Time‐stretch Chromatic Confocal Microscopy for Multi‐Depth Imaging

Yang, Xuefang; Zhang, Han; Liu, Zhexi; Fan, Yubo; Yue, Shuhua; Ma, Dinglong; Chen, Xun; Wang, Pu

doi:10.1002/lpor.202300387

Cited by 5 publications

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The development and progress of confocal laser scanning microscopy (CLSM) have promoted the progress of biology, basic medicine and other disciplines, whose high-quality images provide a basis and guidance for understanding, analyzing, and processing samples [1][2][3][4][5]. CLSM realizes the suppression of spurious signals outside the focal plane by setting a conjugate pinhole in the point scanning imaging system, which not only improves the lateral resolution, but also has the ability to resolve the sample axially, providing three-dimensional (3D) imaging ability [6][7]. Because it can clearly image thin samples such as cells with high resolution, it plays an irreplaceable role in cell research, oncology, hematology, neuroscience, and other fields.…”

Section: Introductionmentioning

confidence: 99%

Ultra-low-cost and high-fidelity NIR-II confocal laser scanning microscope with Bessel beam excitation and SiPM detection

Chen,

Wanng,

Yan

et al. 2024

Preprint

View full text Add to dashboard Cite

The NIR-II based CLSM has problems such as expensive detector and reduced image resolution. Here, by simultaneously using a low-cost silicon photomultiplier (SiPM) as a detector and a Bessel beam as an excitation, we developed an ultra-low-cost and high-fidelity NIR-II confocal laser scanning microscope. The introduction of Bessel beam compensates to some extent for the weakening of spatial resolution caused by the increase in the wavelength of light in the NIR region. The use of SiPM reduces the cost of NIR-II fluorescence detection module in CLSM, while enabling the detection of ultra-broadband fluorescence signals spanning visible to NIR-II regions.

show abstract

Section: Introductionmentioning

confidence: 99%

Ultra-low-cost and high-fidelity NIR-II confocal laser scanning microscope with Bessel beam excitation and SiPM detection

Chen,

Wanng,

Yan

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Ultra-low-cost and high-fidelity NIR-II confocal laser scanning microscope with Bessel beam excitation and SiPM detection

Wang,

Yan,

Wang

et al. 2024

Biomed. Opt. Express

View full text Add to dashboard Cite

Confocal laser scanning microscopy (CLSM) is one of the most important imaging tools in the biomedical field, and near-infrared-II (NIR-II, 900-1700nm) fluorescence imaging technology has also made fruitful research progress in deep imaging in recent years. The NIR-II based CLSM has problems such as an expensive detector and reduced image resolution caused by long wavelength excitation. Here, by simultaneously using a low-cost silicon photomultiplier (SiPM) as a detector and a Bessel beam as an excitation, we developed an ultra-low-cost and high-fidelity NIR-II confocal laser scanning microscope. The use of SiPM reduces the cost of the NIR-II fluorescence detection module in CLSM, while enabling the detection of ultra-broadband fluorescence signals spanning visible to NIR-II regions. The introduction of the Bessel beam compensates to some extent for the weakening of spatial resolution caused by the increase in the wavelength of light in the NIR region. Experimental results show that the use of the Bessel beam can improve the resolution by 12% when observing thin samples. With the increase of sample thickness, the imaging resolution of the Bessel beam at NIR-II wavelengths is better than that of the Gaussian beam at NIR-I wavelengths at the penetrable depth of the NIR-I light. At deeper depths, the imaging resolution and imaging depth of Bessel beam CLSM is superior to Gaussian beam CLSM at the same excitation power.

show abstract

Graded arc beam in light needle microscopy for axially resolved, rapid volumetric imaging without nonlinear processes

Kume,

Kozawa,

Kawakami

et al. 2024

Opt. Express

View full text Add to dashboard Cite

High-speed three-dimensional (3D) imaging is essential for revealing the structure and functions of biological specimens. Confocal laser scanning microscopy has been widely employed for this purpose. However, it requires a time-consuming image-stacking procedure. As a solution, we previously developed light needle microscopy using a Bessel beam with a wavefront-engineered approach [Biomed. Opt. Express 13, 1702 (2022)10.1364/BOE.449329]. However, this method applies only to multiphoton excitation microscopy because of the requirement to reduce the sidelobes of the Bessel beam. Here, we introduce a beam that produces a needle spot while eluding the intractable artifacts due to the sidelobes. This beam can be adopted even in one-photon excitation fluorescence 3D imaging. The proposed method can achieve real-time, rapid 3D observation of 200-nm particles in water at a rate of over 50 volumes per second. In addition, fine structures, such as the spines of neurons in fixed mouse brain tissue, can be visualized in 3D from a single raster scan of the needle spot. The proposed method can be applied to various modalities in biological imaging, enabling rapid 3D image acquisition.

show abstract

Time‐stretch Chromatic Confocal Microscopy for Multi‐Depth Imaging

Cited by 5 publications

References 43 publications

Ultra-low-cost and high-fidelity NIR-II confocal laser scanning microscope with Bessel beam excitation and SiPM detection

Ultra-low-cost and high-fidelity NIR-II confocal laser scanning microscope with Bessel beam excitation and SiPM detection

Ultra-low-cost and high-fidelity NIR-II confocal laser scanning microscope with Bessel beam excitation and SiPM detection

Graded arc beam in light needle microscopy for axially resolved, rapid volumetric imaging without nonlinear processes

Contact Info

Product

Resources

About