Time‐lapse analysis of stem‐cell divisions in the Arabidopsis thaliana root meristem

Abstract

SummaryIn the Arabidopsis root, asymmetric stem-cell divisions produce daughters that form the different root cell types. Here we report the establishment of a confocal tracking system that allows the analysis of numbers and orientations of cell divisions in root stem cells. The system provides direct evidence that stem cells have lower division rates than cells in the proximal meristem. It also allows tracking of cell division timing, which we have used to analyse the synchronization of root cap divisions. Finally, it gives new insights into lateral root cap formation: epidermal stem-cell daughters can rotate the orientation of the division plane like the stem cell.

PaCeQuant: A Tool for High-Throughput Quantification of Pavement Cell Shape Characteristics

“…To study developmental shape transitions and molecular and genetic modes of shape establishment, imaging must be combined with the quantification of PC shape characteristics. A combination of advanced imaging, automatic quantification of shape features, and computational modeling has been used to study the development of embryos (De Rybel et al, 2014;Yoshida et al, 2014), of root meristems (Campilho et al, 2006), of shoots apical meristems (Reddy et al, 2004;Kierzkowski et al, 2012;Serrano-Mislata et al, 2015), and of flower organs . In these studies, the direction of cell division and cell expansion is tracked over time from semi-and fully automatic 3D segmentations of individual cells using the MorphoGraphX software Barbier de Reuille et al, 2015).…”

Jasmonate Controls Leaf Growth by Repressing Cell Proliferation and the Onset of Endoreduplication while Maintaining a Potential Stand-By Mode

“…The Arabidopsis (Arabidopsis thaliana) lines coi1-16 (At2g39940; , subjected to backcrossing to eliminate a pen2 mutation (Westphal et al, 2008), aos (At5g42650; N6149), H2B-YFP (Campilho et al, 2006), and pCYCB1;1:Dbox-GUS (Colón-Carmona et al, 1999) have been described. For clarity, we refer to the coi1-16 line used here as coi1-16B (for backcrossed).…”

“…Finally, quantification of nuclear surface area visualized by histone 2B-yellow fluorescent protein (H2B-YFP; Campilho et al, 2006) fusion proteins using confocal microscopy was performed according to Ishida et al (2010) in 6-d-old wild-type roots untreated or continuously treated with MeJA. Following exposure to MeJA, the average size of the nuclei in Arabidopsis root cells of the meristematic and elongation zones was reduced (Fig.…”

Identification and characterization of mRNAs of receptor-like kinases MhyGSO1 and MhyGSO2 in flowering parasitic plant Monotropa hypopitys

“…В апикальной корневой меристеме эпидермис на кончи-ке корня формируется потомством инициалей колумеллы корневого чехлика в проксимальной меристеме (Campilho et al, 2006), над которой находится покоящийся центр (ПЦ, или QC) -группа из нескольких редко делящихся стволовых клеток (Drisch, Stahl, 2015;Додуева и др., 2016). Поддержанию популяции стволовых клеток в ПЦ способ-ствует фактор транскрипции WUS-RELATED HOMEO-BOX 5 (WOX5) (Drisch, Stahl, 2015;Ощепкова и др., 2016).…”