Time-Lapse Video Microscopy of Wound Recovery and Reproduction in the Siphonous Green Alga Derbesia tenuissima

Martin, Erika C.; Jeremy, P T Ward; Kim, Gwang Hoon; West, John A.

doi:10.4490/algae.2006.21.1.109

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…marine cyanobacterium were initially collected; however, the cyanobacterium died, leaving a fine siphonous green unialga alive in the culture flask, codified as ASF14JUL14-1A. Based on its microscopic morphology, this sample was identified as a Derbesia sp. ,,, No attempt was made to produce axenic cultures of this cultured Chlorophyte; however, there was no visible evidence of microbial growth. The cultures were initially grown in 12 × 250 mL autoclaved flasks with SWBG11 media under 27.0–27.6 °C and 16 h/8 h light/dark cycle at 400–650 l× condition with semicontinuous dilution. ,, After 2 weeks of growth, the cultures were transferred into autoclaved 2 L flasks for another one month until they fully occupied the culture volume.…”

Section: Methodsmentioning

confidence: 99%

“…Based on its microscopic morphology, this sample was identified as a Derbesia sp. 21,22,53,54 No attempt was made to produce axenic cultures of this cultured Chlorophyte; however, there was no visible evidence of microbial growth. The cultures were initially grown in 12 × 250 mL autoclaved flasks with SWBG11 media under 27.0−27.6 °C and 16 h/8 h light/dark cycle at 400−650 l× condition with semicontinuous dilution.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Pagoamide A, a Cyclic Depsipeptide Isolated from a Cultured Marine Chlorophyte, Derbesia sp., Using MS/MS-Based Molecular Networking

Zhang

et al. 2020

J. Nat. Prod.

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A thiazole-containing cyclic depsipeptide with 11 amino acid residues, named pagoamide A (1), was isolated from laboratory cultures of a marine Chlorophyte, Derbesia sp. This green algal sample was collected from America Samoa, and pagoamide A was isolated using guidance by MS/MSbased molecular networking. Cultures were grown in a lightand temperature-controlled environment and harvested after several months of growth. The planar structure of pagoamide A (1) was characterized by detailed 1D and 2D NMR experiments along with MS and UV analysis. The absolute configurations of its amino acid residues were determined by advanced Marfey's analysis following chemical hydrolysis and hydrazinolysis reactions. Two of the residues in pagoamide A (1), phenylalanine and serine, each occurred twice in the molecule, once in the D-and once in the L-configuration. The biosynthetic origin of pagoamide A (1) was considered in light of other natural products investigations with coenocytic green algae.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Pagoamide A, a Cyclic Depsipeptide Isolated from a Cultured Marine Chlorophyte, Derbesia sp., Using MS/MS-Based Molecular Networking

Zhang

et al. 2020

J. Nat. Prod.

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“…Some marine coenocytic green algae possess the unique ability to regenerate new functional cells from small droplets of protoplasm extruded in seawater (Pak et al 1991;Kim et al 2001Kim et al , 2002Kim et al , 2006Klotchkova et al 2003Klotchkova et al , 2005Kim and Klotchkova 2004;Martin et al 2006;Yoon et al 2008). In these algae, when protoplasm is leaking from the damaged cells, the organelles aggregate in vitro and form protoplasts, and a new cell membrane is generated around them.…”

Section: Introductionmentioning

confidence: 96%

Purification and characterization of a lectin, BPL-3, from the marine green alga Bryopsis plumosa

et al. 2010

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A novel lectin was isolated and characterized from Bryopsis plumosa (Hudson) Agardh and named BPL-3. This lectin showed specificity to N-acetyl-D-galactosamine as well as N-acetyl-D-glucosamine and agglutinated human erythrocytes of all blood types, showing slight preference to the type A. SDS-PAGE and MALDI-TOF MS data showed that BPL-3 was a monomeric protein with molecular weight of 11.5 kDa. BPL-3 was a non-glycoprotein with pI value of ∼7.0. It was stable in high temperatures up to 70°C and exhibited optimum activity in pH 5.5-10. The N-terminal and internal amino acid sequences of the lectin were determined by Edman degradation and enzymatic digestion, which showed no sequence homology to any other reported proteins. The full sequence of the cDNA encoding this lectin was obtained from PCR using cDNA library, and the degenerate primers were designed from the N-terminal amino acid sequence. The size of the cDNA was 622 bp containing single ORF encoding the lectin precursor. This lectin showed the same sugar specificity to previously reported lectin, Bryohealin, involved in protoplast regeneration of B. plumosa. However, the amino acid sequences of the two lectins were completely different. The homology analysis of the full cDNA sequence of BPL-3 showed that it might belong to H lectin group, which was originally isolated from Roman snails.

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“…In some cases, gametes are completely released from the gametangium within a short time after the onset of discharge (Mine et al . 1996; Martin et al . 2006).…”

Section: Effects Of Incubation Of Gametangia In Artificial Seawater (mentioning

confidence: 99%

Research note: Induction of gamete discharge by hypertonic treatment in the green algaBryopsis plumosa(Caulerpales, Chlorophyta)

Mine¹,

Ozaki

Sekida³

et al. 2010

Phycological Research

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Brief treatment with hypertonic solutions induced gamete discharge from gametangia in the coenocytic green alga Bryopsis plumosa (Hudson) C. Agardh. It is known that gamete discharge in this alga is triggered by a change from darkness to light. However, in this study mature gametangia, incubated for 30-120 s in artificial seawater (ASW) with an additional 0.4-0.6 M NaCl and then transferred into pure ASW, discharged gametes in darkness. The treatment did not affect the motility of the gametes. Addition of sucrose (1.0-1.2 M) to ASW also induced gamete discharge in darkness. Similar results were obtained by adding KCl (0.4 M) or mannitol (1.2 M) to ASW. Continuous incubation of gametangia in such hypertonic solutions also induced gamete discharge but led to a delay and a reduction in the rate of gamete discharge, and a loss of gamete motility. In gametangia treated with the hypertonic solutions, as well as in those exposed to light, shortening of the gametangial length was observed before gamete discharge.p re_601 70..73

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Time-Lapse Video Microscopy of Wound Recovery and Reproduction in the Siphonous Green Alga Derbesia tenuissima

Cited by 3 publications

References 24 publications

Pagoamide A, a Cyclic Depsipeptide Isolated from a Cultured Marine Chlorophyte, Derbesia sp., Using MS/MS-Based Molecular Networking

Pagoamide A, a Cyclic Depsipeptide Isolated from a Cultured Marine Chlorophyte, Derbesia sp., Using MS/MS-Based Molecular Networking

Purification and characterization of a lectin, BPL-3, from the marine green alga Bryopsis plumosa

Research note: Induction of gamete discharge by hypertonic treatment in the green algaBryopsis plumosa(Caulerpales, Chlorophyta)

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