Time-sequential modulation in expression of growth factors from platelet-rich plasma (PRP) on the chondrocyte cultures

Abstract

Platelets are involved in hemostasis, wound healing, and tumor growth. Autologous blood products are commonly used to facilitate healing in a variety of clinical surgery applications. Recently, it was shown that platelet-rich plasma (PRP) has more specific growth factors that participate in the healing process. This study investigated the expression of PRP growth factors and evaluated their potential role in the cartilage regeneration using primary isolated chondrocytes. PRP obtained from New Zealand White rabbit by low speed centrifugation. Extracted PRPs contained 6-10 × 10(6) platelet/μl and concentration of platelets was slightly variable. Primary isolated chondrocytes from the same rabbits were cultured and treated with 0.1-20% PRP. The cells were collected and examined by reverse transcription-polymerase chain reaction and cytochemical staining. The expression of sex determining region Y-box 9, transforming growth factor-beta, vascular endothelial growth factor, and chondromdulin-I was increased in chondrocyte cultures with 10% PRP by time-dependent manner. To maintain the integrity of the cartilage, the proteoglycan contents were also up-regulated from the mRNA of aggrecan and positive Safranin-O staining in PRP concentration- and time-dependent manner. PRP provides crucial growth factors related to chondrocyte proliferation and differentiation through time-sequential modulation. Controlled in vivo trials for cartilage regeneration are needed.

Human platelet releasates combined with polyglycolic acid scaffold promote chondrocyte differentiation and phenotypic maintenance

“…PRPr can be an economic and valuable source for autologous and highly concentrated growth and differentiation factors (Wu et al 2007;Sawamura et al 2009), whose application in clinical practice (Borzini and Mazzucco, 2005;Smith and Roukis, 2009;Lopez-Vidriero et al 2010;Andia et al 2011) as well as in cartilage tissue engineering (Anitua et al 2005;Gaissmaier et al 2005;Kanno et al 2005;Mishra et al 2009;Wu et al 2011;Park et al 2012) has been widely documented. In particular, in a previous study we have reported the efficacy of PRP releasates obtained by means of an optimized and standardized protocol to maintain chondrocyte-like phenotype in monolayer cultures of human articular chondrocytes (Spreafico et al 2009).…”

The Effect of Platelet-Rich Plasma on Autologous Osteochondral Transplantation

“…On gross assessment, the PRP-treated graft borders were less demarcated compared with the control grafts, and there was a significant improvement in scoring of the integration at the cartilage interface on histological assessment. Chondrocyte viability and proliferation are increased in the presence of PRP 50,52,53 . Additionally, chondrocytes cultured with PRP may increase their proteoglycan deposition and type-II collagen content 22,24,53 , potentially increasing the quality and quantity of repair tissue at the chondral interface.…”

The role of platelet rich plasma injection in the management of early osteoarthritis of the knee

“…PRP brings about its effect in osteoarthritis by stimulating cartilage matrix metabolism. [14][15][16][17] Since it is autologous, there is no risk of allergic reactions or disease transmission. There are no side effects.…”