Time-resolved fluorometric immunoassay for rubella antibody—a useful method for serosurveillance studies

Maple, P. A. C.; Jones, C S

doi:10.1016/s0264-410x(01)00474-1

Cited by 8 publications

(1 citation statement)

References 11 publications

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“…Further studies are needed to confirm the utility of an indirect EIA format for rubella IgG detection in oral fluids and it will be seen in Section 3.3 that time-resolved fluorescence immunoassay can offer the sensitivity required. A rubella IgG time-resolved fluorescence immunoassay has been described [ 82 ] with a lower limit of detection of 0.2 IU/mL in sera and initial results on application to oral fluid appear promising.…”

Section: Rubella: Rubella Vaccination and Rubella Oral Fluid Testimentioning

confidence: 99%

Application of Oral Fluid Assays in Support of Mumps, Rubella and Varicella Control Programs

Maple

2015

Vaccines

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Detection of specific viral antibody or nucleic acid produced by infection or immunization, using oral fluid samples, offers increased potential for wider population uptake compared to blood sampling. This methodology is well established for the control of HIV and measles infections, but can also be applied to the control of other vaccine preventable infections, and this review describes the application of oral fluid assays in support of mumps, rubella and varicella national immunization programs. In England and Wales individuals with suspected mumps or rubella, based on clinical presentation, can have an oral fluid swab sample taken for case confirmation. Universal varicella immunization of children has led to a drastic reduction of chickenpox in those countries where it is used; however, in England and Wales such a policy has not been instigated. Consequently, in England and Wales most children have had chickenpox by age 10 years; however, small, but significant, numbers of adults remain susceptible. Targeted varicella zoster virus (VZV) immunization of susceptible adolescents offers the potential to reduce the pool of susceptible adults and oral fluid determination of VZV immunity in adolescents is a potential means of identifying susceptible individuals in need of VZV vaccination. The main application of oral fluid testing is in those circumstances where blood sampling is deemed not necessary, or is undesirable, and when the documented sensitivity and specificity of the oral fluid assay methodology to be used is considered sufficient for the purpose intended.

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Section: Rubella: Rubella Vaccination and Rubella Oral Fluid Testimentioning

confidence: 99%

Application of Oral Fluid Assays in Support of Mumps, Rubella and Varicella Control Programs

Maple

2015

Vaccines

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Collection tubes with or without gel separator did not interfere with detection of rubella virus antibodies IgM and IgG

Oliveira

Kawasato

Otta

et al. 2007

Clinical Laboratory Analysis

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Rubella infection is an exanthematic disease, with high prevalence in the adult population. The only modality of disease that causes serious consequences is congenital rubella syndrome (CRS), which happens when a pregnant woman seronegative to rubella virus acquires the infection during early pregnancy. Due to the lack of signals and characteristic symptoms of disease, diagnosis of rubella is based essentially on laboratory tests: antibodies detection and/or virus isolation. Results of serologic tests should always be interpreted with caution, because they can be affected by the quality of blood samples, processing and storage of sera, the equipment and reagents used to perform tests, and finally by the technical expertise and training of biologists. The collection tubes with gel seem to facilitate serum separation, but on the other hand gels can retain and consequently decrease antibody titers. Therefore, we decided to investigate whether the use of collection tubes containing gel separator might interfere with rubella virus antibody detection in blood samples from children. We did not observe statistically significant differences with respect to rubella virus antibody detection (immunoglobulin M [IgM] and immunoglobulin G [IgG]) for samples collected in tubes with or without gel separator, from the two evaluated manufacturers.

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Salivary Diagnosis of Infectious Diseases

Martı́nez-Subiela

Escribano

2020

Saliva in Health and Disease

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Time-resolved fluorometric immunoassay for rubella antibody—a useful method for serosurveillance studies

Cited by 8 publications

References 11 publications

Application of Oral Fluid Assays in Support of Mumps, Rubella and Varicella Control Programs

Application of Oral Fluid Assays in Support of Mumps, Rubella and Varicella Control Programs

Collection tubes with or without gel separator did not interfere with detection of rubella virus antibodies IgM and IgG

Salivary Diagnosis of Infectious Diseases

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