Abstract:A procedure for the selective fractionation of the bone and liver alkaline phosphatase
activity in tissue extracts and human sera is proposed. Optimized conditions of the
assay are: urea 3.7 mol/1 in 0.5 mol/1 DEA buffer, pH 9.8; 0.5 mmol/1 MgCl(2); 10.0 mmol/1
p-nitrophenyl phosphate. The sample is diluted 1:20 in the reagent solution and the activity
is recorded for 10 min at 37 °C. By means of a computerized or manual graphic analysis,
based on ‘peeling-off’ the exponentials, the two differently urea-sensit… Show more
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