Time-resolved detection of lanthanide luminescence for ultrasensitive bioanalytical assays

Dickson, Eva F. Gudgin; Pollak, Alfred; Diamandis, Eleftherios P.

doi:10.1016/1011-1344(94)07086-4

Cited by 169 publications

(59 citation statements)

References 97 publications

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“…[5,17] This is different to the La III -TTHA system, where the 1 H and 13 C NMR spectra revealed the existence of only one coordination arrangement around the metal cation in solution. [5] Our results based on the optical properties of Nd III , [8,16] Eu III [17,18] and Ho III [13] complexes with TTHA revealed that species differing in the number of coordinated nitrogen and oxygen atoms, namely [Ln(N 4 3-, are present in the solution for the light and heavy lanthanide ions, respectively. Each of these species may form various stereoisomers, some of which may be op-tically active.…”

Section: Ndmentioning

confidence: 91%

“…The wide variety of biomedical applications of lanthanide complexes with polyamino polycarboxylic acids, including their use as contrast agents in MRI [1] and X-ray [2] diagnosis, NMR hyperfine shift-reagents [3] and bioanalytical assays, [4] is the reason for their being extensively investigated both in solution and in the solid state. The principal aim of these studies is to understand the physicochemical properties of these compounds in solution.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ten‐Coordinate Neodymium(III) Complexes with Triethylenetetraaminehexaacetic Acid

Mondry

Starynowicz

2006

Eur J Inorg Chem

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Section: Ndmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Ten‐Coordinate Neodymium(III) Complexes with Triethylenetetraaminehexaacetic Acid

Mondry

Starynowicz

2006

Eur J Inorg Chem

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“…Their potential has already been demonstrated in drug screening assays, 1 luminescent cellular imaging, 2 homogenous 3 and heterogeneous 4 immunoassays, FRET fluoroassays 5 or in bioassays to determine protein concentration. 6 Typically, an LLC contains an organic chelate, central lanthanide ion, and optionally, a molecular spacer terminated with a functional group.…”

Section: Introductionmentioning

confidence: 99%

Photophysical evaluation of a new functional terbium complex in FRET-based time-resolved homogenous fluoroassays

Cywiński

Nono²,

Charbonnière³

et al. 2014

Phys. Chem. Chem. Phys.

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A new functional luminescent lanthanide complex (LLC) has been synthesized with terbium as a central lanthanide ion and biotin as a functional moiety. Unlike in typical lanthanide complexes assembled via carboxylic moieties, in the presented complex, four phosphate groups are chelating the central lanthanide ion. This special chemical assembly enhances the complex stability in phosphate buffers conventionally used in biochemistry. The complex synthesis strategy and photophysical properties are described as well as the performance in time-resolved Förster Resonance Energy Transfer (FRET) assays. In those assays, this biotin-LLC transferred energy either to acceptor organic dyes (Cy5 or AF680) labelled on streptavidin or to quantum dots (QD655 or QD705) surfacefunctionalised with streptavidins. The permanent spatial donor-acceptor proximity is assured through strong and stable biotin-streptavidin binding. The energy transfer is evidenced from the quenching observed in donor emission and from a decrease in donor luminescence decay, both associated with simultaneous increase in acceptor intensity and in the decay time. The dye-based assays are realised in TRIS and in PBS, whereas QD-based systems are studied in borate buffer. The delayed emission analysis allows for quantifying the recognition process and for auto-fluorescence-free detection, which is particularly relevant for application in bioanalysis. In accordance with Förster theory, Förster-radii (R 0 ) were found to be around 60 Å for organic dyes and around 105 Å for QDs. The FRET efficiency (Z) reached 80% and 25% for dye and QD acceptors, respectively. Physical donor-acceptor distances (r) have been determined in the range 45-60 Å for organic dye acceptors, while for acceptor QDs between 120 Å and 145 Å. This newly synthesised biotin-LLC extends the class of highly sensitive analytical tools to be applied in the bioanalytical methods such as time-resolved fluoroimmunoassays (TR-FIA), luminescent imaging and biosensing.

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“…As a result, kinetic assays have been developed based on spectrophotometry/reflectometry, [28,29] fluorimetry, [30,31] chemiluminescence, [32][33][34] electrochemiluminescence, [35] and electroanalysis. [36,37] Among the fluorimetric methods, those based on time-resolution [38] (often referred to as time-resolved or gated assays) are the most sensitive tools in biological assays. Surprisingly, there has been only one report [39] on the application of time-resolved lanthanide luminescence (using Tb 3 + ), although it is a particularly attractive scheme for the determination of the activity of POx.…”

mentioning

confidence: 99%

A Novel Method for Time‐Resolved Fluorimetric Determination and Imaging of the Activity of Peroxidase, and Its Application to an Enzyme‐Linked Immunosorbent Assay

Lin

Wolfbeis

et al. 2006

Chemistry A European J

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A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H(2)O(2) (HP), using a fluorescent europium-tetracycline (Eu(3)TC) complex as indicator. On addition of HP, this complex forms a highly fluorescent adduct (Eu(3)TC-HP), which is decomposed in the presence of POx to form the weakly fluorescent europium-tetracycline (Eu(3)TC). Hence, the activity of the enzyme can be directly determined by means of the luminescent Eu(3)TC complex as indicator. The POx assay demonstrated herein was elaborated starting from a spectral characterization of the complex systems involved. Due to the long lifetime of lanthanide luminescence, both steady-state and time-resolved luminescence assays can easily be performed. The time-resolved assay can quantify POx in the range from 4.0 x 10(-5) to 5.9 x 10(-3) U mL(-1), with a limit of detection of 1.0 x 10(-5) U mL(-1). The effects of POx inhibitors such as cyanide, hydroxylamine, and azide have also been studied. In addition, a time-resolved fluorescent detection method for a POx-based enzyme-linked immunosorbent assay (ELISA) has been developed, which is demonstrated in a sandwich model assay with bovine IgG serving as analyte. Furthermore, a time-resolved fluorescent imaging method is demonstrated that makes use of a straightforward imaging set-up adjusted to the optical properties of the europium reagent.

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Time-resolved detection of lanthanide luminescence for ultrasensitive bioanalytical assays

Cited by 169 publications

References 97 publications

Ten‐Coordinate Neodymium(III) Complexes with Triethylenetetraaminehexaacetic Acid

Ten‐Coordinate Neodymium(III) Complexes with Triethylenetetraaminehexaacetic Acid

Photophysical evaluation of a new functional terbium complex in FRET-based time-resolved homogenous fluoroassays

A Novel Method for Time‐Resolved Fluorimetric Determination and Imaging of the Activity of Peroxidase, and Its Application to an Enzyme‐Linked Immunosorbent Assay

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