH metabolites, OH-MINCH, cx-MINCH and oxo-MINCH, increased over the years and were detected to be 2.09, 0.86 and 1.81 μg/L, respectively in urine samples of the German Environmental Specimen Bank (Schütze et al, 2014). Moreover, several other studies have also detected DINCH metabolites in urine samples (Fromme et al, 2016;Kasper-Sonnenberg et al, 2019;Mínguez-Alarc on et al, 2016;Ramos et al, 2016;Schwedler et al, 2020;Urbancova et al, 2019). The tolerable daily intake is estimated to be 1 mg/kg bw/day based on the rat data (EFSA, 2006).…”

Section: Introductionmentioning

confidence: 94%

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) alters transcriptional profiles, lipid metabolism and behavior in zebrafish larvae

Saad

Bereketoğlu

Pradhan

2021

Heliyon

View full text Add to dashboard Cite

Plasticizers are commonly used in different consumer goods and personal care products to provide flexibility, durability and elasticity to polymers. Due to their reported toxicity, the use of several plasticizers, including phthalates has been regulated and/or banned from the market. Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) is an alternative plasticizer that was introduced to replace toxic plasticizers. Increasing global demand and lack of toxicity data and safety assessment of DINCH have raised the concern to human and animal health. Hence, in the present study, we investigated the adverse effects of DINCH (at concentrations ranging from 0.01 to 10 μM) in early developmental stages of zebrafish using different endpoints such as hatching rate, developmental abnormalities, lipid content, behavior analysis and gene expression. We found that DINCH caused hatching delay in a dose-dependent manner and altered the expression of genes involved in stress response. Lipid staining using Oil Red O stain showed a slight lipid accumulation around the yolk, brain, eye and neck with increasing concentration. Genes associated with lipid transport such as fatty acid synthesis, β-oxidation, elongation, lipid transport were significantly altered by DINCH. Genes involved in cholesterol biosynthesis and homeostasis were also affected by DINCH indicating possible developmental neurotoxicity. Behavioral analysis of larvae demonstrated a distinct locomotor activity upon exposure to DINCH. The present data shows that DINCH could induce physiological and metabolic toxicity to aquatic organisms. Hence, further analyses and environmental monitoring of DINCH should be conducted to determine its safety and toxicity levels.

show abstract

“…However, the concentrations of cyclohexane-1,2-dicarboxylic acid monoisononyl ester (MINCH) metabolites, OH-MINCH, cx-MINCH and oxo-MINCH, increased over the years and were detected to be 2.09, 0.86 and 1.81 μg/L, respectively in urine samples of the German Specimen Bank (Schütze et al, 2014). Moreover, several other studies have also detected DINCH metabolites in urine samples (Fromme et al, 2016;Kasper-Sonnenberg et al, 2019;Mínguez-Alarcón et al, 2016;Ramos et al, 2016;Schwedler et al, 2020;Urbancova et al, 2019). The tolerable daily intake is estimated to be 1 mg/kg bw/day based on the rat data (EFSA, 2006).…”

Section: Introductionmentioning

confidence: 98%

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) Alters Transcriptional Profiles, Lipid Metabolism and Behavior in Zebrafish Larvae

Saad

Bereketoğlu

Pradhan

2021

Preprint

View full text Add to dashboard Cite

Plasticizers are commonly used in different consumer goods and personal care products to provide flexibility, durability and elasticity to polymers. Due to their reported toxicity, the use of several plasticizers including phthalates has been regulated and/or banned from the market. Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) is an alternative plasticizer that was introduced to replace toxic plasticizers. Increasing global demand and lack of information regarding toxicity and safety assessment of DINCH have raised the concern to human and animal health. Hence, in the present study, we investigated the adverse effects of DINCH (at concentrations ranging from 0.01 to 10 μM) in early developmental stages of zebrafish using different endpoints such as hatching rate, developmental abnormalities, lipid metabolism, behavior analysis and gene expression. We found that DINCH caused hatching delay in a dose-dependent manner and altered the expression of genes involved in stress response. Lipid staining using Oil Red O stain showed a slight lipid accumulation around the yolk, brain, eye and neck with increasing concentration. Genes associated with lipid metabolism such as fatty acid synthesis, β-oxidation, elongation, lipid transport were significantly altered by DINCH. Behavioral analysis of larvae demonstrated a distinct locomotor activity including distance and acceleration upon exposure to DINCH both in light and dark. Genes involved in cholesterol biosynthesis and homeostasis were also affected by DINCH indicating possible developmental neurotoxicity. The present data shows that DINCH could induce physiological and metabolic toxicity to aquatic organisms. Hence, further analyses and environmental monitoring on DINCH should be conducted to determine its safety and toxicity levels.

show abstract

Time trend of exposure to the phthalate plasticizer substitute DINCH in Germany from 1999 to 2017: Biomonitoring data on young adults from the Environmental Specimen Bank (ESB)

Cited by 66 publications

References 42 publications

Pre- and early post-natal exposure to phthalates and DINCH in a new type of mother-child cohort relying on within-subject pools of repeated urine samples

Pre- and early post-natal exposure to phthalates and DINCH in a new type of mother-child cohort relying on within-subject pools of repeated urine samples

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) alters transcriptional profiles, lipid metabolism and behavior in zebrafish larvae

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) Alters Transcriptional Profiles, Lipid Metabolism and Behavior in Zebrafish Larvae

Contact Info

Product

Resources

About