Time-dependent post mortem changes in the composition of intestinal bacteria using real-time quantitative PCR

Tuomisto, Sari; Karhunen, Pekka J.

doi:10.1186/1757-4749-5-35

Cited by 28 publications

(22 citation statements)

References 17 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tuomisto et al . 35 observed that the amount of bacterial DNA remained quite stable with time elapsed post-mortem, except for a substantial increase on day 5 post-mortem, during autopsies of rectum from male human. Those results indicated that the number of bacteria increased while the richness declined during the decomposition process.…”

Section: Discussionmentioning

confidence: 87%

Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing

Guo

Liao

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Decomposition is a complex process involving the interaction of both biotic and abiotic factors. Microbes play a critical role in the process of carrion decomposition. In this study, we analysed bacterial communities from live rats and rat remains decomposed under natural conditions, or excluding sarcosaphagous insect interference, in China using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 1,394,842 high-quality sequences and 1,938 singleton operational taxonomic units were obtained. Bacterial communities showed notable variation in relative abundance and became more similar to each other across body sites during the decomposition process. As decomposition progressed, Proteobacteria (mostly Gammaproteobacteria) became the predominant phylum in both the buccal cavity and rectum, while Firmicutes and Bacteroidetes in the mouth and rectum, respectively, gradually decreased. In particular, the arrival and oviposition of sarcosaphagous insects had no obvious influence on bacterial taxa composition, but accelerated the loss of biomass. In contrast to the rectum, the microbial community structure in the buccal cavity of live rats differed considerably from that of rats immediately after death. Although this research indicates that bacterial communities can be used as a “microbial clock” for the estimation of post-mortem interval, further work is required to better understand this concept.

show abstract

Section: Discussionmentioning

confidence: 87%

Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing

Guo

Liao

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The fold value in target bacteria was calculated for each sample using the

2^{- normalΔ normalΔ C_{t}}

method (Tuomisto et al . ).…”

Section: Methodsmentioning

confidence: 97%

“…The threshold PCR cycle number (C t ) values were determined using the LIGHTCYCLER RELATIVE QUANTIFICATION software (Roche Applied Science, Mannheim, Germany). The fold value in target bacteria was calculated for each sample using the 2 ÀDDCt method (Tuomisto et al 2013).…”

Section: Faecal Microbiota Analysis By Real-time Pcrmentioning

confidence: 99%

Oral administration ofLactobacillus caseivarietyrhamnosuspartially alleviates TMA-induced atopic dermatitis in mice through improving intestinal microbiota

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Microorganisms exist both inside and outside the dead body and exhibit a distinct and temporal shift during the process of decay [22]. The gut, as an organ contains a large number of bacteria, and some bacteria have been shown to exhibit variations during the decomposition of corpses [11,23,24], which prompted this study. The bacterial communities in the decomposing rats showed a distinct shift in the categories and relative abundance compared with those in the living rats.…”

Section: Discussionmentioning

confidence: 99%

Potential Using of Molecular Characterization and Structure of The Gut Bacterial Community for Postmortem Interval Estimation in Sprague Dawley Rats

Zhang

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Once the body dies, the inherent microbes of the host begin to break down from the inside and play a key role thereafter. It is hypothesized that after the death certain rectal microbes would change during the decomposition course in the body. This study aimed to investigate the probable shift in the composition of the rectal flora at different time intervals up to 15 days after death and to explore bacterial taxa important for estimating the time of death. At the phylum level, Proteobacteria and Firmicutes showed major shifts, when checked at 11 different intervals, and emerged at most of the postmortem intervals. At the species level, Enterococcus faecalis and Proteus mirabilis existed at most postmortem intervals; the former showed a downward trend after day 5 postmortem, while the latter showed an upward trend. There were obvious differences in bacterial community structure and richness at the phylum, genus, and species levels during the decomposition of the corpse of rats. The phylum, genus, and species taxa richness decreased initially and then increased significantly. The turning point came on day 9 when genus, rather than phylum or species, contained the most information for estimating the time of death. We constructed a prediction model using genus taxon data from high-throughput sequencing, which explained 87.2% of the time since the first sampling within 1 h. Seven bacteria, namely Enterococcus, Proteus, Lactobacillus, unidentified Clostridiales, Vagococcus, unidentified Corynebacteriaceae, and unidentified Enterobacteriaceae, were included in this model. The above-mentioned bacteria showed a promising future for estimating the shortest time of death and results of current study were agreeing with the proposed hypothesis.

show abstract

Time-dependent post mortem changes in the composition of intestinal bacteria using real-time quantitative PCR

Cited by 28 publications

References 17 publications

Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing

Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing

Oral administration ofLactobacillus caseivarietyrhamnosuspartially alleviates TMA-induced atopic dermatitis in mice through improving intestinal microbiota

Potential Using of Molecular Characterization and Structure of The Gut Bacterial Community for Postmortem Interval Estimation in Sprague Dawley Rats

Contact Info

Product

Resources

About