Time-resolved W-band (95 GHz) EPR spectroscopy of Zn-substituted reaction centers of Rhodobacter sphaeroides R-26

Prisner, Thomas F.; Est, Arthur van der; Bittl, Robert; Lubitz, Wolfgang; Stehlik, D.; Möbius, K.

doi:10.1016/0301-0104(95)00016-h

Cited by 112 publications

(157 citation statements)

References 24 publications

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“…This is in agreement with the experimental observations of Prisner et al (100). In their W-band time-resolved EPR study, pulsed field sweep echo spectra and transient direct detection spectra of the radical pair Ps+sQ~ have been compared.…”

Section: Secondary Radical Pairsupporting

confidence: 89%

“…The simulations obtained for the P+ (QXFe) spectra for quinone-replaced RC, even under inclusion of possible singlet-triplet mixing in the primary radical pair, are much worse than what is possible for the case of Zn-substituted RC with the native QA (see e.g. Prisner et al (100) and van den Brink et al (366). A likely reason for part of the discrepancies between experiment and simulation for P+ (QiFe) spectra is the neglect of spin relaxation in the model used.…”

Section: Secondary Radical Pairmentioning

confidence: 97%

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Radicals and Radical Pairs in Photosynthesis

Angerhofer

Bittl

1996

Photochem & Photobiology

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Section: Secondary Radical Pairsupporting

confidence: 89%

Section: Secondary Radical Pairmentioning

confidence: 97%

Radicals and Radical Pairs in Photosynthesis

Angerhofer

Bittl

1996

Photochem & Photobiology

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“…5). A quantitative analysis yields an angle of 62" between the quinone x-axis (along the C=O bonds) and the PQ distance vector zd (dipolar axis) for bRC [22], in accordance with the X-ray structure. Whereas, for PS I [27] the corresponding angle is close to 0".…”

Section: The P"q-' States In Different Rc-types (Brc and Ps I)supporting

confidence: 64%

“…However, this requires a knowledge of the orientation of the g-tensor axes of P&, relative to the molecular axes which has not been determined. Thus, we can only make an estimate based on general principles and using the axis orientations for P&6; in bRC's [22]. To a rough approximation, the x-axis is perpendicular to the plane of the page, the y-axis is parallel to the pseudo C2 symmetry axis, and the z-axis is perpendicular to the porphyrin planes.…”

Section: Structural Predictionsmentioning

confidence: 99%

Transient magnetic resonance. Transient spin states in the primary processes of photosynthesis

Stehli

Est

Kamlowski

1996

Ber Bunsenges Phys Chem

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The role of the first stabilized charge separated state, P+'Q-: generated in the course of the primary processes in photosynthesis is discussed. In particular, the adaptation of the properties of Q-' to the functional requirements in different types of photosynthetic reaction centers is considered. Time resolved EPR is shown to be very well-suited for the characterization of P+'Q-' and that important structural and functional properties of the radical pair can be obtained from such data. The most recent spectroscopic information is presented and evaluated with the aim of correlating differences in the observed characteristics of the quinone acceptor with differences in the various types of photosynthetic reaction centers. It is suggested that the nature of the acceptors following the primary quinone (either an exchangeable quinone or a series of iron-sulfur centers) has a marked influence on the nature of the quinone binding.

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“…5d). It is also possible to detect the transient magnetization using ESE detected EPR, sometimes even with higher sensitivity [24]. The spectra of the dyad system in Fig.…”

Section: Electron Transfer and Spin Dynamics Studied By Treprmentioning

confidence: 99%

Pulsed-High Field/High-Frequency EPR Spectroscopy

Fuhs

Möbius

2002

High Magnetic Fields

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Abstract. Pulsed high-field/high-frequency electron paramagnetic resonance (EPR) spectroscopy is used to disentangle many kinds of different effects often obscured in continuous wave (cw) EPR spectra at lower magnetic fields/microwave frequencies. While the high magnetic field increases the resolution of G tensors and of nuclear Larmor frequencies, the high frequencies allow for higher time resolution for molecular dynamics as well as for transient paramagnetic intermediates studied with time-resolved EPR. Pulsed EPR methods are used for example for relaxation-time studies, and pulsed Electron Nuclear DOuble Resonance (ENDOR) is used to resolve unresolved hyperfine structure hidden in inhomogeneous linewidths. In the present article we introduce the basic concepts and selected applications to structure and mobility studies on electron transfer systems, reaction centers of photosynthesis as well as biomimetic models. The article concludes with an introduction to stochastic EPR which makes use of another concept for investigating resonance systems in order to increase the excitation bandwidth of pulsed EPR. The limited excitation bandwidth of pulses at high frequency is one of the main limitations which, so far, made Fourier transform methods hardly feasible.

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Time-resolved W-band (95 GHz) EPR spectroscopy of Zn-substituted reaction centers of Rhodobacter sphaeroides R-26

Cited by 112 publications

References 24 publications

Radicals and Radical Pairs in Photosynthesis

Radicals and Radical Pairs in Photosynthesis

Transient magnetic resonance. Transient spin states in the primary processes of photosynthesis

Pulsed-High Field/High-Frequency EPR Spectroscopy

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