Time-series monitoring of transgenic maize seedlings phenotyping exhibiting glyphosate tolerance

Tao, Mingzhu; Feng, Xuping; He, Yong; Zhang, Jinnuo; Bai, Xiulin; Yang, Guofeng; Wei, Yuzhen

doi:10.1101/2022.03.21.485126

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…The increase in ABS/RC may be due to a reduced ratio of active RCs and an increase in absorbed light energy, which may be related to the deactivation of RCs or an increase in antenna size. Previous studies ( Tao et al., 2022 ) showed that in general, RCs inactivation of plants is related to photoinhibition, that is, when the amount of light absorbed by plants exceeds the amount of light that can be utilized by their own photosystem, the photosynthetic function of plants decreases. Derks et al.…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic and physiological responses of different peony cultivars to high temperature

Wen

Hong²,

Chen³

et al. 2022

Front. Plant Sci.

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In order to investigate the causes of the differences in heat tolerance (‘Lu He Hong’ and ‘Zhi Hong’), we studied the physiological changes, photosynthetic properties and regulatory mechanism of the two peony cultivars at high temperature. The results showed that the physiological changed of different peony cultivars varied significantly under high temperature stress. With the extension of high temperature stress time, MDA content of 'Lu He Hong' increased,while 'Zhi Hong' rised first and then decreased, SOD activity of 'Lu He Hong' rised first and then decreased, that of 'Zhi Hong' kept rising, POD activity of 'Lu He Hong' kept decreasing, while 'Zhi Hong' rised. The photosynthetic instrument records the change of peony photosynthesis parameters at high temperature; the chlorophyll A (Chla) fluorescence transient is recorded using the plant efficiency analyzer (PEA), analyzed according to the JIP test (O-J-I-P fluorescence transient analysis), and several parameters were derived to explain the photosynthetic efficiency difference between different peony cultivars. The tested cultivars responded differently to the survey conditions, and the PCA analysis showed that the ‘Zhi Hong’ was more well tolerated and showed better thermal stability of the PSII. The reduced efficiency of the ‘Lu He Hong’ PSII antenna leads to higher heat dissipation values to increase the light energy absorbed by unit reaction center (ABS/RC), the energy captured by unit reaction center (TR0/RC), and the energy dissipated by unit reaction center (DI0/RC), which significantly leads to its lower total photosynthetic performance (PItotal). The light capture complex of the variety ‘Zhi Hong’ has high connectivity with its reaction center, less damage to OEC activity, and better stability of the PSII system. The results show that ‘Zhi Hong’ improves heat resistance by stabilizing the cell membrane, a strong antioxidant system, as well as a more stable photosynthetic system. The results of this study provide a theoretical basis for the screening of heat-resistant peonies suitable for cultivation in Jiangnan area and for the selection and breeding of heat-resistant cultivars.

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Section: Discussionmentioning

confidence: 99%

Photosynthetic and physiological responses of different peony cultivars to high temperature

Wen

Hong²,

Chen³

et al. 2022

Front. Plant Sci.

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Lack of Significant Effects of Glyphosate on Glyphosate-Resistant Maize in Different Field Locations

et al. 2023

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Glyphosate-resistant (GR) maize is dominant in countries where it is grown. Significant, adverse effects of glyphosate application to GR maize have been reported, but few data from robust studies exist to determine if such effects are common. In this study, the effects of recommended application rates (single and sequential applications) were used on GR maize grown at two locations for one season and for two seasons in a third location. No significant effects of glyphosate on mineral content (N, P, K, Ca, Mg, S, Cu, Fe, Mn, and Zn) in leaves or grain, plant height, stem diameter, ear parameters, or yield were found at any location or in any growing season. Likewise, harvested grain quality, as determined by percent starch, protein, and total lipids, was unaffected by glyphosate treatment at any location. Neither glyphosate nor aminomethylphosphonic acid, the primary degradation product of glyphosate, were found in grain from any treatment at any location, except for 20 ng g−1 of glyphosate found in grain from one season at one location. These results support the view that recommended applications of glyphosate have no significant effects on growth, grain composition, mineral content, grain quality, nor yield of GR maize.

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Study on Heat Resistance of Peony Using Photosynthetic Indexes and Rapid Fluorescence Kinetics

Wen

Hong

et al. 2023

Horticulturae

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To investigate the effects of high-temperature stress on the chlorophyll fluorescence induction kinetics of peony and to determine indicators for the rapid screening of varieties responding to high temperatures, three four-year-old peony variety, ‘Fengdanbai’, ‘Huhong’, and ‘Yinhongqiaodui’, were selected as materials. The photosynthetic curves (Pn-PAR) and fast chlorophyll fluorescence curves (OJIP curves) of peony leaves were measured at different times under high-temperature stress conditions (40 °C), the changes in the photosynthetic characteristics of different peony varieties under high-temperature stress were analyzed, and the heat tolerance of peony was evaluated. The results showed that ‘Huhong’ grew well within 16 days, while all of the other varieties showed obvious wilting at 6–9 days. High temperatures damaged the structure and function of the photosystem of peony leaves, indicating that the maximum net photosynthetic rate (Pnmax), apparent quantum efficiency (AQE), maximum photochemical efficiency (Fv/Fm), and photosynthetic performance index (PIABS) all tended to decrease under high-temperature stress, while the rate of closing the PS II reaction center (Mo) and the absorption per reaction center (ABS/RC), the capture (TRo/RC), and the dissipation (Dio/RC) of light energy per reaction center showed an overall increasing trend. The ability to cope with high-temperature stress differed among varieties, and the heat tolerance was determined to be in the descending order of ‘Fengdanbai’ < ‘Yinhongqiaodui’ < ‘Huhong’. The correlation analysis among the parameters and the analysis of the morphological change patterns in peony leaves concluded that PIABS, Dio/RC, and Mo could be used as indicators of peony tolerance to high-temperature stress. The results of the study can provide a basis for the screening of heat-tolerant peony species and peony heat defense in the Jiangnan area.

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Time-series monitoring of transgenic maize seedlings phenotyping exhibiting glyphosate tolerance

Cited by 3 publications

References 41 publications

Photosynthetic and physiological responses of different peony cultivars to high temperature

Photosynthetic and physiological responses of different peony cultivars to high temperature

Lack of Significant Effects of Glyphosate on Glyphosate-Resistant Maize in Different Field Locations

Study on Heat Resistance of Peony Using Photosynthetic Indexes and Rapid Fluorescence Kinetics

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