Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures

Zaidi, Noorzaileen Eileena; Shazali, Nur Aima Hafiza; Leow, Adam Thean Chor; Osman, Mohamad; Ibrahim, Kamariah; Jaoi-Edward, Marilyn; Rahman, Nik Mohd Afizan Nik Abd

doi:10.3791/60281-v

Cited by 2 publications

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“…Approximately 50% of the macrophage population has been detected in solid tumours and has been shown to perform an important pro-tumoral role [ 6 , 7 ]. Macrophages are heterogeneous and have a wide range of plasticity and functionality, allowing them to differentiate between separate phenotypes known as M1 (anti-tumoral phenotype) and M2 (pro-tumoral phenotype) depending on microenvironmental stimuli [ 8 , 9 ]. These macrophages are predominantly found infiltrated and differentiated within the TME and are known as “tumour-associated macrophages” or TAMs at all stages of tumour growth [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Crosstalk between Fatty Acid Metabolism and Tumour-Associated Macrophages in Cancer Progression

Zaidi¹,

Shazali²,

Leow³

et al. 2022

BioMedicine

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Summary Over the last few decades, cancer has been regarded as an independent and self sustaining progression. The earliest hallmarks of cancer comprise of sustaining proliferative signalling, avoiding growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, and activating invasion and metastasis. Nonetheless, two emerging hallmarks are being described: aberrant metabolic pathways and evasion of immune destruction. Changes in tumour cell metabolism are not restricted to tumour cells alone; the products of the altered metabolism have a direct impact on the activity of immune cells inside the tumour microenvironment, particularly tumour-associated macrophages (TAMs). The complicated process of cancer growth is orchestrated by metabolic changes dictating the tight mutual connection between these cells. Here, we discuss approaches to exploit the interaction of cancer cells’ abnormal metabolic activity and TAMs. We also describe ways to exploit it by reprogramming fatty acid metabolism via TAMs.

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Section: Introductionmentioning

confidence: 99%

Crosstalk between Fatty Acid Metabolism and Tumour-Associated Macrophages in Cancer Progression

Zaidi¹,

Shazali²,

Leow³

et al. 2022

BioMedicine

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Interleukin-27 Disrupts the Crosstalk of Apoptotic Activities between 4T1 Breast Cancer Cells and M2 Macrophages

Yusof¹,

Fuadi²,

Hamid³

et al. 2022

MJMHS

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Introduction: Cytokine immunotherapy such as Interleukin-27 (IL-27) has been foreseen as a promising alternative anti-cancer treatment. Thus, this study aimed to investigate whether IL-27 gene therapy regulates crosstalk between breast cancer cells and macrophages in the sense of pro-apoptotic activities. Methods: This study has led to the development of recombinant pcDNA3.4-IL27. The recombinant pcDNA3.4-IL27 was transfected into 4T1 murine mammary carcinoma cells alone and co-culture of 4T1 with M2 macrophages. The successful expression of IL-27 in the cells were determine through the immunofluorescence staining and detection of CD206, M2 macrophages marker. Apoptotic effects of pcDNA3.4-IL27 were assessed through MTT assay, Annexin V flow cytometer analysis, and AO/PI dual staining. Results: Our findings shows that pcDNA3.4-IL27 has the ability to induce apoptosis in both of the cell group and performs better in the co-culture of 4T1 with M2 macrophages compared to 4T1 cells alone. PcDNA3.4-IL27 induced apoptosis through the altered cell morphology and reduction in the number of viable cells. Conclusion: These data demonstrate that pcDNA3.4-IL27 has the ability to induce apoptosis in both 4T1 cell alone and co-cultured 4T1 with M2 macrophages. Thus, could serve as a potential anti cancer candidate against breast cancer.

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Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures

Cited by 2 publications

References 0 publications

Crosstalk between Fatty Acid Metabolism and Tumour-Associated Macrophages in Cancer Progression

Crosstalk between Fatty Acid Metabolism and Tumour-Associated Macrophages in Cancer Progression

Interleukin-27 Disrupts the Crosstalk of Apoptotic Activities between 4T1 Breast Cancer Cells and M2 Macrophages

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