Time-resolved fluorometer for lanthanide chelates--a new generation of nonisotopic immunoassays.

Abstract

Pulsed-light time-resolved fluorometry of lanthanide chelates has proved to be very sensitive for use with nonisotopic immunoassays. We describe a manually operated fluorometer with a conventional xenon flashtube. Sensitivity for 1-s determinations is similar to that of radioisotopic methods.

Serum leptin concentrations in a normal population and in GH deficiency: negative correlation with testosterone in men and effects of GH treatment

“…Serum Cpeptide was analysed by RIA (Lindström et al, 1992) and intraand interassay coefficients of variation were 7% and 8%, respectively. Serum testosterone was determined by fluoroimmunoassay (Soini & Kojola 1983) and intra-and interassay coefficients of variation were 6% and 5%, respectively. Serum SHBG was analysed by RIA (Fernlund et al, 1985) and intraand interassay coefficients of variation were 4% and 5%, respectively.…”

Time-resolved fluorimetry in the study of attachment of staphylococci and streptococci on substrate-bound fibronectin

“…Time-resolved fluorimetry is a new technique based on fluorescence produced by a chelate of a fluorochrome and a rare earth lanthanide, europium (Eu; for reviews, see [19,20]). Fluorescence is measured after a short delay, which eliminates the effect of scattering from excitation radiation.…”

16 Methods for Detecting Microbial Pathogens in Food and Water

“…Alternative fluorescent labels to those mentioned above, although not commonly used in microbiology, include lanthanide elements like europium and terbium chelates (Soini and Hemmila, 1979;Soini and Kojola, 1983). The advantages of using these compounds are the high quantum yield and narrow emission peaks produced upon excitation.…”