Time-dependent hormesis of chemical mixtures: A case study on sulfa antibiotics and a quorum-sensing inhibitor of Vibrio fischeri

You, Ruirong; Sun, Haoyu; Yu, Yan; Zhao, Lin; Qin, Mengnan; Liu, Ying

doi:10.1016/j.etap.2015.10.013

Cited by 32 publications

(15 citation statements)

References 45 publications

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“…The exploitation of QS systems to search for virulence factor inhibitors has offered an alternative opportunity to control bacterial infections, given the fact that traditional antimicrobial treatments can easily become ineffective due to bacterial resistance. QS inhibitors have been shown to greatly impair bacterial infections, especially in the control of bio lm-related diseases [21,22]. We showed here that hypertonic glucose could inhibit growth, attenuate virulence factors, and regulate quorum sensing of multidrug-resistant P. aeruginosa.…”

Section: Discussionmentioning

confidence: 73%

Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

Chen

et al. 2020

Preprint

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Abstract Background: Pseudomonas aeruginosa is the most common Gram-negative pathogen responsible for chronic wound infections, such as diabetic foot infections, and further exacerbates the treatment options and cost of such conditions. Hypertonic glucose, a commonly used prolotherapy solution, can accelerate the proliferation of granulation tissue and improve microcirculation in wounds. However, the action of hypertonic glucose on bacterial pathogens that infect wounds is unclear. In this study, we investigated the inhibitory effects of hypertonic glucose on multidrug-resistant P. aeruginosa strains isolated from diabetic foot infections. Hypertonic glucose represents a novel approach to control chronic wound infections caused by P. aeruginosa. Results: Four multidrug-resistant P. aeruginosa clinical strains isolated from diabetic foot ulcers from a tertiary hospital in China and the reference P. aeruginosa PAO1 strain were studied. Hypertonic glucose significantly inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa clinical strains and PAO1. Furthermore, hypertonic glucose significantly reduced the production of pyocyanin and elastase virulence factors in P. aeruginosa. The expression of major quorum sensing genes (lasI, lasR, rhlI, and rhlR) in P. aeruginosa were all downregulated in response to hypertonic glucose treatment. In a Galleria mellonella larvae infection model, the administration of hypertonic glucose was shown to increase the survival rates of larvae infected by P. aeruginosa strains (3/5).Conclusions: Hypertonic glucose inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa, as well as reduced the production of virulence factors and quorum sensing gene expression. Further studies that investigate hypertonic glucose therapy should be considered in treating chronic wound infections.

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Section: Discussionmentioning

confidence: 73%

Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

Chen

et al. 2020

Preprint

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“…Numerous studies have demonstrated that hormesis is a common phenomenon in the laboratory and natural environment. 21 –24 As shown in Figure 4 B-I, with the concentration (−lgC (M)) of FA increases from −4.82 to −4.35, the inhibiting rate declines to negative. That is to say, FA enhances the bioluminescence.…”

Section: Resultsmentioning

confidence: 88%

“…25 Furaneol acetate can more easily bind to AinR protein than C8, leading to an increase in dissociative form of C8. 23 Next, C6 and C8 bind to LuxR protein, which induces a promotion on bioluminescence of A fischeri ( Figure 4B-III). This is why FA can stimulate the bioluminescence.…”

Section: Toxicity Tests For a Fischeri Over 24 Hoursmentioning

confidence: 98%

Time-Dependent Toxicities of Quorum Sensing Inhibitors toAliivibrio fischeriandBacillus subtilis

et al. 2019

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Quorum sensing inhibitors (QSIs) are being used widely as a promising alternative to antibiotics and drawing attention as potential pollutants. However, the assessment methods of the toxicities of QSIs, including model organism and affecting time, have not been established. To investigate how model organism and acting time impact the toxicities of QSIs, the effect of 4 QSIs to Aliivibrio fischeri and Bacillus subtilis were determined at different exposing time in the present study. The results showed that the toxic effects of QSIs to gram-negative bacteria ( A fischeri ) and gram-positive bacteria ( B subtilis ) were different and time dependent. As for A fischeri , QSI (furaneol acetate, FA) merely showed inhibition on the bioluminescence from hours 1 to 2. But from hours 3 to 6, low concentration FA exerted stimulation on the bioluminescence. Then, this stimulation disappeared from hours 7 to 14, and after hour 15 the stimulation appeared again. That is to say, QSIs showed intermittent hormesis effect on the bioluminescence of A fischeri . By contrast, only inhibition was observed in the toxicity test process of QSIs to B subtilis . As exposing time goes, the inhibition weakened gradually when FA was at low concentration regions. What is more, in the present, study toxic mechanisms were also discussed based on model organisms and exposing time. This study demonstrates appreciable impacts of model organism and exposing time on toxicities of QSIs and provides a theoretical basis for risk assessments after QSIs being widely used into the environment.

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“…The exploitation of QS systems to search for virulence factor inhibitors has offered an alternative opportunity to control bacterial infections, given the fact that traditional antimicrobial treatments can easily become ineffective due to bacterial resistance. QS inhibitors have been shown to greatly impair bacterial infections, especially in the control of biofilm-related diseases [ 21 , 22 ]. We showed here that hypertonic glucose could inhibit growth, attenuate virulence factors, and regulate quorum sensing of multidrug-resistant P. aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

Chen

et al. 2020

BMC Microbiol

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Background: Pseudomonas aeruginosa is the most common Gram-negative pathogen responsible for chronic wound infections, such as diabetic foot infections, and further exacerbates the treatment options and cost of such conditions. Hypertonic glucose, a commonly used prolotherapy solution, can accelerate the proliferation of granulation tissue and improve microcirculation in wounds. However, the action of hypertonic glucose on bacterial pathogens that infect wounds is unclear. In this study, we investigated the inhibitory effects of hypertonic glucose on multidrug-resistant P. aeruginosa strains isolated from diabetic foot infections. Hypertonic glucose represents a novel approach to control chronic wound infections caused by P. aeruginosa. Results: Four multidrug-resistant P. aeruginosa clinical strains isolated from diabetic foot ulcers from a tertiary hospital in China and the reference P. aeruginosa PAO1 strain were studied. Hypertonic glucose significantly inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa clinical strains and PAO1. Furthermore, hypertonic glucose significantly reduced the production of pyocyanin and elastase virulence factors in P. aeruginosa. The expression of major quorum sensing genes (lasI, lasR, rhlI, and rhlR) in P. aeruginosa were all downregulated in response to hypertonic glucose treatment. In a Galleria mellonella larvae infection model, the administration of hypertonic glucose was shown to increase the survival rates of larvae infected by P. aeruginosa strains (3/5). Conclusions: Hypertonic glucose inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa, as well as reduced the production of virulence factors and quorum sensing gene expression. Further studies that investigate hypertonic glucose therapy should be considered in treating chronic wound infections.

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Time-dependent hormesis of chemical mixtures: A case study on sulfa antibiotics and a quorum-sensing inhibitor of Vibrio fischeri

Cited by 32 publications

References 45 publications

Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

Time-Dependent Toxicities of Quorum Sensing Inhibitors toAliivibrio fischeriandBacillus subtilis

Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

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