Time-resolved fluorescence immunoassay (TRFIA) for the detection of Escherichia coli O157:H7 in apple cider

Yu, Linda; Reed, Sandra M.; Golden, M.H.

doi:10.1016/s0167-7012(01)00352-9

Cited by 52 publications

(23 citation statements)

References 19 publications

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“…For ground beef, the detection limit was 1 CFU 10 g −1 after enrichment for 7 h while for apple cider it was 0·5 CFU ml −1 after a 7‐h enrichment. In a similar approach, a time‐resolved fluorescence immunoassay (TRFIA) using IMS was used to detect organisms in apple cider (Yu et al. 2002).…”

Section: 22 Nonenzymatic Immunoassaysmentioning

confidence: 99%

Strategies for the detection of Escherichia coli O157:H7 in foods

2004

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Section: 22 Nonenzymatic Immunoassaysmentioning

confidence: 99%

Strategies for the detection of Escherichia coli O157:H7 in foods

2004

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“…This compares favorably to the limits of detection for other tests (21,26,47). Since enrichment is a prerequisite for using many immunosensors (21,31,35,47), the AICC assay is a more viable alternative as no enrichment is required for this assay to achieve low detection limits. Furthermore, large sample volumes can be applied, and thus lower concentrations of cells can be detected by concentrating the analyte (18).…”

Section: Discussionmentioning

confidence: 94%

Rapid Affinity Immunochromatography Column-Based Tests for Sensitive Detection of Clostridium botulinum Neurotoxins and Escherichia coli O157

Brunt

Webb

Peck

2010

Appl Environ Microbiol

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Existing methods for detection of food-borne pathogens and their toxins are frequently time-consuming, require specialized equipment, and involve lengthy culture procedures and/or animal testing and are thus unsuitable for a rapid response to an emergency public health situation. A series of simple and rapid affinity immunochromatography column (AICC) assays were developed to detect Clostridium botulinum neurotoxin types A, B, E, and F and Escherichia coli O157 in food matrices. Specifically, for milk, grape juice with peach juice, and bottled water, the detection limit for the botulinum neurotoxin type A complex was 0.5 ng. Use of this method with a 10-ml sample would therefore result in a detection limit of 50 pg ml ؊l . Thus, this assay is approximately 2 orders of magnitude more sensitive than a comparable lateral-flow assay. For botulinum neurotoxin complex types B, E, and F, the minimum detection limit was 5 ng to 50 ng. Sensitive detection of E. coli O157 was achieved, and the detection limit was 500 cells. The AICC test was also shown to be specific, rapid, and user friendly. This test takes only 15 to 30 min to complete without any specialized equipment and thus is suitable for use in the field. It has the potential to replace existing methods for presumptive detection of botulinum neurotoxin types A, B, E, and F and E. coli O157 in contaminated matrices without a requirement for preenrichment.

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“…The principles and advantages of TRF have been described in numerous works (Tu et al 2003a(Tu et al , 2007Yu et al 2002Yu et al , 2003. TRF utilizes lanthanide chelate labels, which produce intense fluorescent signals.…”

Section: Introductionmentioning

confidence: 99%

Detection of Salmonella Enteriditis from Egg Components Using Different Immunomagnetic Beads and Time-resolved Fluorescence

Reed

Gehring

et al. 2008

Food Anal. Methods

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The types of chemical linkage used to bind antibodies to magnetic beads to form immunomagnetic beads (IMB) were compared in the capture and detection of Salmonella Enteriditis from egg white, egg yolk, and whole egg. Egg components were inoculated with outbreak strains of S. Enteriditis. After incubation under different conditions, IMBs derived from linking antibodies to core magnetic beads via biotin-streptavidin interactions, Schiffbase bonds and unspecified proprietary chemistry were used to capture S. Enteriditis. Europium-labeled antiSalmonella antibodies completed the sandwich, and timeresolved fluorescence served as the means of detection. For the Salmonella isolated in stationary phase and cultured from universal pre-enrichment broth (UPB), the detection signal intensity was affected by the chemistry utilized to link the antibodies to IMB, with results varying among the three test strains. When S. Enteriditis was cultured in egg yolk alone, plating data were similar to those of the growth of S. Enteriditis in UPB. Egg white by itself did not support the growth of S. Enteriditis. The addition of UPB to egg white restored the growth of Salmonella and yielded stronger detection signals than from cultures obtained from UPB with egg yolk. The detection signals obtained from the immunoassay were less intense for cultures grown in egg yolk + UPB than from cultures grown in UPB alone. The lower detection signals elicited by all IMBs suggest the availability of the antigenic groups recognized by the antibodies on IMBs was reduced in the presence of egg yolk.

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Time-resolved fluorescence immunoassay (TRFIA) for the detection of Escherichia coli O157:H7 in apple cider

Cited by 52 publications

References 19 publications

Strategies for the detection of Escherichia coli O157:H7 in foods

Strategies for the detection of Escherichia coli O157:H7 in foods

Rapid Affinity Immunochromatography Column-Based Tests for Sensitive Detection of Clostridium botulinum Neurotoxins and Escherichia coli O157

Detection of Salmonella Enteriditis from Egg Components Using Different Immunomagnetic Beads and Time-resolved Fluorescence

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