Time‐Resolved FRET Biosensor Based on Amine‐Functionalized Lanthanide‐Doped NaYF<sub>4</sub> Nanocrystals

Tu, Datao; Liu, Liqin; Ju, Qiang; Liu, Yongsheng; Zhu, Haomiao; Li, Renfu; Chen, Xueyuan

doi:10.1002/anie.201100303

Cited by 312 publications

(180 citation statements)

References 57 publications

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“…[11][12][13] For in vivo bioapplications, upconversion nanoparticles that are excitable at 980 nm have previously been the focus. This is because this excitation wavelength falls in the so-called "optical window" of tissue and the biological environment is hardly excited, leading to high quality as well as relatively deep depth imaging.…”

Section: Introductionmentioning

confidence: 99%

808 nm driven Nd³⁺-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging

et al. 2015

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UvA-DARE is a service provided by the library of the University of Amsterdam (http://dare.uva.nl) General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). UvA-DARE (Digital AcademicRepository Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. The in vivo biological applications of upconversion nanoparticles (UCNPs) prefer excitation at 700-850 nm, instead of 980 nm, due to the absorption of water. Recent approaches in constructing robust Nd 3+ doped UCNPs with 808 nm excitation properties rely on a thick Nd 3+ sensitized shell.However, for the very important and popular Förster resonance energy transfer (FRET)-based applications, such as photodynamic therapy (PDT) or switchable biosensors, this type of structure has restrictions resulting in a poor energy transfer. In this work, we have designed a NaYF 4 :Yb/Ho@NaYF 4 :Nd@NaYF 4 core-shell-shell nanostructure. We have proven that this optimal structure balances the robustness of the upconversion emission and the FRET efficiency for FRET-based bioapplications. A proof of the concept was demonstrated for photodynamic therapy and simultaneous fluorescence imaging of HeLa cells triggered by 808 nm light, where low heating and a high PDT efficacy were achieved.

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Section: Introductionmentioning

confidence: 99%

808 nm driven Nd³⁺-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging

et al. 2015

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“…UCNPs are conjugated with energy acceptors, whose excitation spectra has to overlap with the emission spectra of the NPs. The acceptor can be organic fluorophores [111,144,344,345], fluorescent proteins [346], metallic NPs [341], graphene oxide (GO) [338,339,347,348], carbon NPs [342] and semiconductor QDs [345,349,350]. The working principle of upconversion LRET [depicted in Figure 21(i)] has demonstrated its huge potential through the development of sensing systems for the determination of 17β-estradiol [336], enzymatic activity [351], matrix metalloproteinase [342], mycotoxins [338], ATP [339], IgG [352], glucose [347] and pesticides [353], among others.…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Rare earth based nanostructured materials: synthesis, functionalization, properties and bioimaging and biosensing applications

et al. 2017

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Abstract:Rare earth based nanostructures constitute a type of functional materials widely used and studied in the recent literature. The purpose of this review is to provide a general and comprehensive overview of the current state of the art, with special focus on the commonly employed synthesis methods and functionalization strategies of rare earth based nanoparticles and on their different bioimaging and biosensing applications. The luminescent (including downconversion, upconversion and permanent luminescence) and magnetic properties of rare earth based nanoparticles, as well as their ability to absorb X-rays, will also be explained and connected with their luminescent, magnetic resonance and X-ray computed tomography bioimaging applications, respectively. This review is not only restricted to nanoparticles, and recent advances reported for in other nanostructures containing rare earths, such as metal organic frameworks and lanthanide complexes conjugated with biological structures, will also be commented on.

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“…In a typical TR-FRET process, the energy transfer from Ln 3+ -NP donor will apparently lengthen the PL lifetime of the acceptor such as organic dyes that are intrinsically short-lived, due to the slow population of the acceptor's excited-state from the long-lived Ln 3+ 's excited state [27]. When the TR technique is applied, the PL of the acceptor lengthened by the FRET process can be readily distinguished from their intrinsically short-lived PL co-excited under UV excitation.…”

Section: Time-resolved Luminescent Bioassaymentioning

confidence: 99%

“…Such a signal screening guarantees both reliability and sensitivity of the assay. By employing biotinylated NaYF 4 :Ce,Tb NPs as an energy donor and fluorescein isothiocyanate (FITC)-labeled avidin as an energy acceptor, we constructed the first TR-FRET pair for the detection of avidin with an LOD of ~4.8 nM [27]. Later on, this novel TR-FRET technique was refined and extended to other Ln 3+ -NPs such as KGdF 4 : Tb 3+ and ZrO 2 : Tb 3+ NPs for avidin assays with LODs of ~5.5 nM and ~3.0 nM, respectively [102,111].…”

Section: Time-resolved Luminescent Bioassaymentioning

confidence: 99%

“…Therefore, they are emerging as promising next-generation luminescent nano-bioprobes for versatile biomedical applications. Particularly, the long-lived downshifting luminescence (DSL) and high photochemical stability of Ln 3+ -doped NPs make them ideal alternative to Ln 3+ -chelates for TRPL biodetection to eliminate the short-lived background noise from scattered lights and autofluorescence from the biological samples [26,27]. Such unwanted background noise can also be overcome by using the unique anti-Stokes upconverting luminescence (UCL) and persistent luminescence of Ln This review summarizes the most recent advances in the development of inorganic lanthanide nano-bioprobes and their salient applications for background-free luminescent bioassays.…”

Section: Introductionmentioning

confidence: 99%

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Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Huang

Zheng

et al. 2015

Sci. China Mater.

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Luminescent bioassay techniques have been widely adopted in a variety of research and medical institutions. However, conventional luminescent bioassays utilizing traditional bioprobes like organic dyes and quantum dots often suffer from the interference of background noise from scattered lights and autofluorescence from biological matrices. To eliminate this disadvantage, the use of inorganic lanthanide (Ln 3+ )-doped nanoparticles (NPs) is an excellent option in view of their superior optical properties, such as the long-lived downshifting luminescence, near-infrared triggered anti-Stokes upconverting luminescence and excitation-free persistent luminescence. In this review, we summarize the latest advances in the development of inorganic Ln 3+ -doped NPs as sensitive luminescent bioprobes from their fundamental physicochemical properties to biodetection, including the chemical synthesis, surface functionalization, optical properties and their promising applications for background-free luminescent bioassays. Future efforts and prospects towards this rapidly growing field are also proposed. INTRODUCTIONSensitive and specific bioassay of trace amount of target analytes is essential for a variety of biomedical applications ranging from pharmaceutical preparation to disease diagnosis and therapy [1][2][3]. Among various bioassay methods, luminescent bioassay has received particular attention because of its high sensitivity and good specificity [4,5]. Conventional luminescent bioassay techniques such as enzyme-linked immunosorbent assay (ELISA), time-resolved (TR) fluoroimmunoassay (TRFIA), Förster resonance energy transfer (FRET) and TR-FRET assays have laid the foundation for many modern clinical applications [6][7][8][9][10][11]. However, these bioassays are impeded by the availability of traditional bioprobes like organic dyes, lanthanide (Ln 3+ ) chelates and quantum dots (QDs). The use of these bioprobes has a number of limitations. Organic dyes commonly possess poor photochemical stability and suffer from serious photobleaching [12]. The applicability of QDs is compromised by photoblinking and high toxic- ity of heavy metal elements like cadmium and selenium, especially for in vivo biosensing [13,14]. Moreover, both organic dyes and QDs may induce high background noise and considerable photodamage to the biological samples under ultraviolet (UV) excitation, which deteriorates their detection sensitivity for bioassays [15,16]. Although such background noise can be suppressed by the technique of TR photoluminescence (PL) through the use of the longlived luminescence of Ln 3+ -chelates, the poor photochemical stability, long-term toxicity and high cost of Ln 3+ -chelates remain a major issue [17]. These concerns fuel a crucial demand for the development of a new generation of luminescent bioprobes to circumvent the limitations of traditional ones.Recently, with the explosion of nanoscience and nanotechnology, there is a growing dedication towards the development of diverse luminescent nanoprobes for biodetection ...

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Time‐Resolved FRET Biosensor Based on Amine‐Functionalized Lanthanide‐Doped NaYF₄ Nanocrystals

Cited by 312 publications

References 57 publications

808 nm driven Nd³⁺-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging

808 nm driven Nd³⁺-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging

Rare earth based nanostructured materials: synthesis, functionalization, properties and bioimaging and biosensing applications

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

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Time‐Resolved FRET Biosensor Based on Amine‐Functionalized Lanthanide‐Doped NaYF4 Nanocrystals

Cited by 312 publications

References 57 publications

808 nm driven Nd3+-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging

808 nm driven Nd3+-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging

Rare earth based nanostructured materials: synthesis, functionalization, properties and bioimaging and biosensing applications

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Contact Info

Product

Resources

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Time‐Resolved FRET Biosensor Based on Amine‐Functionalized Lanthanide‐Doped NaYF₄ Nanocrystals

808 nm driven Nd³⁺-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging

808 nm driven Nd³⁺-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging