Time-related bisphenol-A content and estrogenic activity in saliva samples collected in relation to placement of fissure sealants

Abstract

It was recently reported that estrogenic activity was detected in saliva samples collected during 1 h after placement of one fissure sealant (Delton) and this related to Bisphenol-A (BPA) content. The aim of the present study was to determine the time-related BPA content and estrogenic activity in saliva samples collected before and after placement of two fissure sealants each with a different monomer composition. Eight healthy male volunteers with no history of prior placement of fissure sealants or composite resin fillings had four molars sealed with either Delton LC (four people) or Visio-Seal (four people). Base-line saliva samples were collected preexperimentally, in the morning when fasting. Fissure sealants were placed and saliva samples collected immediately, 1 h and 24 hs after placement of the fissure sealant. BPA was found in saliva samples collected immediately after placement of Delton LC (range 0.3-2.8 ppm). No detectable amounts of BPA were determined 1 h and 24 h after Delton treatment (detection limit < or = 0.1 ppm). In base-line samples and in all samples collected from Visio-Seal treated individuals, no BPA was detected. In a recombinant yeast cell assay, significantly increased estrogenic activity was found in saliva samples collected immediately after placement of Delton LC sealant (P < 0.05; ANOVA) whereas no statistically significant estrogenic activity was observed in the remaining groups. In conclusion, minute amounts of BPA, however considerably lower than previously reported, were detected in saliva samples collected immediately after but not 1 and 24 h(s) after placement of Delton LC fissure sealant. BPA was not detected after placement of Visio-Seal fissure sealant.

BPA from dental resin material: where are we going with restorative and preventive dental biomaterials?

“…BPA was found in the saliva within few minutes after a dental treatment, but at considerably lower levels than previously reported. BPA became undetectable 1 and 24 h after the placement of the sealant [10,12]. It was concluded that due to the low level of BPA, there was no clinical risk after placement of fissure sealants, an assumption that was confirmed by other authors [10,13,14].…”

“…Again, these data depend upon the sensitivity of the analytical method. However, it could be clarified that Bis-DMA containing resins rather than Bis-GMA-based resins released BPA shortly after placement and that collected saliva showed estrogenic activity in vitro [10][11][12]. Under hydrolytic conditions, the release of BPA from a fissure sealant into the saliva occurs immediately after the placement of the sealant.…”

Infant Exposure to Bisphenol A Can Be Quantitatively Assessed by a Simply Improved High-Performance Liquid Chromatography–Tandem Mass-Spectrometry Method

“…[1][2][3] With a worldwide annual production estimated to be more than 3.5 million tons, BPA has shown endocrine-disrupting effects and potential adverse health effects. 4,5 These may cause severe environmental health problems and have thus triggered the increasing public concerns.…”

Comments on "Estrogenicity of Resin-Based Composites and Sealants Used in Dentistry"

“…I am concerned about continued reference to this study. Ashby (2) (3)(4)(5). On the other hand, BPDMA is a component of the sealant used in their study.…”

“…On the other hand, BPDMA is a component of the sealant used in their study. Therefore, leaching of a small amount of unpolymerized BPDMA is likely (3,4). Furthermore, the detection of BPA derived from BPDMA in saliva is likely because BPDMA is easily hydrolyzed to BPA in saliva (6) Olea's Response…”