2000
DOI: 10.1021/bi0011779
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Time-Resolved Monitoring of Flash-Induced Changes of Fluorescence Quantum Yield and Decay of Delayed Light Emission in Oxygen-Evolving Photosynthetic Organisms

Abstract: The present contribution describes a new experimental setup that permits time-resolved monitoring of the rise kinetics of the relative fluorescence yield, Phi(rel)(t), and simultaneously of the decay of delayed light emission, L(t), induced by strong actinic laser flashes. The results obtained by excitation of dark-adapted samples with a train of eight flashes reveal (a) in suspensions of spinach thylakoids, Phi(rel)(t) exhibits a typical period four oscillation that is characteristic for a dependence on the r… Show more

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Cited by 30 publications
(41 citation statements)
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References 34 publications
(92 reference statements)
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“…for k L ) k s 1 : Equation 3c signifies that the F(t) rise is independent of intensity at high excitation rate k L and illustrates, in agreement with the F(t) response in a ns laser flash (Steffen 2003), its sole dependence on the rate constant (k s 1 ) of donor side quenching (DSQ) release under these conditions. The laser-induced excitation data (Steffen ks 1 ÀkL e ÀkLt þ kL ks 1 ÀkL e Àks 1 t (Eq.…”
Section: Theorysupporting
confidence: 64%
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“…for k L ) k s 1 : Equation 3c signifies that the F(t) rise is independent of intensity at high excitation rate k L and illustrates, in agreement with the F(t) response in a ns laser flash (Steffen 2003), its sole dependence on the rate constant (k s 1 ) of donor side quenching (DSQ) release under these conditions. The laser-induced excitation data (Steffen ks 1 ÀkL e ÀkLt þ kL ks 1 ÀkL e Àks 1 t (Eq.…”
Section: Theorysupporting
confidence: 64%
“…5 and 6), a sigmoidal rise of the MTF-induced fluorescence response curve. The sigmoidicity arises from the fact that the variable fluorescence Fv is controlled by the rate constant of light excitation k L and of release of donor side quenching k s 1 : Characteristics of donor side quenching can be read from the rise and decay kinetics of quenching release in ultra short single turnover excitations (Butler 1972;Mauzerall 1972;Steffen 2003;Steffen et al 2005;Belyaeva et al 2006). The 'degree' of sigmoidicity of F(t) is determined by the ratio between k L and k s 1 (Figs.…”
Section: Discussionmentioning
confidence: 99%
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“…F m is associated with full reduction of Q A and with the trappingincompetent closed RC. Other electron transport intermediates at the acceptor and donor sides of PSII have been proposed as additional functional quenchers like Phe (Phe) (Klimov et al 1977;Vredenberg 2000), reduced forms of the secondary quinone acceptor Q B - (Samson et al 1999;Yaakoubd et al 2002, Schreiber 2002 and Q B 2- (Zhu et al 2005), plastoquinone (PQ) (Vernotte et al 1979), oxidized primary (P 680 + ) (Butler 1972), and secondary donor (Y z + ) (Vredenberg et al 2001) or side products like triplet carotenoids (car T ) (Steffen et al 2001). Their quenching has been discussed in relation to the seeming paradox that the release of fluorescence quenching F v in a single turnover flash (STF) in which all Q A is reduced, is substantially below that measured in multi-turnover light pulses (MTF) (Kramer et al 1995;Samson and Bruce 1996;Kolber et al 1998;Vasilev and Bruce 1998;Samson et al 1999;Koblizek et al 2001;Vredenberg et al 2005).…”
Section: Introductionmentioning
confidence: 99%