Time-resolved FTIR difference spectroscopy for the study of photosystem I with high potential naphthoquinones incorporated into the A1 binding site 2: Identification of neutral state quinone bands
Abstract:Time-resolved step-scan FTIR difference spectroscopy at 77 K has been used to study cyanobacterial photosystem I (PSI) from Synechocystis sp. PCC 6803 with four high-potential, 1,4-naphthoquinones (NQs) incorporated into the A 1 binding site. The incorporated quinones are 2-chloro-NQ (2ClNQ), 2bromo-NQ (2BrNQ), 2,3-dichloro-NQ (Cl 2 NQ), and 2,3-dibromo-NQ (Br 2 NQ). For completeness 2methyl-NQ (2MNQ) was also incorporated and studied. Here we focus on spectra in the previously unexplored 1400 − 1200 cm − 1 sp… Show more
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