Time-dependent effects of pyramidotomy in the operantly conditioned rats

Fanardjian, V.V.; Gevorkyan, O. V.; Mallina, Ravi; Melik-Moussian, A.B.; Meliksetyan, I. B.

doi:10.1016/s0166-4328(01)00159-0

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…However, in this experiment, we demonstrated that a transcortical approach with a small-diameter optical fiber is safe and effective for generating a circumscribed area of destruction in the medullary pyramid and persistent motor deficits. Models that use a ventral approach tend to show early recovery of motor deficits, but the deficits generated by our model persisted for a relatively long time after pyramidotomy[13–15]. The introduction of the optical fiber left a small track in the parenchyma of the brain; however, this track is quite far from both the dorsal and ventral groups of respiratory centers.…”

Section: Discussionmentioning

confidence: 97%

Transcortical photothrombotic pyramidotomy model with persistent motor deficits

et al. 2018

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Traditional pyramidotomy models have a high mortality rate from breathing difficulties and show early recovery from the induced motor deficits. This study establishes a novel pyramidotomy technique in Sprague Dawley rats that generates persistent motor deficits and has a reduced mortality rate. We used viral neural tracing to identify the course and relative distribution of forelimb and hindlimb motor fibers (n = 9). On basis of the neural tracing data, the medullary pyramid was targeted dorsally from the cerebellar cortex for photothrombotic infarct lesioning (n = 18). The photothrombotic technique selectively destroyed the corticospinal fibers in the medullary pyramid with relative preservation of neighboring grey-matter tissue. MicroPET imaging using 2-deoxy-2-[18F]-fluoro-D-glucose (FDG-microPET) showed a decrease in regional cerebral glucose metabolism (rCGM) in the bilateral pyramid and ipsilateral sensory cortex (p < 0.001, FDR q < 0.05). In addition, the trapezoid bodies and superior olivary nuclei showed a decrease in rCGM, compatible with damage caused during the introduction of the optical fiber. Connected structures such as the inferior colliculi and auditory cortices also showed decreases in rCGM in both hemispheres (p < 0.001, FDR q < 0.05). There was a significant and persistent decrease in motor and sensory function in the contralateral limb following pyramidotomy, as demonstrated by performance in the single pellet reaching task and the foot-fault test. There was no operative mortality or loss of respiratory function in this study. These results indicate that photothrombotic pyramidotomy with a dorsal transcortical approach is a safe and reliable technique for generating a pyramidotomy model with persistent motor deficits.

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Section: Discussionmentioning

confidence: 97%