Time-dependent microbial shifts during crayfish decomposition in freshwater and sediment under different environmental conditions

Mähler, Bastian; Janssen, Kathrin; Lönartz, Mara I.; Lagos, M.; Geisler, Thorsten; Rust, Jes; Bierbaum, Gabriele

doi:10.1038/s41598-023-28713-x

Cited by 7 publications

(10 citation statements)

References 96 publications

(106 reference statements)

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“…These facultatively anaerobic bacteria were observed in high abundance during the initial stages of the experiment in all plant biofilm samples. Domination of Aeromonas species during initial decay processes has been reported in previous studies about the decomposition of soft tissue materials 14 . In addition, filamentous bacteria of the genus Sphaerotilus and motile, microaerophilic species of Aquabacterium 25 , capable of accumulating large amounts of slime, were detected in high abundance during the first three weeks of study in the aerobic and anaerobic samples.…”

Section: Discussionsupporting

confidence: 66%

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Oxygen-dependent biofilm dynamics in leaf decay: an in vitro analysis

Karačić,

Palmer,

Gee

et al. 2024

Sci Rep

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Biofilms are important in the natural process of plant tissue degradation. However, fundamental knowledge of biofilm community structure and succession on decaying leaves under different oxygen conditions is limited. Here, we used 16S rRNA and ITS gene amplicon sequencing to investigate the composition, temporal dynamics, and community assembly processes of bacterial and fungal biofilms on decaying leaves in vitro. Leaves harvested from three plant species were immersed in lake water under aerobic and anaerobic conditions in vitro for three weeks. Biofilm-covered leaf samples were collected weekly and investigated by scanning electron microscopy. The results showed that community composition differed significantly between biofilm samples under aerobic and anaerobic conditions, though not among plant species. Over three weeks, a clear compositional shift of the bacterial and fungal biofilm communities was observed. The alpha diversity of prokaryotes increased over time in aerobic assays and decreased under anaerobic conditions. Oxygen availability and incubation time were found to be primary factors influencing the microbial diversity of biofilms on different decaying plant species in vitro. Null models suggest that stochastic processes governed the assembly of biofilm communities of decaying leaves in vitro in the early stages of biofilm formation and were further shaped by niche-associated factors.

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Section: Discussionsupporting

confidence: 66%

“…Sarkar et al (2022) emphasize that clostridia are effective H 2 and acid producers using carbohydrates such as cellulose and sucrose as substrates 34 . Numerous studies showed that saprophytic clostridia are associated with the decay of plants, phytoplankton, animal and human tissues 14 , 35 .…”

Section: Discussionmentioning

confidence: 99%

Oxygen-dependent biofilm dynamics in leaf decay: an in vitro analysis

Karačić,

Palmer,

Gee

et al. 2024

Sci Rep

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“…Among these, Nitrospira has good nitri cation ability, and Azospira can use nitrogen in sewage (Daims and Wagner 2018). Proteocatella is a facultative anaerobic bacterium that decomposes urea in sewage which is also involved in the fossilization process of shrimp carcasses in water (Mahler et al 2023), suggesting that it can reduce the pollutants produced by the decay of shrimp carcasses. Dechloromonas can realize simultaneous nitrogen and phosphorus removal in anoxic environments through denitri cation and phosphorus removal metabolism, further reducing COD and aeration energy consumption in the sewage treatment process (Zhao et al 2022).…”

Section: Discussionmentioning

confidence: 99%

A microbial flora with superior pollutant removal efficiency and its fermentation process optimization

Wang

et al. 2023

Preprint

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Microbial flora plays an important role in microorganism-enhanced technology. The pollutant degradation ability and viable counts of these agents are crucial to guarantee their practical application. In this study, an efficient pollutant-degrading microbial flora was screened, its medium components and culture conditions were optimized, and its effect was verified in zeolite trickling filter towers. After a 24 h culture under the optimal conditions, the viable count reached 4.76×109 cfu/mL, with the degradation rates of ammonia nitrogen (NH4+-N), nitrate nitrogen (NO3−-N), total nitrogen (TN), total phosphorus (TP), and chemical oxygen demand (COD) increased to 93.5%, 100%, 68.3%, 32.6%, and 85%, respectively. After optimizing the feeding strategy, the concentration of viable bacteria reached 5.80×109 cfu/mL. In the application effect verification experiment, the degradation rates of NH4+-N, TN, TP, and COD in the experimental group reached 96.69%, 75.18%, 73.82%, and 90.83%, respectively, showing a significant improvement compared to the results of the control group. The main components in the control group were Dokdonella, Brevundimonas, Alishewanella, Rhodobacter, Pseudoxanthomonas, and Thauera, whereas those in the experimental group were Dokdonella, Proteocatella, Rhodobacter, Dechlomonas, and Nitrospira. Proteocatella, Dechlomonas, and Nitrosra, which were unique to the experimental group, are common bacteria used for nitrogen and phosphorus removal. This explains the difference in the sewage treatment capacity between the two groups. This study provides an alternative sewage treatment microbial flora with a reasonable production cost and high degradation efficiency for NH4+-N, TN, TP, and COD.

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“…Therefore, we know that kaolinite can affect bacteria (McMahon et al, 2016). However, we do not know how kaolinite influences the composition of the bacterial communities within and surrounding a decaying carcass (Mähler et al, 2023), and the process by which kaolinite aids exceptional fossil preservation is yet to be fully deciphered (Naimark et al, 2016; Wilson & Butterfield, 2014).…”

Section: Introductionmentioning

confidence: 99%

The effects of clay minerals on bacterial community composition during arthropod decay

Corthésy,

Saleh,

Thomas

et al. 2024

Preprint

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Fossilization, or the transition of an organism from the biosphere to the geosphere, is a complex mechanism involving numerous biological and geological variables. Bacteria are one of the most significant biotic players to decompose organic matter in natural environments, early on during fossilization. However, bacterial processes are difficult to characterize as many different abiotic conditions can influence bacterial efficiency in degrading tissues. One potentially important variable is the composition and nature of the sediment on which a carcass is deposited after death. We experimentally examined this by decaying the marine shrimpPalaemon variansunderwater on three different clay sediments. Samples were then analyzed using 16S ribosomal RNA sequencing to identify the bacterial communities associated with each clay system. Results show that samples decaying on the surface of kaolinite have a lower bacterial diversity than those decaying on the surface of bentonite and montmorillonite, which could explain the limited decay of carcasses deposited on this clay. However, this is not the only role played by kaolinite, as a greater proportion of gram-negative over gram-positive bacteria is observed in this system. Gram-positive bacteria are generally thought to be more efficient at recycling complex polysaccharides such as those forming the body walls of arthropods. This is the first experimental evidence of sediments shaping an entire bacterial community. Such interaction between sediments and bacteria might have contributed to arthropods’ exquisite preservation and prevalence in kaolinite-rich Lagerstätten of the Cambrian Explosion.

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Time-dependent microbial shifts during crayfish decomposition in freshwater and sediment under different environmental conditions

Cited by 7 publications

References 96 publications

Oxygen-dependent biofilm dynamics in leaf decay: an in vitro analysis

Oxygen-dependent biofilm dynamics in leaf decay: an in vitro analysis

A microbial flora with superior pollutant removal efficiency and its fermentation process optimization

The effects of clay minerals on bacterial community composition during arthropod decay

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