Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli

Hasperué, Joaquín H.; Gómez-Lobato, María E.; Chaves, Alicia Raquel; Civello, Pedro Marcos; Martínez, Gustavo

doi:10.1016/j.postharvbio.2013.02.021

Cited by 31 publications

(29 citation statements)

References 40 publications

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“…Chlorophyll content was measured following the method reported by Hasperué, Gómez‐Lobato, Chaves, Civello, and Martínez (). Briefly, three replicate samples of 0.5‐g frozen tissue were homogenized in 25 ml of 80% (v/v) acetone (AR, Beijing Chemical Factory, China).…”

Section: Methodsmentioning

confidence: 99%

Effect of ozone on the activity of antioxidant and chlorophyll‐degrading enzymes during postharvest storage of coriander ( Coriandrum sativum L.)

Shi

Jia

et al. 2019

J Food Process Preserv

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Coriander is vulnerable to a loss in quality during storage. To improve postharvest quality, 0.68 mg/L ozone (O3) was used to treat coriander prior to storage at 20 ± 1°C. Results indicated that O3 maintained the physical appearance of the harvested coriander and suppressed both the respiration rate and polyphenol oxidase activity. O3 treatment inhibited the decline in chlorophyll content and inhibited the activity of the chlorophyll‐degrading enzymes: chlorophyllase, chlorophyll‐degrading peroxidase, Mg‐dechelatase, and pheophytinase. Results also indicated that O3 effectively inhibited the accumulation of hydrogen peroxide and the generation of superoxide anion while increasing the enzyme activity of peroxidase, catalase, and ascorbate peroxidase, compared to untreated coriander. Thus, treatment of coriander with ozone may be a useful approach for maintaining the quality and extending the shelf life of coriander through its regulation of antioxidant and chlorophyll‐degrading enzyme activity. Practical applications Coriander is prone to postharvest yellowing or wilting of its leaves when stored at ambient temperature, and its quality deteriorates due to a rapid increase in senescence. Ozone treatments have been reported to induce disease resistance, stimulate the response to oxidative stress in plants, and effectively delay the decay of fruits and vegetables. Coriander treated with ozone may be a useful approach for maintaining postharvest quality through its regulation of antioxidant and chlorophyll‐degrading enzyme activity. The study demonstrated that ozone is a potential method for suppressing the postharvest quality deterioration in horticultural corps.

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Section: Methodsmentioning

confidence: 99%

Effect of ozone on the activity of antioxidant and chlorophyll‐degrading enzymes during postharvest storage of coriander ( Coriandrum sativum L.)

Shi

Jia

et al. 2019

J Food Process Preserv

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“…The resulting cDNA samples were diluted to 1/10 their concentrations (v/v) for qRT-PCR. Previously reported primer sets of GS biosynthesis ( BoCYP79B2 , BoCYP83B1 , BoCYP79F1 , and BoCYP83A1 ) genes, hydrolysis ( BoMyo , BoESP , and BoESM1 ) genes, a pathogenesis-related (PR) protein ( BoPR ) gene known to be responsive to MeJA, chlorophyll catabolism ( BoPPH and BoPaO ) genes, and the broccoli actin gene ( BoACT1 ) as a normalization standard were used for qRT-PCR [41,42] (Table 1). Transcript abundance of the broccoli actin ( BoACT1 ) gene from each treatment was stable throughout post-harvest storage (Figure S2).…”

Section: Methodsmentioning

confidence: 99%

Methyl Jasmonate and 1-Methylcyclopropene Treatment Effects on Quinone Reductase Inducing Activity and Post-Harvest Quality of Broccoli

Choi

Kim

et al. 2013

PLoS ONE

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Effect of pre-harvest methyl jasmonate (MeJA) and post-harvest 1-methylcyclopropene (1-MCP) treatments on broccoli floret glucosinolate (GS) concentrations and quinone reductase (QR, an in vitro anti-cancer biomarker) inducing activity were evaluated two days prior to harvest, at harvest and at 10, 20, and 30 days of post-harvest storage at 4 °C. MeJA treatments four days prior to harvest of broccoli heads was observed to significantly increase floret ethylene biosynthesis resulting in chlorophyll catabolism during post-harvest storage and reduced product quality. Post-harvest treatment with 1-methylcyclopropene (1-MCP), which competitively binds to protein ethylene receptors, maintained post-harvest floret chlorophyll concentrations and product visual quality in both control and MeJA-treated broccoli. Transcript abundance of BoPPH, a gene which is responsible for the synthesis of pheophytinase, the primary enzyme associated with chlorophyll catabolism in broccoli, was reduced by 1-MCP treatment and showed a significant, negative correlation with floret chlorophyll concentrations. The GS, glucobrassicin, neoglucobrassicin, and gluconasturtiin were significantly increased by MeJA treatments. The products of some of the GS from endogenous myrosinase hydrolysis [sulforaphane (SF), neoascorbigen (NeoASG), N-methoxyindole-3-carbinol (NI3C), and phenethyl isothiocyanate (PEITC)] were also quantified and found to be significantly correlated with QR. Sulforaphane, the isothiocyanate hydrolysis product of the GS glucoraphanin, was found to be the most potent QR induction agent. Increased sulforaphane formation from the hydrolysis of glucoraphanin was associated with up-regulated gene expression of myrosinase (BoMyo) and the myrosinase enzyme co-factor gene, epithiospecifier modifier1 (BoESM1). This study demonstrates the combined treatment of MeJA and 1-MCP increased QR activity without post-harvest quality loss.

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“…The quality and postharvest shelf life of baby spinach can be affected by a range of pre-harvest factors such as genotype, maturity stage, growing conditions and proper harvesting time (Howard et al, 2002;Clarkson et al, 2005;Pandjaitan et al, 2005;Conte et al, 2008;Chang et al, 2013). In particular, the time of day for harvest has been reported to influence quality and the composition of bioactive compounds of fresh products (Khalid et al, 2009;Moghaddam et al, 2013;Hasperué et al, 2013Hasperué et al, , 2014. In baby leaves, significant improvements to postharvest shelf life have been described as being achieved by rescheduling the time of day for harvest to end-of-day harvest (Clarkson et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…However, specific reports for vegetables have described that time of day for harvest has a variable response depending on the crop. In broccoli, changes in the metabolism due to time of day for harvest influenced the expression of genes during the day and also caused different patterns of expression during postharvest (Hasperué et al, 2013). Harvesting at the end of day maintained the green color of the inflorescence and the sugar levels of broccoli during postharvest (Hasperué et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Time of day for harvest and delay before processing affect the quality of minimally processed baby spinach

Garrido

Tudela

Gil

2015

Postharvest Biology and Technology

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Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli

Cited by 31 publications

References 40 publications

Effect of ozone on the activity of antioxidant and chlorophyll‐degrading enzymes during postharvest storage of coriander ( Coriandrum sativum L.)

Effect of ozone on the activity of antioxidant and chlorophyll‐degrading enzymes during postharvest storage of coriander ( Coriandrum sativum L.)

Methyl Jasmonate and 1-Methylcyclopropene Treatment Effects on Quinone Reductase Inducing Activity and Post-Harvest Quality of Broccoli

Time of day for harvest and delay before processing affect the quality of minimally processed baby spinach

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