Time-resolved immunofluorometric assay of 34-kDa somatomedin-binding protein.

Koistinen, R; Stenman, U H; Alfthan, Henrik; Seppälä, Matti

doi:10.1093/clinchem/33.7.1126

Cited by 39 publications

(3 citation statements)

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“…Insulin was measured by radioimmunoassay using a polyclonal antiserum (MF/GP/9) from Guildhay Ltd, Guildford, Surrey and IGF-I by RIA after acid-ethanol extraction of the serum using reagents from Amersham International, Amersham, Bucks (Daughaday et al, 1980). The 34kDa IGF-BP (IGF-BP-I, PP 12) was measured by an immunofluorometric assay as described by Koistinen et al (1987).…”

Section: Methodsmentioning

confidence: 99%

Diet‐induced Changes in Sex Hormone Binding Globulin and Free Testosterone in Women With Normal or Polycystic Ovaries: Correlation With Serum Insulin and Insulin‐like Growth Factor‐i

Kiddy

Hamilton‐Fairley

Seppälä

et al. 1989

Clinical Endocrinology

172

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The purpose of this study was to investigate the effect of calorie restriction on serum concentrations of sex hormone binding globulin (SHBG) in women with normal or polycystic ovaries (PCO) and to examine the possible role of insulin and insulin-like growth factor-I (IGF-I) in mediating changes in SHBG levels. Six normal subjects with mean (SD) body mass index (BMI) 25.5 (2.2) and five subjects with PCO (BMI 36.1 (3.7)) were studied before and after 2 or (PCO only) 4 weeks of a very low calorie diet (330 kcal/day; Cambridge Diet). In both normal women and patients with PCO there was a twofold increase in SHBG concentrations after 2 weeks and this was sustained in the PCO subjects for a further 2 weeks. The rise in SHBG was accompanied by a fall in free testosterone concentrations. There were parallel changes in serum insulin and IGF-I concentrations which decreased during the diet and there were significant negative correlations of SHBG with insulin in both normal subjects (r = -0.62) and women with PCO (r = -0.60). In addition, serum concentrations of an insulin-dependent small molecular weight (34 kDa) binding protein for IGF-I (IGF-BPI) increased significantly during dieting in both groups and were negatively correlated with serum insulin (controls, r = -0.56; PCO, r = -0.68) and positively correlated with serum SHBG levels (controls, r = 0.69; PCO, r = 0.63). In summary, these data indicate that in both normal subjects and those subjects with PCO, calorie restriction results in a highly significant increase in SHBG concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Methodsmentioning

confidence: 99%

Diet‐induced Changes in Sex Hormone Binding Globulin and Free Testosterone in Women With Normal or Polycystic Ovaries: Correlation With Serum Insulin and Insulin‐like Growth Factor‐i

Kiddy

Hamilton‐Fairley

Seppälä

et al. 1989

Clinical Endocrinology

172

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“…Blood samples were obtained for analysis of the serum concentrations of IGFBP‐1 and PP14 by immunofluorometric assays 4,5 with an inter‐assay coefficient of variation of less than 10%. The sensitivity of the PP14 assay, as used in the present study, was 3 μg/1.…”

Section: Methodsmentioning

confidence: 99%

Serum levels of endometrial proteins during transcervical resection of the endometrium

Hahn¹,

Olsson²,

Englund³

et al. 1996

BJOG

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Objective To study the potential for dissemination of endometrial tissue substances during transcervical resection of the endometrium (TCRE). Design Prospective study. Setting One university and two county hospitals. Participants Forty‐eight women with dysfunctional bleeding. Interventions The serum levels of two endometrial proteins, insulin‐like growth factor binding protein‐1 (IGFBP‐1) and placental protein 14 (PP14), were measured before and every 10 min during the operations. Blood loss was also measured by a photometer together with absorption of the irrigating fluid containing glycine 1.5% and ethanol 1% by expired‐breath tests, and serum sodium and volumetric fluid balances. Main outcome measures Linear correlations between changes in IGFBP‐1 and PP14 during TCRE and operating parameters such as operating time, blood loss and fluid absorption. Results The baseline levels of IGFBP‐1 were normal but PP14 could only be detected in one third of the patients, which was due, in part, to pre‐operative treatment with danazol. The highest levels of IGFBP‐1 and PP14 during surgery correlated positively with the baseline concentrations. Fluid absorption (median 405 ml, range 0–2177) was the only surgical factor associated with increasing serum levels of endometrial proteins. Conclusion Absorption of the solution used to irrigate the uterus is associated with dissemination of endometrial products in the bloodstream during TCRE.

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“…The IGFBP-1 concentration was measured by a specific monoclonal time-resolved immunofluorometric assay performed essentially as described earlier (Koistinen et al, 1987;Angervo et a/., 1992). The sensitivity of the assay was 0.1 pg/l.…”

Section: Assaysmentioning

confidence: 99%

Thyroxine treatment increases circulating levels of insulin‐like growth factor binding protein‐1: a placebo‐controlled study

Angervo

Tiihonen²,

Leinonen³

et al. 1993

Clinical Endocrinology

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Time-resolved immunofluorometric assay of 34-kDa somatomedin-binding protein.

Cited by 39 publications

References 0 publications

Diet‐induced Changes in Sex Hormone Binding Globulin and Free Testosterone in Women With Normal or Polycystic Ovaries: Correlation With Serum Insulin and Insulin‐like Growth Factor‐i

Diet‐induced Changes in Sex Hormone Binding Globulin and Free Testosterone in Women With Normal or Polycystic Ovaries: Correlation With Serum Insulin and Insulin‐like Growth Factor‐i

Serum levels of endometrial proteins during transcervical resection of the endometrium

Thyroxine treatment increases circulating levels of insulin‐like growth factor binding protein‐1: a placebo‐controlled study

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