Time-resolved detection probe for homogeneous nucleic acid analyses in one-step format

Laitala, Ville; Ylikoski, Alice; Raussi, Hanna-Mari; Ollikka, Pia; Hemmilä, Ilkka

doi:10.1016/j.ab.2006.11.015

Cited by 26 publications

(29 citation statements)

References 15 publications

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“…In unhybridized free form, flexibility of the probe allows very efficient energy transfer resulting in very short-lived sensitized signal. Upon hybridization, the energy transfer is decreased and decay is longer [43]. Figure 5 shows the assay principle and the respective decays in an assay.…”

Section: Gated Detection and Signal Processingmentioning

confidence: 96%

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Sensitized Bioassays

Hemmilä¹,

Laitala²

2010

Lanthanide Luminescence

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Luminescent lanthanides are well-suited and widely applied markers for bioaffinity assays, both separation-based heterogeneous assays, such as immunoassays, as well as for homogeneous assays, such as assays based on energy transfer. Their properties allow efficient combination of spectral and temporal resolution to gain good sensitivity, perfect discrimination of produced sensitized signal, and robust assays. Both europium and terbium, as their stable and highly luminescent chelates, have found wide applications in research aiming for new drug discovery. The complicated but efficient energy transfer process in the chelates, and between chelate and acceptor, provides versatile tools to stipulate good assays for various applications and also to gain limited multiplexing to allow measurement of several biomarkers simultaneously. The chapter reviews the recent development in the area and gives overview of interesting applications.

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Section: Gated Detection and Signal Processingmentioning

confidence: 96%

“…Doubly labeled beacon-type probes containing europium chelate as donor and either Alexa 647 or Alexa Fluor 700 as acceptors has been utilized by measuring both the wavelength and decay change upon hybridization [43].…”

Section: Multiplexingmentioning

confidence: 99%

Sensitized Bioassays

Hemmilä¹,

Laitala²

2010

Lanthanide Luminescence

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“…Much research is directed towards the development of specific fluorophores such as the green fluorescent protein (GFP) or quantum dots [60] and thanks to the progress within the field, fluorescence microscopy is currently experiencing a boom. Equally important is the development of sensitive and fast detectors to record signals from single molecules even at the picosecond level [64][65][66] as well as new filtering techniques in order to precisely sort out minute energy differences [67][68][69][70]. Widely used fluorescent techniques include wide-field fluorescence microscopy, confocal laser scanning microscopy and multiphoton microscopy.…”

Section: Combination Of Optical Manipulation Techniques With Microscomentioning

confidence: 99%

Optical manipulation for single‐cell studies

Ramser

Hanstorp

2010

Journal of Biophotonics

100

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In the last decade optical manipulation has evolved from a field of interest for physicists to a versatile tool widely used within life sciences. This has been made possible in particular due to the development of a large variety of imaging techniques that allow detailed information to be gained from investigations of single cells. The use of multiple optical traps has high potential within single-cell analysis since parallel measurements provide good statistics. Multifunctional optical tweezers are, for instance, used to study cell heterogeneity in an ensemble, and force measurements are used to investigate the mechanical properties of individual cells. Investigations of molecular motors and forces on the single-molecule level have led to discoveries that would have been difficult to make with other techniques. Optical manipulation has prospects within the field of cell signalling and tissue engineering. When combined with microfluidic systems the chemical environment of cells can be precisely controlled. Hence the influence of pH, salt concentration, drugs and temperature can be investigated in real time. Fast advancing technical developments of automated and user-friendly optical manipulation tools and cross-disciplinary collaboration will contribute to the routinely use of optical manipulation techniques within the life sciences.

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“…Thus, a binding event that brings an acceptor in close proximity to the donor results in a reduction in the donor's lifetime. FRET efficiency is sensitive to the local environments of donors and acceptors, allowing the method to be applicable in many areas such as genotyping, [74][75][76] nucleic acids detection, 77 and probing DNAs with complicated structures. 78,79 McGuinness and co-workers used a DNA Holliday junction nanoswitch labeled with FAM and TAMRA to discriminate SNPs.…”

Section: Fluorescence-lifetime-based Biosensingmentioning

confidence: 99%

Nucleic Acid Fluorescent Probes for Biological Sensing

Xiao

Zhang

et al. 2012

Appl Spectrosc

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Nucleic acid fluorescent probes are playing increasingly important roles in biological sensing in recent years. In addition to the conventional functions of single-stranded DNA/RNA to hybridize with their complementary strands, affinity nucleic acids (aptamers) with specific target binding properties have also been developed, which has greatly broadened the application of nucleic acid fluorescent probes to the detection of a large variety of analytes, including small molecules, proteins, ions, and even whole cells. Another chemical property of nucleic acids is to act as substrates for various nucleic acid enzymes. This property can be utilized not only to detect those enzymes and screen their inhibitors, but also employed to develop effective signal amplification systems, which implies extensive applications. This review mainly covers the biosensing methods based on the above three types of nucleic acid fluorescent probes. The most widely used intensity-based biosensing assays are covered first, including nucleic acid probe-based signal amplification methods. Then fluorescence lifetime, fluorescence anisotropy, and fluorescence correlation spectroscopy assays are introduced, respectively. As a rapidly developing field, fluorescence imaging approaches are also briefly summarized.

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Time-resolved detection probe for homogeneous nucleic acid analyses in one-step format

Cited by 26 publications

References 15 publications

Sensitized Bioassays

Sensitized Bioassays

Optical manipulation for single‐cell studies

Nucleic Acid Fluorescent Probes for Biological Sensing

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