Time-resolved fluorescence resonance energy transfer-based lateral flow immunoassay using a raspberry-type europium particle and a single membrane for the detection of cardiac troponin I

Lee, Kyungwon; Kim, Ka Ram; Chun, Hyeong Jin; Jeong, Kwan Young; Hong, Dae Ki; Lee, Kook-Nyung; Yoon, Hyun C.

doi:10.1016/j.bios.2020.112284

Cited by 60 publications

(30 citation statements)

References 26 publications

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“…The electrochemical properties of the sensor was further investigated by EIS. In EIS, semicircular and straight regions indicate electron transfer-limited processes and mass transfer control processes (Demirbakan and Kemal Sezgintürk, 2020;Lee et al, 2020), respectively. Considering the variation of the electron transfer resistance (R ct ), the EIS method can provide important information to demonstrate the interaction between the aptamer and the target protein.…”

Section: Resultsmentioning

confidence: 99%

A Novel Graphene-Based Nanomaterial Modified Electrochemical Sensor for the Detection of Cardiac Troponin I

Zhang

et al. 2021

Front. Chem.

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Acute myocardial infarction has a high clinical mortality rate. The initial exclusion or diagnosis is important for the timely treatment of patients with acute myocardial infarction. As a marker, cardiac troponin I (cTnI) has a high specificity, high sensitivity to myocardial injury and a long diagnostic window. Therefore, its diagnostic value is better than previous markers of myocardial injury. In this work, we propose a novel aptamer electrochemical sensor. This sensor consists of silver nanoparticles/MoS2/reduced graphene oxide. The combination of these three materials can provide a synergistic effect for the stable immobilization of aptamer. Our proposed aptamer electrochemical sensor can detect cTnl with high sensitivity. After optimizing the parameters, the sensor can provide linear detection of cTnl in the range of 0.3 pg/ml to 0.2 ng/ml. In addition, the sensor is resistant to multiple interferents including urea, glucose, myoglobin, dopamine and hemoglobin.

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Section: Resultsmentioning

confidence: 99%

A Novel Graphene-Based Nanomaterial Modified Electrochemical Sensor for the Detection of Cardiac Troponin I

Zhang

et al. 2021

Front. Chem.

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“…More importantly, LOD reached a value as low as 17 pg mL −1 . Compared with other reports related to cTnI determination (Table 1), 13,[37][38][39][40][41][42][43][44][45] the prepared lanthanide-doped NPs-based LFA has the merits of relatively low LOD and wide linear range. As for colorimetry, it is hard to distinguish the intermediate color with the naked eye.…”

Section: Analytical Performance Of Lfa Platformmentioning

confidence: 86%

Highly Sensitive Lanthanide-Doped Nanoparticles-Based Point-of-Care Diagnosis of Human Cardiac Troponin I

Chen¹,

Zhou²,

Zhu³

et al. 2022

IJN

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Cardiac troponin I (cTnI) has been regarded as a gold standard for early diagnosis and prognosis monitoring of acute myocardial infarction (AMI) in clinical practice. Owing to its low concentration in blood, accurate determination of cTnI often requires high sensitivity. However, current established point-of-care (POC) assays are insufficient to meet clinically analytical requirements due to their low sensitivity. Methods: To this end, we established a highly sensitive and reliable POC lateral flow strip based on lanthanide-doped nanoparticles (NPs) for cTnI determination in human blood samples. The capture of cTnI on the lateral flow strip was performed in a sandwich assay, where Eu 3+ -doped vanadate nanoparticles (GdVO 4 :30% Eu NPs) were used as luminescent probes to allow quantification. Results: Our platform realized the analytical sensitivity enhancement with limit-of-detection (LOD) as low as 17 pg mL −1 for cTnI detection, which was lower than the commercial counterpart; meanwhile, it displayed high specificity, excellent reproducibility and outstanding accuracy for analyzing clinical serum samples. Conclusion: Overall, this strategy provided an ultrasensitive, cost-effective and user-friendly platform for on-site cTnI detection, demonstrating the prospect of lanthanide-doped NPs-based POC diagnosis of disease-related biomarkers.

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“…LFA can use different materials to label antibody such as latex beads [11], carbon nanoparticles [12], colloidal silver [13], dye-loaded liposomes [14], gold nanoparticles (AuNPs) [7], europium chelate-loaded silica nanoparticles [15], quantum dots [16] and upconverting phosphors [17]. Each label is able to create different characteristics in the diagnostic platform, but the main purpose in using different labels is to try to make a more sensitive assay.…”

Section: Design and Principle Of Lateral Flow Assaysmentioning

confidence: 99%

Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests

2022

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The lateral flow assay (LFA) is an extensively used paper-based platform for the rapid and on-site detection of different analytes. The method is user-friendly with no need for sophisticated operation and only includes adding sample. Generally, antibodies are employed as the biorecognition elements in the LFA. However, antibodies possess several disadvantages including poor stability, high batch-to-batch variation, long development time, high price and need for ethical approval and cold chain. Because of these limitations, aptamers screened by an in vitro process can be a good alternative to antibodies as biorecognition molecules in the LFA. In recent years, aptamer-based LFAs have been investigated for the detection of different analytes in point-of-care diagnostics. In this review, we summarize the applications of aptamer technology in LFAs in clinical diagnostic rapid tests for the detection of biomarkers, microbial analytes, hormones and antibiotics. Performance, advantages and drawbacks of the developed assays are also discussed.

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Time-resolved fluorescence resonance energy transfer-based lateral flow immunoassay using a raspberry-type europium particle and a single membrane for the detection of cardiac troponin I

Cited by 60 publications

References 26 publications

A Novel Graphene-Based Nanomaterial Modified Electrochemical Sensor for the Detection of Cardiac Troponin I

A Novel Graphene-Based Nanomaterial Modified Electrochemical Sensor for the Detection of Cardiac Troponin I

Highly Sensitive Lanthanide-Doped Nanoparticles-Based Point-of-Care Diagnosis of Human Cardiac Troponin I

Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests

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