Time-kill kinetics and antibacterial activity of ethanolic extract of Allium sativum

Ngene, Anayochukwu Chibuike; Ohaegbu, Chinedu; Idu, Emmanuel; Odo, Ekene

doi:10.21608/mid.2023.175501.1417

Cited by 2 publications

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“…The culture medium containing the tested bacteria without the presence of an herbal substance was used as a control. Each procedure was performed in triplicate (three independent experiments) [ 65 , 66 ].…”

Section: Methodsmentioning

confidence: 99%

“…Oral pathogens, and especially those involved in periodontitis, are among the most resistant to the antibiotics. Various studies have indicated not only the increased percentage of resistant isolates [66][67][68][69], but also a trend over the years towards decreasing susceptibility profiles as in the study of Jepsen et al, (2021), with antibiotic non-susceptibilities observed in 37% of patients in 2008 and in 70% in 2015 [70].…”

Section: The Analysis Of the Antimicrobial Activity Of Plant Extractsmentioning

confidence: 99%

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The In Vitro Assessment of Antibacterial and Antioxidant Efficacy in Rosa damascena and Hypericum perforatum Extracts against Pathogenic Strains in the Interplay of Dental Caries, Oral Health, and Food Microbiota

Antoniadou,

Rozos,

Vaiou

et al. 2023

Microorganisms

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The rising demand for novel antibiotic agents prompts an investigation into natural resources, notably plant-derived compounds. In this study, various extracts (aqueous, ethanolic, aqueous-ethanolic, and enzymatic) of Rosa damascena and Hypericum perforatum were systematically evaluated against bacterial strains isolated from dental lesions (n = 6) and food sources (raw milk and broiler carcass, n = 2). Minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), antibiofilm activity, and time-kill kinetics were assessed across a range of extract concentrations, revealing a dose-responsive effect. Notably, some extracts exhibited superior antibacterial efficacy compared to standard clinical antibiotics, and the time-kill kinetics demonstrated a rapid elimination of bacterial loads within 24 h. The susceptibility pattern proved strain-specific, contingent upon the extract type, yet all tested pathogens exhibited sensitivity. The identified extracts, rich in phenolic and polyphenolic compounds, as well as other antioxidant properties, contributed to their remarkable antibiotic effects. This comprehensive investigation not only highlights the potential of Rosa damascena and Hypericum perforatum extracts as potent antibacterial agents against diverse bacterial strains including caries pathogens, but also underscores their rapid action and dose-dependent efficacy. The findings suggest a promising avenue for harnessing plant-derived compounds in the development of novel antimicrobial strategies against dental caries and other oral inflammations, bridging the gap between natural resources and antibiotic discovery.

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Section: Methodsmentioning

confidence: 99%

Section: The Analysis Of the Antimicrobial Activity Of Plant Extractsmentioning

confidence: 99%

The In Vitro Assessment of Antibacterial and Antioxidant Efficacy in Rosa damascena and Hypericum perforatum Extracts against Pathogenic Strains in the Interplay of Dental Caries, Oral Health, and Food Microbiota

Antoniadou,

Rozos,

Vaiou

et al. 2023

Microorganisms

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Evaluation of antibacterial activity of an experimental dental adhesive containing synthesized quaternary ammonium compound: in vitro study

El-Deeb,

Ismail,

Kenawy

et al. 2024

Discov Appl Sci

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Background Dental adhesives with immobilized antibacterial agents are formulated to combat bacterial invasion along the tooth-restoration interface. This study aims to evaluate the antibacterial effect of synthesized quaternary ammonium compound (QAC) incorporated into commercial dental adhesive. Methods QAC was synthesized from 2-(Dimethylamino) ethyl methacrylate and 1-Bromobutane and characterized using CHN (Carbon, Hydrogen, Nitrogen), FTIR (Fourier transform infrared) and H+NMR (Proton nuclear magnetic resonance) analyses. The synthesized QAC was assessed for its cytotoxicity and its antibacterial activity against S. mutans using disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), time-kill kinetics test, and TEM imaging. The QAC was added to the primer of a commercially available adhesive (OptiBond XTR) at two concentrations; 20 and 40 mg mL−1 representing the MIC and MBC, respectively. The antibacterial properties of the experimental adhesives, commercial antibacterial adhesive Clearfil SE Protect containing 12-methacryloyloxydodecylpyridinium bromide (MDPB), and commercial vehicle (OptiBond XTR) were compared using time-kill kinetics test. Statistical analysis by ANOVA followed by tukey post-hoc test (P < 0.05). Results Disc diffusion and time-kill kinetics tests showed potent antibacterial action of QAC, both in the unpolymerized and the cured forms. MIC and MBC were 20 and 40 mg mL−1 respectively. There was no statistically significant difference between experimental adhesives and Clearfil Protect with more than 99% reduction in bacterial count, while OptiBond XTR showed no bacterial killing up for up to 10 h. Conclusions The synthesized QAC added to a commercially available adhesive imparted antibacterial properties, thus providing an affordable adhesive system to the local market.

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Time-kill kinetics and antibacterial activity of ethanolic extract of Allium sativum

Cited by 2 publications

References 0 publications

The In Vitro Assessment of Antibacterial and Antioxidant Efficacy in Rosa damascena and Hypericum perforatum Extracts against Pathogenic Strains in the Interplay of Dental Caries, Oral Health, and Food Microbiota

The In Vitro Assessment of Antibacterial and Antioxidant Efficacy in Rosa damascena and Hypericum perforatum Extracts against Pathogenic Strains in the Interplay of Dental Caries, Oral Health, and Food Microbiota

Evaluation of antibacterial activity of an experimental dental adhesive containing synthesized quaternary ammonium compound: in vitro study

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