Time-resolved botulinum neurotoxin A activity monitored using peptide-functionalized Au nanoparticle energy transfer sensors

Wang, Yi; Liu, Xiaohu; Zhang, Jinling; Aili, Daniel; Liedberg, Bo

doi:10.1039/c3sc53305k

Cited by 32 publications

(20 citation statements)

References 61 publications

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“…One of these such systems is surface plasmon resonance, this has seen development from initial uses [121,122] to more recent and emerging advancements [81,123]. Other such techniques that were first explored several years ago are time-resolved fluorescence assay [124] and liquid chromatography [125]; these have more recently been explored, providing a relatively low level of detection and short detection time [40,126]. The problem with these techniques as detection platforms, is that they often require expensive instrumentation or have simply not been tested across a wide enough set of parameters.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Botulinum Neurotoxins—A Review

Hobbs

Thomas

Halliwell

et al. 2019

Toxins

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A toxin is a poisonous substance produced within living cells or organisms. One of the most potent groups of toxins currently known are the Botulinum Neurotoxins (BoNTs). These are so deadly that as little as 62 ng could kill an average human; to put this into context that is approximately 200,000 × less than the weight of a grain of sand. The extreme toxicity of BoNTs leads to the need for methods of determining their concentration at very low levels of sensitivity. Currently the mouse bioassay is the most widely used detection method monitoring the activity of the toxin; however, this assay is not only lengthy, it also has both cost and ethical issues due to the use of live animals. This review focuses on detection methods both existing and emerging that remove the need for the use of animals and will look at three areas; speed of detection, sensitivity of detection and finally cost. The assays will have wide reaching interest, ranging from the pharmaceutical/clinical industry for production quality management or as a point of care sensor in suspected cases of botulism, the food industry as a quality control measure, to the military, detecting BoNT that has been potentially used as a bio warfare agent.

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Section: Discussionmentioning

confidence: 99%

Rapid Detection of Botulinum Neurotoxins—A Review

Hobbs

Thomas

Halliwell

et al. 2019

Toxins

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“…One of the several concerns that must be solved in this case was the issue of steric hindrance that could turn peptide substrates inaccessible to proteases leading to a potential failure of hydrolysis. [ 71,72 ]…”

Section: Targeting Molecules Via Peptide–gold Hybrid Nanomaterialsmentioning

confidence: 99%

“…Following this strategy, Wang and his colleagues utilized designed peptide substrate and small mono‐maleimide AuNP conjugates as the linker and quencher. [ 71 ] With the help of fluorescence‐labeled streptavidin, various concentrations of BoLcA and trypsin were probed in a time‐dependent manner (Figure 7B,C). The LOD of BoLcA detection was estimated as low as 1 p m (Figure 7D).…”

Section: Targeting Molecules Via Peptide–gold Hybrid Nanomaterialsmentioning

confidence: 99%

Rational Design of Functional Peptide–Gold Hybrid Nanomaterials for Molecular Interactions

et al. 2020

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Gold nanoparticles (AuNPs) have been extensively used for decades in biosensing‐related development due to outstanding optical properties. Peptides, as newly realized functional biomolecules, are promising candidates of replacing antibodies, receptors, and substrates for specific molecular interactions. Both peptides and AuNPs are robust and easily synthesized at relatively low cost. Hence, peptide–AuNP‐based bio‐nano‐technological approaches have drawn increasing interest, especially in the field of molecular targeting, cell imaging, drug delivery, and therapy. Many excellent works in these areas have been reported: demonstrating novel ideas, exploring new targets, and facilitating advanced diagnostic and therapeutic technologies. Importantly, some of them also have been employed to address real practical problems, especially in remote and less privileged areas. This contribution focuses on the application of peptide–gold hybrid nanomaterials for various molecular interactions, especially in biosensing/diagnostics and cell targeting/imaging, as well as for the development of highly active antimicrobial/antifouling coating strategies. Rationally designed peptide–gold nanomaterials with functional properties are discussed along with future challenges and opportunities.

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“…Colorimetric, fluorimetric and luminescence methods were developed [91][92][93][94], however these did not show a suitable limit of detection or if they did, the need of specialized equipment, make them unsuitable for point-of-care applications. Shan Chen et.…”

mentioning

confidence: 99%