Time of Flight Secondary Ion Mass Spectrometry imaging for precise localization of zirconium-labelled trastuzumab in xenograft cancer tumour tissues

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…The results revealed accumulation of iron in mitochondria, lysosomes and vacuole-like organelles, with SIMS providing more in-depth chemical data, providing insights into Fe metabolism at the cellular level. Penen et al 187 utilised TOF-SIMS imaging to investigate the distribution of the cancer drug trastuzumab which is used for tumours with high levels of human epidermal growth factor receptor 2 (HER2) present, such as ovarian and breast cancers. Mouse models were exposed to Zr-labelled trastuzumab and sacrificed 3 days after exposure, with tumours extracted, frozen at −80 °C and sectioned.…”