Time-resolved fluoroimmunoassays of the complete set of secreted phospholipases A2 in human serum

Nevalainen, Timo J.; Eerola, Leena I.; Rintala, Esa; Laine, V.; Lambeau, Gérard; Gelb, Michael H.

doi:10.1016/j.bbalip.2004.12.012

Cited by 75 publications

(70 citation statements)

References 55 publications

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“…2a)). We also searched for the presence of other sPLA2s in CF expectorations by TRFIA method 24 . We detected only sPLA2-IIA and sPLA2-X ( Supplementary Fig.…”

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confidence: 99%

Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity

et al. 2014

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Young cystic fibrosis (CF) patients' airways are mainly colonized by Staphylococcus aureus, while Pseudomonas aeruginosa predominates in adults. However, the mechanisms behind this infection switch are unclear. Here, we show that levels of type-IIA-secreted phospholipase A2 (sPLA2-IIA, a host enzyme with bactericidal activity) increase in expectorations of CF patients in an age-dependent manner. These levels are sufficient to kill S. aureus, with marginal effects on P. aeruginosa strains. P. aeruginosa laboratory strains and isolates from CF patients induce sPLA2-IIA expression in bronchial epithelial cells from CF patients (these cells are a major source of the enzyme). In an animal model of lung infection, P. aeruginosa induces sPLA2-IIA production that favours S. aureus killing. We suggest that sPLA2-IIA induction by P. aeruginosa contributes to S. aureus eradication in CF airways. Our results indicate that a bacterium can eradicate another bacterium by manipulating the host immunity.

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“…2a)). We also searched for the presence of other sPLA2s in CF expectorations by TRFIA method 24 . We detected only sPLA2-IIA and sPLA2-X ( Supplementary Fig.…”

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confidence: 99%

Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity

et al. 2014

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“…sPLA 2 can destroy bacteria through hydrolysis of their membrane lipids, and disturb surfactant function through hydrolysis of their phospholipid bilayer. 8,10,11 In adults, sPLA 2 is proposed as a sensitive marker for sepsis, and increased plasma levels of sPLA 2 are associated with development of multiple organ failure, vascular dilatation during shock and death. 6,7,12 The role of sPLA 2 in neonatal sepsis is unknown and its levels and relationship with well-recognized markers of inflammation have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

“…These substances are involved in inflammatory processes. 8,9 cPLA 2 mainly controls intracellular eicosanoid metabolism. sPLA 2 is an acute phase protein which plays a major role in inflammatory diseases by controlling extracellular eicosanoid production.…”

Section: Introductionmentioning

confidence: 99%

“…9 In humans, cytosolic PLA 2 (cPLA 2 ), calcium 2 þ -independent PLA 2 and secretory PLA 2 (sPLA 2 ) forms of PLA 2 have been identified. 8,9 PLA 2 catalyzes the rate-limiting step in formation of inflammatory lipid mediators, for example, eicosanoids and platelet activating factor, by hydrolyzing membrane phospholipids and releasing their precursors arachidonic acid (AA) and a lysophospholipid. AA is metabolized by cyclo-oxygenase and lipoxygenase enzymatic pathways to produce eicosanoids, including prostaglandins and leukotrienes.…”

Section: Introductionmentioning

confidence: 99%

“…4,5 Phospholipase A 2 (PLA 2 ) may be an attractive candidate for study since its plasma concentration and activity is increased in adults with sepsis. [6][7][8] Several new PLA 2 inhibitors are currently being studied in adults with sepsis. 9 In humans, cytosolic PLA 2 (cPLA 2 ), calcium 2 þ -independent PLA 2 and secretory PLA 2 (sPLA 2 ) forms of PLA 2 have been identified.…”

Section: Introductionmentioning

confidence: 99%

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Secretory phospholipase A2 in newborn infants with sepsis

et al. 2008

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Objective: To investigate secretory phospholipase A 2 (sPLA 2 ) activity in neonatal sepsis.Study Design: Plasma sPLA 2 activity, C-reactive protein (CRP) concentration, leukocyte count and immature/total neutrophil (I/T) ratio were assessed in a group of 156 infants admitted for neonatal intensive care, who were classified as documented sepsis (n ¼ 24), suspected infection (n ¼ 77) and controls (n ¼ 55). Interleukin-6 (IL-6) concentrations were assessed in a subgroup (n ¼ 29).Result: sPLA 2 activity, CRP concentration and I/T ratio were higher in sepsis than in suspected infection or control groups. sPLA 2 activity advanced with increasing CRP, I/T ratio and IL-6 was highest in infants with respiratory distress syndrome (RDS). Compared to CRP, sPLA 2 had equal sensitivity and lower specificity. Compared to I/T ratio, sensitivity and specificity of sPLA 2 were higher.Conclusion: Plasma sPLA 2 activity is increased in neonatal sepsis and highest in infants with RDS. Further studies should assess the potential of sPLA 2 inhibition in neonatal sepsis.

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Risk of Toxicity Associated with Unregulated Herbal Products

Cotten

2010

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Time-resolved fluoroimmunoassays of the complete set of secreted phospholipases A2 in human serum

Cited by 75 publications

References 55 publications

Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity

Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity

Secretory phospholipase A2 in newborn infants with sepsis

Risk of Toxicity Associated with Unregulated Herbal Products

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