Time-resolved small-angle neutron scattering (TR-SANS) for structural biology of dynamic systems: Principles, recent developments, and practical guidelines

Martel, Anne; Gabel, Frank

doi:10.1016/bs.mie.2022.08.010

Cited by 3 publications

(3 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The latest advances in TR-SANS experiments permit the examination of the development of biological structures at a length scale ranging from nanometers to micrometers and over a timeframe that spans from seconds to days [29]. The duration of each time frame is dependent on various factors, such as the incoming neutron flux, collimation length, sample volume, sample concentration and contrast, incoherent background, and detector efficiency.…”

Section: Time-resolved Sans/saxs (Tr-sans/saxs)mentioning

confidence: 99%

“…This technique is particularly well-suited for analyzing these processes due to the substantial scattering signal generated by the voluminous and extended structures involved and the considerable changes in aggregation numbers that occur. Notably, TR-SANS can detect observable changes in parameters such as I(0) and Rg during these processes, adding to its effectiveness in this field of study [29]. TR-SANS was demonstrated to be a valuable tool in studying the kinetics of protein aggregation and fibrillation.…”

Section: Time-resolved Sans/saxs (Tr-sans/saxs)mentioning

confidence: 99%

“…SANS and SAXS have been previously utilized across diverse research domains such as the examination of food colloids [25], the advancement of mRNA vaccines [26], and in photosynthesis research (see [27,28] for reviews). The recent advancement of time-resolved SANS (TR-SANS) enables exploring dynamic processes within structural biology [29].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recent Progress in Solution Structure Studies of Photosynthetic Proteins Using Small-Angle Scattering Methods

Golub,

Pieper

2023

Molecules

View full text Add to dashboard Cite

Utilized for gaining structural insights, small-angle neutron and X-ray scattering techniques (SANS and SAXS, respectively) enable an examination of biomolecules, including photosynthetic pigment-protein complexes, in solution at physiological temperatures. These methods can be seen as instrumental bridges between the high-resolution structural information achieved by crystallography or cryo-electron microscopy and functional explorations conducted in a solution state. The review starts with a comprehensive overview about the fundamental principles and applications of SANS and SAXS, with a particular focus on the recent advancements permitting to enhance the efficiency of these techniques in photosynthesis research. Among the recent developments discussed are: (i) the advent of novel modeling tools whereby a direct connection between SANS and SAXS data and high-resolution structures is created; (ii) the employment of selective deuteration, which is utilized to enhance spatial selectivity and contrast matching; (iii) the potential symbioses with molecular dynamics simulations; and (iv) the amalgamations with functional studies that are conducted to unearth structure-function relationships. Finally, reference is made to time-resolved SANS/SAXS experiments, which enable the monitoring of large-scale structural transformations of proteins in a real-time framework.

show abstract

Section: Time-resolved Sans/saxs (Tr-sans/saxs)mentioning

confidence: 99%

Section: Time-resolved Sans/saxs (Tr-sans/saxs)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Recent Progress in Solution Structure Studies of Photosynthetic Proteins Using Small-Angle Scattering Methods

Golub,

Pieper

2023

Molecules

View full text Add to dashboard Cite

show abstract

Advances, Applications, and Perspectives in Small‐Angle X‐ray Scattering of RNA

Tants

Schlundt

2023

ChemBioChem

View full text Add to dashboard Cite

RNAs exhibit a plethora of functions far beyond transmitting genetic information. Often, RNA functions are entailed in their structure, be it as a regulatory switch, protein binding site, or providing catalytic activity. Structural information is a prerequisite for a full understanding of RNA‐regulatory mechanisms. Owing to the inherent dynamics, size, and instability of RNA, its structure determination remains challenging. Methods such as NMR spectroscopy, X‐ray crystallography, and cryo‐electron microscopy can provide high‐resolution structures; however, their limitations make structure determination, even for small RNAs, cumbersome, if at all possible. Although at a low resolution, small‐angle X‐ray scattering (SAXS) has proven valuable in advancing structure determination of RNAs as a complementary method, which is also applicable to large‐sized RNAs. Here, we review the technological and methodological advancements of RNA SAXS. We provide examples of the powerful inclusion of SAXS in structural biology and discuss possible future applications to large RNAs.

show abstract

Advances in sample environments for neutron scattering for colloid and interface science

Le Brun,

Gilbert

2024

Advances in Colloid and Interface Science

View full text Add to dashboard Cite

Time-resolved small-angle neutron scattering (TR-SANS) for structural biology of dynamic systems: Principles, recent developments, and practical guidelines

Cited by 3 publications

References 94 publications

Recent Progress in Solution Structure Studies of Photosynthetic Proteins Using Small-Angle Scattering Methods

Recent Progress in Solution Structure Studies of Photosynthetic Proteins Using Small-Angle Scattering Methods

Advances, Applications, and Perspectives in Small‐Angle X‐ray Scattering of RNA

Advances in sample environments for neutron scattering for colloid and interface science

Contact Info

Product

Resources

About