Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography

Zinger, O.; Anselme, Karine; Denzer, Alain J.; Habersetzer, P; Wieland, Marco; Jeanfils, J.; Hardouin, Pierre; Landolt, D.

doi:10.1016/j.biomaterials.2003.09.111

Cited by 298 publications

(213 citation statements)

References 43 publications

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“…On the other hand, β-Ca 2 SiO 4 scaffolds had rough surface and pores on the struts, which might be the gas release that induced from the reaction during sintering process [2,3]. In fact, the rough surface is benefit for cell adhesion, differentiation and thereby formation of cell matrix [45–47]. Therefore, the 3D-printed preceramic polymer-derived scaffolds had a desirable phase of β-Ca 2 SiO 4 and rough surface, which might be promising for bone tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

3D printed porous β-Ca₂SiO₄scaffolds derived from preceramic resin and their physicochemical and biological properties

Liu

et al. 2018

Science and Technology of Advanced Materials

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Silicate bioceramic scaffolds are of great interest in bone tissue engineering, but the fabrication of silicate bioceramic scaffolds with complex geometries is still challenging. In this study, three-dimensional (3D) porous β-Ca2SiO4 scaffolds have been successfully fabricated from preceramic resin loaded with CaCO3 active filler by 3D printing. The fabricated β-Ca2SiO4 scaffolds had uniform interconnected macropores (ca. 400 μm), high porosity (>78%), enhanced mechanical strength (ca. 5.2 MPa), and excellent apatite mineralization ability. Importantly, the results showed that the increase of sintering temperature significantly enhanced the compressive strength and the scaffolds sintered at higher sintering temperature stimulated the adhesion, proliferation, alkaline phosphatase activity, and osteogenic-related gene expression of rat bone mesenchymal stem cells. Therefore, the 3D printed β-Ca2SiO4 scaffolds derived from preceramic resin and CaCO3 active fillers would be promising candidates for bone tissue engineering.

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Section: Discussionmentioning

confidence: 99%

3D printed porous β-Ca₂SiO₄scaffolds derived from preceramic resin and their physicochemical and biological properties

Liu

et al. 2018

Science and Technology of Advanced Materials

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“…However, useful information can be gained from previous studies that investigated either the role of surface roughness or wettability. At relatively high roughness (submicrometer down to ~100 nm), it is clear that substrate topography influences cell response, the cells responding to roughness by a higher cell thickness and a delayed apparition of the focal contacts [5,35] . At the nanometer scale, Washburn et al [36] showed that the rate of proliferation on the smooth regions of a poly(Llactic acid) film (below 1 nm) is much greater than on the rough regions (around 12 nm).…”

Section: Relative Importance Of Film Stiffness Roughness and Wettabimentioning

confidence: 99%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

244

263

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Beside chemical properties and topographical features, mechanical properties of gels have been recently demonstrated to play an important role in various cellular processes, including cell attachment, proliferation, and differentiation. In this work, we used multilayer films made of poly (L-lysine)/Hyaluronan (PLL/HA) of controlled stiffness to investigate the effects of mechanical properties of thin films on skeletal muscle cells (C2C12 cells) differentiation. Prior to differentiation, cells need to adhere and proliferate in growth medium. Stiff films (E 0 > 320 kPa) promoted formation of focal adhesions and organization of the cytoskeleton as well as an enhanced proliferation, whereas soft films were not favorable for cell anchoring, spreading or proliferation. Then C2C12 cells were switched to a low serum containing medium to induce cell differentiation, which was also greatly dependent on film stiffness. Although myogenin and troponin T expressions were only moderately affected by film stiffness, the morphology of the myotubes exhibited striking stiffness-dependent differences. Soft films allowed differentiation only for few days and the myotubes were very short and thick. Cell clumping followed by aggregates detachment could be observed after ~2 to 4 days. On stiffer films, significantly more elongated and thinner myotubes were observed for up to ~ 2 weeks. Myotube striation was also observed but only for the stiffer films. These results demonstrate that film stiffness modulates deeply adhesion, proliferation and differentiation, each of these processes having its own stiffness requirement.

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“…In general, the biocompatibility of bone-replacing implant materials is closely related to osteoblast adhesion onto their surface (12)(13)(14). Osteoblast attachment, adhesion and spreading will influence the capacity of these cells to proliferate and to differentiate itself upon contact with the implant.…”

Section: Cellular Interactions With Implant Surfacesmentioning

confidence: 99%

Organic–Inorganic Surface Modifications for Titanium Implant Surfaces

et al. 2008

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Abstract. This paper reviews current physicochemical and biochemical coating techniques that are investigated to enhance bone regeneration at the interface of titanium implant materials. By applying coatings onto titanium surfaces that mimic the organic and inorganic components of living bone tissue, a physiological transition between the non-physiological titanium surface and surrounding bone tissue can be established. In this way, the coated titanium implants stimulate bone formation from the implant surface, thereby enhancing early and strong fixation of bone-substituting implants. As such, a continuous transition from bone tissue to implant surface is induced. This review presents an overview of various techniques that can be used to this end, and that are inspired by either inorganic (calcium phosphate) or organic (extracellular matrix components, growth factors, enzymes, etc.) components of natural bone tissue. The combination, however, of both organic and inorganic constituents is expected to result into truly bone-resembling coatings, and as such to a new generation of surface-modified titanium implants with improved functionality and biological efficacy.

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Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography

Cited by 298 publications

References 43 publications

3D printed porous β-Ca₂SiO₄scaffolds derived from preceramic resin and their physicochemical and biological properties

3D printed porous β-Ca₂SiO₄scaffolds derived from preceramic resin and their physicochemical and biological properties

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Organic–Inorganic Surface Modifications for Titanium Implant Surfaces

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Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography

Cited by 298 publications

References 43 publications

3D printed porous β-Ca2SiO4scaffolds derived from preceramic resin and their physicochemical and biological properties

3D printed porous β-Ca2SiO4scaffolds derived from preceramic resin and their physicochemical and biological properties

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Organic–Inorganic Surface Modifications for Titanium Implant Surfaces

Contact Info

Product

Resources

About

3D printed porous β-Ca₂SiO₄scaffolds derived from preceramic resin and their physicochemical and biological properties

3D printed porous β-Ca₂SiO₄scaffolds derived from preceramic resin and their physicochemical and biological properties