Time-resolved absorption as optical method for herbicide detection

Baldini, Francesco; Domenici, Claudio; Giannetti, Ambra; Masci, Domenica; Mencaglia, A. A.

doi:10.1016/s0925-4005(03)00028-5

Cited by 12 publications

(7 citation statements)

References 16 publications

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“…20 In addition, the sensitivity of RCs to certain herbicides, such as triazines (which block electron transfer by displacement of the secondary quinone), has been used to develop herbicide sensors based on inhibition of the measured photocurrent. 21,22 A major issue in many of the previous studies has been the connection between the reaction center and the electrode. 31 Lebedev et al (2006) demonstrated that cytochrome c could be employed as a redox wire, reducing the electron tunneling distance to P and thus improving the connection between P and the electrode surface.…”

Section: ■ Introductionmentioning

confidence: 99%

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Photocurrent Generation by Photosynthetic Purple Bacterial Reaction Centers Interfaced with a Porous Antimony-Doped Tin Oxide (ATO) Electrode

Carey

Zhang

Mieritz

et al. 2016

ACS Appl. Mater. Interfaces

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The ability to exchange energy and information between biological and electronic materials is critical in the development of hybrid electronic systems in biomedicine, environmental sensing, and energy applications. While sensor technology has been extensively developed to collect detailed molecular information, less work has been done on systems that can specifically modulate the chemistry of the environment with temporal and spatial control. The bacterial photosynthetic reaction center represents an ideal photonic component of such a system in that it is capable of modifying local chemistry via light-driven redox reactions with quantitative control over reaction rates and has inherent spectroscopic probes for monitoring function. Here a well-characterized model system is presented, consisting of a transparent, porous electrode (antimony-doped tin oxide) which is electrochemically coupled to the reaction center via a cytochrome c molecule. Upon illumination, the reaction center performs the 2-step, 2-electron reduction of a ubiquinone derivative which exchanges with oxidized quinone in solution. Electrons from the electrode then move through the cytochrome to reoxidize the reaction center electron donor. The result is a facile platform for performing redox chemistry that can be optically and electronically controlled in time and space.

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Section: ■ Introductionmentioning

confidence: 99%

“…There have been a number of previous reports of bacterial RCs interfaced with electrodes for use in integrated optical electronic devices, photovoltaics, and biosensors (e.g., refs − ). In the field of molecular electronics, for example, RCs isolated from Rb.…”

Section: Introductionmentioning

confidence: 99%

Photocurrent Generation by Photosynthetic Purple Bacterial Reaction Centers Interfaced with a Porous Antimony-Doped Tin Oxide (ATO) Electrode

Carey

Zhang

Mieritz

et al. 2016

ACS Appl. Mater. Interfaces

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“…Several research groups have used this to study binding of herbicides or other inhibitors by purified Rba. sphaeroides reaction centres in solution ( Jockers et al, 1993; Spyridaki et al, 2000; Baldini et al, 2003; Andreu et al, 2005 ), reconstituted into liposomes ( Peters et al, 1997 ), or embedded in a cationic polymer ( Mallardi et al, 2007; Giustini et al, 2012 ). Data analysis in most of these studies required the application of a mathematical model to translate changes in the kinetics of P photooxidation or subsequent recovery into the amount of herbicide binding at the Q B site (e.g.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a biohybrid photoelectrochemical cell employing the purple bacterial reaction centre as a biosensor for herbicides

Swainsbury

Friebe

Frese

et al. 2014

Biosensors and Bioelectronics

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The Rhodobacter sphaeroides reaction centre is a relatively robust and tractable membrane protein that has potential for exploitation in technological applications, including biohybrid devices for photovoltaics and biosensing. This report assessed the usefulness of the photocurrent generated by this reaction centre adhered to a small working electrode as the basis for a biosensor for classes of herbicides used extensively for the control of weeds in major agricultural crops. Photocurrent generation was inhibited in a concentration-dependent manner by the triazides atrazine and terbutryn, but not by nitrile or phenylurea herbicides. Measurements of the effects of these herbicides on the kinetics of charge recombination in photo-oxidised reaction centres in solution showed the same selectivity of response. Titrations of reaction centre photocurrents yielded half maximal inhibitory concentrations of 208 nM and 2.1 µM for terbutryn and atrazine, respectively, with limits of detection estimated at around 8 nM and 50 nM, respectively. Photocurrent attenuation provided a direct measure of herbicide concentration, with no need for model-dependent kinetic analysis of the signal used for detection or the use of prohibitively complex instrumentation, and prospects for the use of protein engineering to develop the sensitivity and selectivity of herbicide binding by the Rba. sphaeroides reaction centre are discussed.

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“…In general, biosensors consist of two components: a biomolecule, which is a highly specific recognition element, and a transducer, such as an electrode [3], or an optical fiber [4], that converts the molecular recognition event into a quantifiable signal. Signal transduction has been carried out with electrochemical [5], quartz crystal microbalance (QCM) [6], optical absorption [7], fluorescence [8], surface plasmon resonance (SPR) [9], and other transducers. In these biosensors, biomolecules (ligand), such as enzymes [10], antibodies [11], oligonucleotides [12][13][14], microorganisms [15], peptides [16], cells [17] were immobilized on a solid substrate by numerous steps and used to detect the presence of an analyte, such as enzymatic substrates, antigens, oligonucleotides and so on.…”

Section: Introductionmentioning

confidence: 99%

Localized surface plasmon resonance based optical biosensor using surface modified nanoparticle layer for label-free monitoring of antigen–antibody reaction

Endo

Yamamura

Nagatani

et al. 2005

Science and Technology of Advanced Materials

124

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In recent years, label-free biosensors not requiring external modifications have been receiving intense attention. A label-free optical biosensor, which retains many of the desirable features of conventional surface plasmon resonance (SPR) reflectometry, namely, the ability to monitor the kinetics of biomolecular interactions in real-time without a label has been developed with several important advantages: the biosensor device is easy to fabricate, and simple to implement, requiring only an UV-Vis spectrophotometer or flatbed scanner. Importantly, the label-free optical biosensor can be easily multiplexed to enable high-throughput monitoring of biomolecular interactions in an arraybased format. In this research, the development of a localized surface plasmon resonance (LSPR)-based label-free optical biosensor using a surface modified nanoparticle layer is aimed. This optical detection method promises to offer a massively parallel detection capability in a highly miniaturized package. The two-dimensional nanoparticle layer was formed by the surface modified silica nanoparticles. The optical properties and surface analysis of nanoparticle layer substrate were characterized through transmission measurements and atomic force microscopy (AFM). Simultaneously, the nanoparticle layer substrate was applied to the optical LSPR-based biosensor for label-free monitoring of the antigen-antibody reaction. The anti-fibrinogen antibody was immobilized onto the nanoparticle layer substrate surface. Different concentrations of fibrinogen were introduced to the anti-fibrinogen antibody immobilized nanoparticle layer substrate surface, and the change in the absorption spectrum, caused by the antigen-antibody reaction, was observed. By using this anti-fibrinogen antibody immobilized nanoparticle layer substrate; the detection limit of this optical LSPR-based biosensor was 10 ng/ml.

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Time-resolved absorption as optical method for herbicide detection

Cited by 12 publications

References 16 publications

Photocurrent Generation by Photosynthetic Purple Bacterial Reaction Centers Interfaced with a Porous Antimony-Doped Tin Oxide (ATO) Electrode

Photocurrent Generation by Photosynthetic Purple Bacterial Reaction Centers Interfaced with a Porous Antimony-Doped Tin Oxide (ATO) Electrode

Evaluation of a biohybrid photoelectrochemical cell employing the purple bacterial reaction centre as a biosensor for herbicides

Localized surface plasmon resonance based optical biosensor using surface modified nanoparticle layer for label-free monitoring of antigen–antibody reaction

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